CHFR [The Human Chemical-Genetic Interaction Map Project]

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======= CHFR =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: CHFR
  * **<color #00a2e8>Official Name</color>**: checkpoint with forkhead and ring finger domains
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=55743|55743]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q96EP1|Q96EP1]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=CHFR&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20CHFR|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/605209|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014]. 
  * **<color #00a2e8>UniProt Summary</color>**:  E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. {ECO:0000269|PubMed:10935642, ECO:0000269|PubMed:11807090, ECO:0000269|PubMed:11912157, ECO:0000269|PubMed:14562038, ECO:0000269|PubMed:14694445, ECO:0000269|PubMed:18172500, ECO:0000269|PubMed:19182791}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|FHA|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|protein destabilization|
|nucleotide binding|
|positive regulation of proteasomal ubiquitin-dependent protein catabolic process|
|positive regulation of ubiquitin-dependent protein catabolic process|
|PML body|
|positive regulation of proteasomal protein catabolic process|
|positive regulation of protein ubiquitination|
|positive regulation of proteolysis involved in cellular protein catabolic process|
|regulation of proteasomal ubiquitin-dependent protein catabolic process|
|positive regulation of protein modification by small protein conjugation or removal|
|positive regulation of cellular protein catabolic process|
|regulation of ubiquitin-dependent protein catabolic process|
|mitotic cell cycle checkpoint|
|regulation of proteasomal protein catabolic process|
|cell cycle checkpoint|
|regulation of protein ubiquitination|
|positive regulation of protein catabolic process|
|regulation of proteolysis involved in cellular protein catabolic process|
|ubiquitin protein ligase activity|
|regulation of protein modification by small protein conjugation or removal|
|ubiquitin-protein transferase activity|
|regulation of cellular protein catabolic process|
|regulation of protein stability|
|protein polyubiquitination|
|negative regulation of mitotic cell cycle|
|positive regulation of proteolysis|
|positive regulation of cellular catabolic process|
|regulation of protein catabolic process|
|positive regulation of catabolic process|
|cell division|
|ubiquitin-dependent protein catabolic process|
|modification-dependent protein catabolic process|
|modification-dependent macromolecule catabolic process|
|negative regulation of cell cycle|
|proteolysis involved in cellular protein catabolic process|
|mitotic cell cycle process|
|cellular protein catabolic process|
|regulation of mitotic cell cycle|
|protein catabolic process|
|mitotic cell cycle|
|protein ubiquitination|
|regulation of proteolysis|
|protein modification by small protein conjugation|
|regulation of cellular catabolic process|
|cellular macromolecule catabolic process|
|protein modification by small protein conjugation or removal|
|regulation of catabolic process|
|cell cycle process|
|macromolecule catabolic process|
|organonitrogen compound catabolic process|
|regulation of cell cycle|
|positive regulation of protein modification process|
|proteolysis|
|cell cycle|
|positive regulation of cellular protein metabolic process|
|positive regulation of protein metabolic process|
|organic substance catabolic process|
|cellular catabolic process|
|regulation of protein modification process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp430|Rifampicin 30μM R08 exp430]]|-1.76|
|[[:results:exp29|Rapamycin 1μM R00 exp29]]|1.75|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 9126
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 4.31 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='CHFR Expression in NALM6 Cells: 4.31'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>