NUDT15

Gene Information

Official Symbol: NUDT15
Official Name: nudix hydrolase 15
Aliases and Previous Symbols: N/A
Entrez ID: 55270
UniProt: Q9NV35
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: This gene encodes an enzyme that belongs to the Nudix hydrolase superfamily. Members of this superfamily catalyze the hydrolysis of nucleoside diphosphates, including substrates like 8-oxo-dGTP, which are a result of oxidative damage, and can induce base mispairing during DNA replication, causing transversions. The encoded enzyme is a negative regulator of thiopurine activation and toxicity. Mutations in this gene result in poor metabolism of thiopurines, and are associated with thiopurine-induced early leukopenia. Multiple pseudogenes of this gene have been identified. [provided by RefSeq, Apr 2016].
UniProt Summary: May catalyze the hydrolysis of nucleoside triphosphates including dGTP, dTTP, dCTP, their oxidized forms like 8-oxo-dGTP and the prodrug thiopurine derivatives 6-thio-dGTP and 6-thio-GTP (PubMed:26238318). Could also catalyze the hydrolysis of some nucleoside diphosphate derivatives (PubMed:22556419, PubMed:26238318). Hydrolyzes oxidized nucleosides triphosphates like 8-oxo-dGTP in vitro, but the specificity and efficiency towards these substrates are low. Therefore, the potential in vivo sanitizing role of this enzyme, that would consist in removing oxidatively damaged forms of nucleosides to prevent their incorporation into DNA, is unclear (PubMed:26238318, PubMed:22556419). Through the hydrolysis of thioguanosine triphosphates may participate in the catabolism of thiopurine drugs (PubMed:26238318, PubMed:25108385). May also have a role in DNA synthesis and cell cycle progression by stabilizing PCNA (PubMed:19419956). {ECO:0000269|PubMed:19419956, ECO:0000269|PubMed:22556419, ECO:0000269|PubMed:25108385, ECO:0000269|PubMed:26238318}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Global Fraction of Cell Lines Where Essential: 0/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 12309
Expression level (log2 read counts): 5.31

Expression Distribution

8-oxo-7,8-dihydrodeoxyguanosine triphosphate pyrophosphatase activity
8-oxo-7,8-dihydroguanosine triphosphate pyrophosphatase activity
dGTP catabolic process
dGTP metabolic process
nucleoside-diphosphatase activity
purine deoxyribonucleoside triphosphate catabolic process
DNA protection
purine deoxyribonucleotide catabolic process
purine nucleoside triphosphate catabolic process
nucleoside-triphosphate diphosphatase activity
NADH pyrophosphatase activity
purine deoxyribonucleoside triphosphate metabolic process
deoxyribonucleoside triphosphate catabolic process
purine deoxyribonucleotide metabolic process
nucleoside triphosphate catabolic process
deoxyribonucleoside triphosphate metabolic process
deoxyribonucleotide catabolic process
deoxyribose phosphate catabolic process
exogenous drug catabolic process
2-deoxyribonucleotide metabolic process
deoxyribose phosphate metabolic process
deoxyribonucleotide metabolic process
purine nucleotide catabolic process
nucleobase-containing small molecule catabolic process
purine-containing compound catabolic process
nucleotide catabolic process
purine nucleoside triphosphate metabolic process
nucleoside phosphate catabolic process
nucleoside triphosphate metabolic process
nucleobase-containing small molecule biosynthetic process
organophosphate catabolic process
drug catabolic process
regulation of proteasomal protein catabolic process
carbohydrate derivative catabolic process
response to reactive oxygen species
regulation of proteolysis involved in cellular protein catabolic process
regulation of cellular protein catabolic process
cellular response to extracellular stimulus
purine nucleotide metabolic process
cellular response to external stimulus