RNF146

Gene Information

Official Symbol: RNF146
Official Name: ring finger protein 146
Aliases and Previous Symbols: N/A
Entrez ID: 81847
UniProt: Q9NTX7
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination, leading to their degradation. Different ubiquitin linkage types have been observed: TNKS2 undergoes ubiquitination at 'Lys-48' and 'Lys-63', while AXIN1 is only ubiquitinated at 'Lys-48'. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation (By similarity). Protects against cell death induced by DNA damaging agents, such as N- methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair. {ECO:0000250, ECO:0000269|PubMed:21478859, ECO:0000269|PubMed:21602803, ECO:0000269|PubMed:21799911, ECO:0000269|PubMed:21825151, ECO:0000269|PubMed:22267412}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Ethanol 0.003 R08 exp437	-1.79
Epigallocatechin gallate 20μM R05 exp230	1.75
2-Methoxyestradiol 0.55μM R07 exp310	1.8
Fluvastatin 2.2μM R06 exp282	1.83
GSK461364A 0.005μM R03 exp125	1.85
Epothilone-D 0.004 to 0.006μM on day4 R05 exp232	1.88
AICAR 240μM R03 exp116	1.98
LY2090314 0.003μM R08 exp500 no dilution day6	2.48
LY2090314 0.003μM R08 exp499	2.61

Global Fraction of Cell Lines Where Essential: 1/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	1/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 8961
Expression level (log2 read counts): 3.96

Expression Distribution

poly-ADP-D-ribose binding
protein K48-linked ubiquitination
protein autoubiquitination
positive regulation of canonical Wnt signaling pathway
positive regulation of Wnt signaling pathway
ubiquitin protein ligase activity
ubiquitin-protein transferase activity
regulation of canonical Wnt signaling pathway
protein polyubiquitination
cell-cell signaling by wnt
Wnt signaling pathway
regulation of Wnt signaling pathway
cell surface receptor signaling pathway involved in cell-cell signaling
ubiquitin-dependent protein catabolic process
modification-dependent protein catabolic process
modification-dependent macromolecule catabolic process
proteolysis involved in cellular protein catabolic process
cellular protein catabolic process
protein catabolic process
protein ubiquitination
protein modification by small protein conjugation
zinc ion binding
cellular macromolecule catabolic process
protein modification by small protein conjugation or removal
macromolecule catabolic process
organonitrogen compound catabolic process
cell-cell signaling
proteolysis
positive regulation of signal transduction
organic substance catabolic process
cellular catabolic process
positive regulation of cell communication
positive regulation of signaling