SLC27A5

Entrez Summary: The protein encoded by this gene is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty-acids containing 24- and 26-carbons. It is expressed in liver and associated with endoplasmic reticulum but not with peroxisomes. Its primary role is in fatty acid elongation or complex lipid synthesis rather than in degradation. This gene has a mouse ortholog. [provided by RefSeq, Jul 2008].
UniProt Summary: Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7- alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. {ECO:0000269|PubMed:10749848, ECO:0000269|PubMed:11980911}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Global Fraction of Cell Lines Where Essential: 0/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Expression Distribution

Screen	Score
A-366 10μM R03 exp115	-2.21
LLY-283 2.6μM R06 exp290	1.71

long-chain fatty acid import across plasma membrane
cholate-CoA ligase activity
triglyceride mobilization
fatty acid transmembrane transporter activity
very long-chain fatty acid-CoA ligase activity
ketone body biosynthetic process
lipid import into cell
long-chain fatty acid import into cell
cellular ketone body metabolic process
long-chain fatty acid transporter activity
ketone body metabolic process
long-chain fatty acid-CoA ligase activity
fatty acid transmembrane transport
bile acid biosynthetic process
bile acid and bile salt transport
very long-chain fatty acid metabolic process
basal plasma membrane
bile acid metabolic process
long-chain fatty acid transport
fatty acid transport
triglyceride metabolic process
fatty acid derivative biosynthetic process
import across plasma membrane
acylglycerol metabolic process
neutral lipid metabolic process
integral component of endoplasmic reticulum membrane
long-chain fatty acid metabolic process
steroid biosynthetic process
organic acid transmembrane transport
carboxylic acid transmembrane transport
monocarboxylic acid transport
organic hydroxy compound transport
fatty acid derivative metabolic process
organic hydroxy compound biosynthetic process
monocarboxylic acid biosynthetic process
steroid metabolic process
protein-containing complex binding
anion transmembrane transport
carboxylic acid transport
organic acid transport