SPRTN

Gene Information

Official Symbol: SPRTN
Official Name: SprT-like N-terminal domain
Aliases and Previous Symbols: N/A
Entrez ID: 83932
UniProt: Q9H040
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: The protein encoded by this gene may play a role in DNA repair during replication of damaged DNA. This protein recruits valosin containing protein (p97) to stalled DNA replication forks where it may prevent excessive translesional DNA synthesis and limit the number of DNA-damage induced mutations. It may also be involved in replication-related G2/M-checkpoint regulation. Deficiency of a similar protein in mouse causes chromosomal instability and progeroid phenotypes. Mutations in this gene have been associated with Ruijs-Aalfs syndrome (RJALS). Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015].
UniProt Summary: Regulator of UV-induced DNA damage response: acts as a 'reader' of ubiquitinated PCNA that enhances RAD18-mediated PCNA ubiquitination and translesion DNA synthesis (TLS). Recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis. Acts as a regulator of TLS by recruiting VCP/p97 to sites of DNA damage, possibly leading to extraction of DNA polymerase eta (POLH) by VCP/p97 to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. {ECO:0000269|PubMed:22681887, ECO:0000269|PubMed:22894931, ECO:0000269|PubMed:22902628, ECO:0000269|PubMed:22987070, ECO:0000269|PubMed:23042605, ECO:0000269|PubMed:23042607}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Global Fraction of Cell Lines Where Essential: 112/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	5/28
blood	2/28
bone	5/25
breast	6/33
central nervous system	4/56
cervix	1/4
colorectal	6/17
esophagus	1/13
fibroblast	0/1
gastric	6/15
kidney	1/21
liver	2/20
lung	14/75
lymphocyte	0/14
ovary	4/26
pancreas	4/24
peripheral nervous system	3/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	1/7
thyroid	0/2
upper aerodigestive	4/22
urinary tract	3/29
uterus	2/5

Essentiality in NALM6

Essentiality Rank: 1824
Expression level (log2 read counts): 4.77

Expression Distribution

Screen	Score
ML-792 0.2μM R08 exp505	-1.89
Deguelin 0.05μM R02 exp69	-1.75

error-free translesion synthesis
K63-linked polyubiquitin modification-dependent protein binding
translesion synthesis
DNA synthesis involved in DNA repair
postreplication repair
ubiquitin binding
DNA biosynthetic process
positive regulation of protein ubiquitination
chromosome
positive regulation of protein modification by small protein conjugation or removal
response to UV
regulation of protein ubiquitination
regulation of protein modification by small protein conjugation or removal
response to light stimulus
nuclear speck
response to radiation
DNA repair
DNA metabolic process
cellular response to DNA damage stimulus
nucleobase-containing compound biosynthetic process
response to abiotic stimulus
heterocycle biosynthetic process
aromatic compound biosynthetic process
positive regulation of protein modification process
organic cyclic compound biosynthetic process
DNA binding
positive regulation of cellular protein metabolic process
cellular nitrogen compound biosynthetic process
cellular response to stress
positive regulation of protein metabolic process
cellular macromolecule biosynthetic process
macromolecule biosynthetic process
regulation of protein modification process