Rifampicin 10μM R00 exp32 [The Human Chemical-Genetic Interaction Map Project]

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====== Rifampicin 10μM R00 exp32 ======
==== Mechanism of Action ====
Inhibits bacterial RNA polymerase, induces hepatic cytochrome P450 enzymes
  * **<color #00a2e8>Class / Subclass 1:</color>** Infectious Disease / Antibiotic




==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Rifampicin%%
    * **<color #00a2e8>Synonyms:</color>** Rifampin; Rifamycin AMP
    * **<color #00a2e8>CAS #:</color>** 13292-46-1
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/135398735|135398735]]
    * **<color #00a2e8>IUPAC:</color>** %%[(7S,9E,11S,12R,13S,14R,15R,16R,17S,18S,19E,21Z)-2,15,17,27,29-pentahydroxy-11-methoxy-3,7,12,14,16,18,22-heptamethyl-26-[(E)-(4-methylpiperazin-1-yl)iminomethyl]-6,23-dioxo-8,30-dioxa-24-azatetracyclo[23.3.1.14,7.05,28]triaconta-1(29),2,4,9,19,21,25,27-octaen-13-yl] acetate%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C43H58N4O12/c1-21-12-11-13-22(2)42(55)45-33-28(20-44-47-17-15-46(9)16-18-47)37(52)30-31(38(33)53)36(51)26(6)40-32(30)41(54)43(8,59-40)57-19-14-29(56-10)23(3)39(58-27(7)48)25(5)35(50)24(4)34(21)49/h11-14,19-21,23-25,29,34-35,39,49-53H,15-18H2,1-10H3,(H,45,55)/b12-11+,19-14+,22-13-,44-20+/t21-,23+,24+,25+,29-,34-,35+,39+,43-/m0/s1
    * **<color #00a2e8>INCHI Key:</color>** JQXXHWHPUNPDRT-WLSIYKJHSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 822.9
    * **<color #00a2e8>Canonical SMILES:</color>** CC1C=CC=C(C(=O)NC2=C(C(=C3C(=C2O)C(=C(C4=C3C(=O)C(O4)(OC=CC(C(C(C(C(C(C1O)C)O)C)OC(=O)C)C)OC)C)C)O)O)C=NN5CCN(CC5)C)C
    * **<color #00a2e8>Isomeric SMILES:</color>** C[C@H]1/C=C/C=C(\\C(=O)NC2=C(C(=C3C(=C2O)C(=C(C4=C3C(=O)[C@](O4)(O/C=C/[C@@H]([C@H]([C@H]([C@@H]([C@@H]([C@@H]([C@H]1O)C)O)C)OC(=O)C)C)OC)C)C)O)O)/C=N/N5CCN(CC5)C)/C
    * **<color #00a2e8>Molecular Formula:</color>** C43H58N4O12
{{:chemogenomics:structures:chem-0017.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Med Chem Express
    * **<color #00a2e8>Catalog #:</color>** HY-B0272
    * **<color #00a2e8>Lot #:</color>** 33587
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C43H58N4O12 823.4124; found 823.4128
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Rifampicin
    * **<color #00a2e8>Min:</color>** -17.8934; **<color #00a2e8>Max:</color>** 5.5857
{{:chemogenomics:dose_response:dr_274.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |N/A    |
| IC30  |N/A    |
| IC40  |N/A    |
| IC50  |N/A    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 00
    * **<color #00a2e8>Dose</color>**: 10µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 6.1
    * **<color #00a2e8>Numbers of reads</color>**: 9970595


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|3/2|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Rifampicin_10uM_Round-0_exp32.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Rifampicin 10μM R00 exp32'>
{{:chemogenomics:cranks_plots:exp32.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:S:SEPHS1|SEPHS1]]|-3.13|<0.01|
|[[:human_genes:R:RSRC2|RSRC2]]|-2.83|0.30|
|[[:human_genes:N:NUDT5|NUDT5]]|-2.70|0.05|
|[[:human_genes:M:MSI2|MSI2]]|-2.65|0.05|
|[[:human_genes:I:ILF3|ILF3]]|-2.52|0.30|
|[[:human_genes:C:C3orf43|C3orf43]]|-2.51|0.09|
|[[:human_genes:N:NPRL3|NPRL3]]|-2.47|0.28|
|[[:human_genes:P:PDAP1|PDAP1]]|-2.46|0.09|
|[[:human_genes:F:FOXE3|FOXE3]]|-2.35|0.14|
|[[:human_genes:N:NME6|NME6]]|-2.33|0.14|
|[[:human_genes:S:SZT2|SZT2]]|-2.26|0.19|
|[[:human_genes:M:MC5R|MC5R]]|-2.19|0.30|
|[[:human_genes:M:MAPKAP1|MAPKAP1]]|-2.19|0.30|
|[[:human_genes:F:FAF2|FAF2]]|-2.13|0.34|
|[[:human_genes:D:DCAF15|DCAF15]]|-2.13|0.34|
|[[:human_genes:U:UNK|UNK]]|-2.10|0.30|
|[[:human_genes:D:DNTTIP1|DNTTIP1]]|-2.10|0.30|
|[[:human_genes:O:OIP5|OIP5]]|-2.09|0.30|
|[[:human_genes:M:MUC6|MUC6]]|-2.09|0.45|
|[[:human_genes:P:PADI2|PADI2]]|-2.07|0.38|
|[[:human_genes:M:MARS|MARS]]|-2.04|0.62|
|[[:human_genes:A:ASB3|ASB3]]|-2.01|0.34|
|[[:human_genes:C:CCDC24|CCDC24]]|-2.00|0.57|
|[[:human_genes:R:RREB1|RREB1]]|-1.99|0.36|
|[[:human_genes:K:KIF23|KIF23]]|-1.98|0.36|
|[[:human_genes:M:MROH8|MROH8]]|-1.97|0.55|
|[[:human_genes:F:FRRS1|FRRS1]]|-1.97|0.37|
|[[:human_genes:C:C12orf66|C12orf66]]|-1.94|0.39|
|[[:human_genes:S:SH3GL1|SH3GL1]]|-1.94|0.56|
|[[:human_genes:N:NPIPA2|NPIPA2]]|-1.94|0.62|
|[[:human_genes:C:CDK2|CDK2]]|2.10|0.27|
|[[:human_genes:M:MFSD6|MFSD6]]|2.13|0.22|
|[[:human_genes:O:ORC1|ORC1]]|2.14|0.33|
|[[:human_genes:C:CCNT1|CCNT1]]|2.14|0.22|
|[[:human_genes:A:ABO|ABO]]|2.15|0.38|
|[[:human_genes:T:TM4SF20|TM4SF20]]|2.16|0.22|
|[[:human_genes:S:SMIM15|SMIM15]]|2.17|0.22|
|[[:human_genes:P:PDC|PDC]]|2.19|0.25|
|[[:human_genes:M:MYCBP2|MYCBP2]]|2.20|0.25|
|[[:human_genes:T:TRPA1|TRPA1]]|2.22|0.22|
|[[:human_genes:P:PNPLA8|PNPLA8]]|2.23|0.28|
|[[:human_genes:Z:ZNF106|ZNF106]]|2.24|0.23|
|[[:human_genes:C:CCL16|CCL16]]|2.26|0.27|
|[[:human_genes:T:TMEM165|TMEM165]]|2.26|0.22|
|[[:human_genes:W:WDR43|WDR43]]|2.26|0.39|
|[[:human_genes:P:PSORS1C1|PSORS1C1]]|2.29|0.22|
|[[:human_genes:R:RXFP2|RXFP2]]|2.31|0.22|
|[[:human_genes:C:CTF1|CTF1]]|2.34|0.22|
|[[:human_genes:F:FKBP15|FKBP15]]|2.38|0.17|
|[[:human_genes:N:NCBP1|NCBP1]]|2.44|0.27|
|[[:human_genes:A:ANKRD40|ANKRD40]]|2.45|0.22|
|[[:human_genes:P:PSMD7|PSMD7]]|2.48|0.33|
|[[:human_genes:K:KREMEN1|KREMEN1]]|2.65|0.06|
|[[:human_genes:N:NUP153|NUP153]]|2.69|0.17|
|[[:human_genes:R:RPL14|RPL14]]|2.70|0.26|
|[[:human_genes:C:C10orf53|C10orf53]]|2.85|0.02|
|[[:human_genes:P:PSMD1|PSMD1]]|2.93|0.27|
|[[:human_genes:S:SNRPF|SNRPF]]|2.98|0.26|
|[[:human_genes:A:ATP2C1|ATP2C1]]|3.16|<0.01|
|[[:human_genes:R:RPS28|RPS28]]|3.33|0.22|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp37|Wortmannin 0.5μM R00 exp37]]|0.074|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp16|DABN 2μM R00 exp16]]|0.07|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp15|Cycloheximide 0.2μM R00 exp15]]|0.061|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp310|2-Methoxyestradiol 0.55μM R07 exp310]]|0.056|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp5|Alpha-Amanitin 0.5μM R00 exp5]]|0.053|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp179|Combretastatin A4 0.002 to 0.003μM day4 R04 exp179]]|0.051|<button type="primary" size="small" modal="6">View</button>|
</modal>


<modal id="1" size="lg" title="Rifampicin 10μM R00 exp32  vs  Wortmannin 0.5μM R00 exp37">
{{:chemogenomics:comparison_plots:exp32_vs_exp37.png?nolink |}}
</modal>

<modal id="2" size="lg" title="DABN 2μM R00 exp16  vs  Rifampicin 10μM R00 exp32">
{{:chemogenomics:comparison_plots:exp16_vs_exp32.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Cycloheximide 0.2μM R00 exp15  vs  Rifampicin 10μM R00 exp32">
{{:chemogenomics:comparison_plots:exp15_vs_exp32.png?nolink |}}
</modal>

<modal id="4" size="lg" title="2-Methoxyestradiol 0.55μM R07 exp310  vs  Rifampicin 10μM R00 exp32">
{{:chemogenomics:comparison_plots:exp310_vs_exp32.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Alpha-Amanitin 0.5μM R00 exp5  vs  Rifampicin 10μM R00 exp32">
{{:chemogenomics:comparison_plots:exp32_vs_exp5.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Combretastatin A4 0.002 to 0.003μM day4 R04 exp179  vs  Rifampicin 10μM R00 exp32">
{{:chemogenomics:comparison_plots:exp179_vs_exp32.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>