SPRTN [The Human Chemical-Genetic Interaction Map Project]

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======= SPRTN =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: SPRTN
  * **<color #00a2e8>Official Name</color>**: SprT-like N-terminal domain
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=83932|83932]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9H040|Q9H040]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=SPRTN&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20SPRTN|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/616086|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  The protein encoded by this gene may play a role in DNA repair during replication of damaged DNA. This protein recruits valosin containing protein (p97) to stalled DNA replication forks where it may prevent excessive translesional DNA synthesis and limit the number of DNA-damage induced mutations. It may also be involved in replication-related G2/M-checkpoint regulation. Deficiency of a similar protein in mouse causes chromosomal instability and progeroid phenotypes. Mutations in this gene have been associated with Ruijs-Aalfs syndrome (RJALS). Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Regulator of UV-induced DNA damage response: acts as a 'reader' of ubiquitinated PCNA that enhances RAD18-mediated PCNA ubiquitination and translesion DNA synthesis (TLS). Recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis. Acts as a regulator of TLS by recruiting VCP/p97 to sites of DNA damage, possibly leading to extraction of DNA polymerase eta (POLH) by VCP/p97 to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. {ECO:0000269|PubMed:22681887, ECO:0000269|PubMed:22894931, ECO:0000269|PubMed:22902628, ECO:0000269|PubMed:22987070, ECO:0000269|PubMed:23042605, ECO:0000269|PubMed:23042607}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|SprT-like|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|error-free translesion synthesis|
|K63-linked polyubiquitin modification-dependent protein binding|
|translesion synthesis|
|DNA synthesis involved in DNA repair|
|postreplication repair|
|ubiquitin binding|
|DNA biosynthetic process|
|positive regulation of protein ubiquitination|
|chromosome|
|positive regulation of protein modification by small protein conjugation or removal|
|response to UV|
|regulation of protein ubiquitination|
|regulation of protein modification by small protein conjugation or removal|
|response to light stimulus|
|nuclear speck|
|response to radiation|
|DNA repair|
|DNA metabolic process|
|cellular response to DNA damage stimulus|
|nucleobase-containing compound biosynthetic process|
|response to abiotic stimulus|
|heterocycle biosynthetic process|
|aromatic compound biosynthetic process|
|positive regulation of protein modification process|
|organic cyclic compound biosynthetic process|
|DNA binding|
|positive regulation of cellular protein metabolic process|
|cellular nitrogen compound biosynthetic process|
|cellular response to stress|
|positive regulation of protein metabolic process|
|cellular macromolecule biosynthetic process|
|macromolecule biosynthetic process|
|regulation of protein modification process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp505|ML-792 0.2μM R08 exp505]]|-1.89|
|[[:results:exp69|Deguelin 0.05μM R02 exp69]]|-1.75|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 112/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|5/28|
|blood|2/28|
|bone|5/25|
|breast|6/33|
|central nervous system|4/56|
|cervix|1/4|
|colorectal|6/17|
|esophagus|1/13|
|fibroblast|0/1|
|gastric|6/15|
|kidney|1/21|
|liver|2/20|
|lung|14/75|
|lymphocyte|0/14|
|ovary|4/26|
|pancreas|4/24|
|peripheral nervous system|3/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|1/7|
|thyroid|0/2|
|upper aerodigestive|4/22|
|urinary tract|3/29|
|uterus|2/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1824
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 4.77 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='SPRTN Expression in NALM6 Cells: 4.77'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>