CHFR

Gene Information

Official Symbol: CHFR
Official Name: checkpoint with forkhead and ring finger domains
Aliases and Previous Symbols: N/A
Entrez ID: 55743
UniProt: Q96EP1
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014].
UniProt Summary: E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. {ECO:0000269|PubMed:10935642, ECO:0000269|PubMed:11807090, ECO:0000269|PubMed:11912157, ECO:0000269|PubMed:14562038, ECO:0000269|PubMed:14694445, ECO:0000269|PubMed:18172500, ECO:0000269|PubMed:19182791}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Global Fraction of Cell Lines Where Essential: 0/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 9126
Expression level (log2 read counts): 4.31

Expression Distribution

Screen	Score
Rifampicin 30μM R08 exp430	-1.76
Rapamycin 1μM R00 exp29	1.75

protein destabilization
nucleotide binding
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of ubiquitin-dependent protein catabolic process
PML body
positive regulation of proteasomal protein catabolic process
positive regulation of protein ubiquitination
positive regulation of proteolysis involved in cellular protein catabolic process
regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of protein modification by small protein conjugation or removal
positive regulation of cellular protein catabolic process
regulation of ubiquitin-dependent protein catabolic process
mitotic cell cycle checkpoint
regulation of proteasomal protein catabolic process
cell cycle checkpoint
regulation of protein ubiquitination
positive regulation of protein catabolic process
regulation of proteolysis involved in cellular protein catabolic process
ubiquitin protein ligase activity
regulation of protein modification by small protein conjugation or removal
ubiquitin-protein transferase activity
regulation of cellular protein catabolic process
regulation of protein stability
protein polyubiquitination
negative regulation of mitotic cell cycle
positive regulation of proteolysis
positive regulation of cellular catabolic process
regulation of protein catabolic process
positive regulation of catabolic process
cell division
ubiquitin-dependent protein catabolic process
modification-dependent protein catabolic process
modification-dependent macromolecule catabolic process
negative regulation of cell cycle
proteolysis involved in cellular protein catabolic process
mitotic cell cycle process
cellular protein catabolic process
regulation of mitotic cell cycle
protein catabolic process
mitotic cell cycle