APLF

Entrez Summary: N/A
UniProt Summary: Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic- apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. {ECO:0000269|PubMed:17353262, ECO:0000269|PubMed:17396150}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Global Fraction of Cell Lines Where Essential: 0/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Expression Distribution

Screen	Score
PF-06409577 20μM R07 exp389	2.07
Zebularine 20μM R07 exp423	2.09
Geldanamycin 0.015 to 0.025μM on day4 R07 exp316	2.2

positive regulation of DNA ligation
regulation of DNA ligation
single strand break repair
endodeoxyribonuclease activity
class I DNA-(apurinic or apyrimidinic site) endonuclease activity
DNA-(apurinic or apyrimidinic site) endonuclease activity
3-5 exonuclease activity
regulation of isotype switching
regulation of immunoglobulin mediated immune response
regulation of B cell mediated immunity
site of double-strand break
nucleotide binding
regulation of immunoglobulin production
regulation of DNA recombination
regulation of production of molecular mediator of immune response
regulation of adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains
regulation of lymphocyte mediated immunity
regulation of adaptive immune response
double-strand break repair
positive regulation of DNA metabolic process
regulation of leukocyte mediated immunity
regulation of B cell activation
nucleic acid phosphodiester bond hydrolysis
regulation of DNA metabolic process
regulation of immune effector process
DNA repair
regulation of lymphocyte activation
regulation of leukocyte activation
regulation of cell activation
DNA metabolic process
cellular response to DNA damage stimulus
regulation of immune response
regulation of immune system process
cellular response to stress
positive regulation of nucleobase-containing compound metabolic process