UPF3A

Gene Information

Official Symbol: UPF3A
Official Name: UPF3A regulator of nonsense mediated mRNA decay
Aliases and Previous Symbols: N/A
Entrez ID: 65110
UniProt: Q9H1J1
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Several splice variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2017].
UniProt Summary: Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2- UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. Binds spliced mRNA upstream of exon-exon junctions. In vitro, weakly stimulates translation. {ECO:0000269|PubMed:11163187, ECO:0000269|PubMed:16601204}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Global Fraction of Cell Lines Where Essential: 10/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	1/22
urinary tract	2/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 3138
Expression level (log2 read counts): 6.58

Expression Distribution

exon-exon junction complex
telomeric DNA binding
structural constituent of nuclear pore
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
positive regulation of translation
positive regulation of cellular amide metabolic process
mRNA transport
nucleic acid transport
RNA transport
establishment of RNA localization
nuclear-transcribed mRNA catabolic process
mRNA catabolic process
RNA localization
nucleobase-containing compound transport
RNA catabolic process
nucleocytoplasmic transport
nuclear transport
regulation of translation
nucleobase-containing compound catabolic process
regulation of cellular amide metabolic process
heterocycle catabolic process
cellular nitrogen compound catabolic process
aromatic compound catabolic process
organic cyclic compound catabolic process
posttranscriptional regulation of gene expression
intracellular membrane-bounded organelle
mRNA metabolic process
nucleolus
cellular macromolecule catabolic process
macromolecule catabolic process
RNA binding
intracellular transport
positive regulation of cellular protein metabolic process
RNA metabolic process
positive regulation of protein metabolic process
negative regulation of gene expression
organic substance catabolic process
cellular catabolic process
establishment of localization in cell
nitrogen compound transport