Latrunculin-B 10μM R07 exp374 [The Human Chemical-Genetic Interaction Map Project]

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====== Latrunculin-B 10μM R07 exp374 ======
==== Mechanism of Action ====
Binds actin monomers, inhibits actin polymerization
  * **<color #00a2e8>Class / Subclass 1:</color>** Cell Cycle / Cytokinesis Inhibitor
  * **<color #00a2e8>Class / Subclass 2:</color>** Organelle Function / Cytoskeletal Inhibitor
  * **<color #00a2e8>Class / Subclass 3:</color>** Environmental Stresses / Toxin


==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Latrunculin B%%
    * **<color #00a2e8>Synonyms:</color>** N/A
    * **<color #00a2e8>CAS #:</color>** 76343-94-7
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/6436219|6436219]]
    * **<color #00a2e8>IUPAC:</color>** %%(4R)-4-[(1R,4Z,8Z,10S,13R,15R)-15-hydroxy-5,10-dimethyl-3-oxo-2,14-dioxabicyclo[11.3.1]heptadeca-4,8-dien-15-yl]-1,3-thiazolidin-2-one%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C20H29NO5S/c1-13-5-3-4-6-14(2)9-18(22)25-16-10-15(8-7-13)26-20(24,11-16)17-12-27-19(23)21-17/h3,5,9,13,15-17,24H,4,6-8,10-12H2,1-2H3,(H,21,23)/b5-3-,14-9-/t13-,15-,16-,17+,20-/m1/s1
    * **<color #00a2e8>INCHI Key:</color>** NSHPHXHGRHSMIK-JRIKCGFMSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 395.5
    * **<color #00a2e8>Canonical SMILES:</color>** CC1CCC2CC(CC(O2)(C3CSC(=O)N3)O)OC(=O)C=C(CCC=C1)C
    * **<color #00a2e8>Isomeric SMILES:</color>** C[C@H]/1CC[C@@H]2C[C@H](C[C@@](O2)([C@@H]3CSC(=O)N3)O)OC(=O)/C=C(\\CC/C=C1)/C
    * **<color #00a2e8>Molecular Formula:</color>** C20H29NO5S
{{:chemogenomics:structures:chem-0221.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Abcam
    * **<color #00a2e8>Catalog #:</color>** ab144291
    * **<color #00a2e8>Lot #:</color>** GR3184664-5
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+Na)+ Calcd for C20H29NO5S 418.16586; found 418.16584
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Latrunculin_B
    * **<color #00a2e8>Min:</color>** -3.9960; **<color #00a2e8>Max:</color>** 98.0418
{{:chemogenomics:dose_response:dr_389.png?nolink&500 |}}
\\ 
\\ 
\\ 
\\ 
|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |2.0673    |
| IC30  |4.2107    |
| IC40  |7.7555    |
| IC50  |13.8130    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 07
    * **<color #00a2e8>Dose</color>**: 10µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: -0.2
    * **<color #00a2e8>Numbers of reads</color>**: 21695338


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|1/95|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Latrunculin_B_10uM_Round-7_exp374.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Latrunculin-B 10μM R07 exp374'>
{{:chemogenomics:cranks_plots:exp374.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:Z:ZFAND5|ZFAND5]]|-2.97|0.02|
|[[:human_genes:S:SRSF6|SRSF6]]|-2.73|0.09|
|[[:human_genes:T:TAF5L|TAF5L]]|-2.60|0.11|
|[[:human_genes:C:CHMP7|CHMP7]]|-2.53|0.09|
|[[:human_genes:M:MCTS1|MCTS1]]|-2.51|0.09|
|[[:human_genes:G:GPBP1|GPBP1]]|-2.48|0.28|
|[[:human_genes:H:H2AFV|H2AFV]]|-2.32|0.19|
|[[:human_genes:O:OTUB1|OTUB1]]|-2.29|0.28|
|[[:human_genes:C:C1orf131|C1orf131]]|-2.28|0.31|
|[[:human_genes:L:LEMD3|LEMD3]]|-2.27|0.28|
|[[:human_genes:F:FAM78B|FAM78B]]|-2.25|0.25|
|[[:human_genes:A:AZIN1|AZIN1]]|-2.18|0.28|
|[[:human_genes:P:PHF1|PHF1]]|-2.18|0.31|
|[[:human_genes:E:EP300|EP300]]|-2.18|0.38|
|[[:human_genes:G:GZF1|GZF1]]|-2.17|0.35|
|[[:human_genes:H:HNRNPA2B1|HNRNPA2B1]]|-2.16|0.35|
|[[:human_genes:I:IRF4|IRF4]]|-2.13|0.28|
|[[:human_genes:D:DOT1L|DOT1L]]|-2.11|0.33|
|[[:human_genes:C:CLASP1|CLASP1]]|-2.11|0.29|
|[[:human_genes:P:PDE7B|PDE7B]]|-2.10|0.33|
|[[:human_genes:G:GPX4|GPX4]]|-2.08|0.31|
|[[:human_genes:H:H2AFJ|H2AFJ]]|-2.08|0.35|
|[[:human_genes:B:BRD8|BRD8]]|-2.04|0.35|
|[[:human_genes:H:HDAC1|HDAC1]]|-2.04|0.31|
|[[:human_genes:S:SACM1L|SACM1L]]|-2.02|0.31|
|[[:human_genes:A:ATXN7|ATXN7]]|-2.01|0.31|
|[[:human_genes:T:TFF1|TFF1]]|-2.01|0.31|
|[[:human_genes:K:KCMF1|KCMF1]]|-2.01|0.31|
|[[:human_genes:X:XPR1|XPR1]]|-2.01|0.31|
|[[:human_genes:C:CFAP45|CFAP45]]|-2.00|0.73|
|[[:human_genes:S:SCLT1|SCLT1]]|3.72|0.00|
|[[:human_genes:W:WBP11|WBP11]]|3.72|0.00|
|[[:human_genes:A:ASUN|ASUN]]|3.74|0.00|
|[[:human_genes:H:HSPA4|HSPA4]]|3.79|0.00|
|[[:human_genes:Z:ZFC3H1|ZFC3H1]]|3.95|0.00|
|[[:human_genes:T:TUBE1|TUBE1]]|4.03|0.00|
|[[:human_genes:B:BAK1|BAK1]]|4.15|0.00|
|[[:human_genes:R:RBM26|RBM26]]|4.35|0.00|
|[[:human_genes:F:FOPNL|FOPNL]]|4.41|0.00|
|[[:human_genes:C:C16orf59|C16orf59]]|4.50|0.00|
|[[:human_genes:E:EIF4A1|EIF4A1]]|4.54|0.00|
|[[:human_genes:N:NIN|NIN]]|4.64|0.00|
|[[:human_genes:B:BUB3|BUB3]]|4.81|0.00|
|[[:human_genes:T:TUBD1|TUBD1]]|5.16|0.00|
|[[:human_genes:C:CEP120|CEP120]]|5.17|0.00|
|[[:human_genes:C:C14orf80|C14orf80]]|5.28|0.00|
|[[:human_genes:A:ANKRD26|ANKRD26]]|5.37|0.00|
|[[:human_genes:C:CEP78|CEP78]]|5.50|0.00|
|[[:human_genes:C:CRADD|CRADD]]|5.52|0.00|
|[[:human_genes:C:CEP44|CEP44]]|5.54|0.00|
|[[:human_genes:O:ODF2|ODF2]]|5.57|0.00|
|[[:human_genes:K:KIAA0753|KIAA0753]]|5.68|0.00|
|[[:human_genes:C:CEP76|CEP76]]|5.70|0.00|
|[[:human_genes:O:OFD1|OFD1]]|5.82|0.00|
|[[:human_genes:C:C2CD3|C2CD3]]|6.04|0.00|
|[[:human_genes:C:CEP128|CEP128]]|6.41|0.00|
|[[:human_genes:P:PIBF1|PIBF1]]|6.43|0.00|
|[[:human_genes:P:PIDD|PIDD]]|6.50|0.00|
|[[:human_genes:C:CASP2|CASP2]]|6.66|0.00|
|[[:human_genes:C:CCDC41|CCDC41]]|7.65|0.00|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp414|Tozasertib 0.1μM R07 exp414]]|0.251|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp80|RO-3307 4.7μM R02 exp80]]|0.129|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp524|Staurosporine 0.02μM R08 exp524]]|0.078|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp512|Olaparib 4μM R08 exp512]]|0.072|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp134|MS023 2μM R03 exp134]]|0.067|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp135|MS023 7μM R03 exp135]]|0.065|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp505|ML-792 0.2μM R08 exp505]]|0.056|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp240|Pyridostatin 4μM R05 exp240]]|0.054|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp503|Mitomycin-C 0.06μM R08 exp503]]|0.054|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp535|Trimetrexate 0.03μM R08 exp535]]|0.053|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp349|Cytochalasin-B 5μM R07 exp349]]|0.273|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|0.076|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp343|Centrinone 0.5μM R07 exp343]]|0.071|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp234|Ethanol 0.01 R05 exp234]]|0.069|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|0.069|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp485|GSK626616 14μM R08 exp485]]|0.068|<button type="primary" size="small" modal="16">View</button>|
|[[:results:exp474|CR131-b 0.005μM R08 exp474]]|0.061|<button type="primary" size="small" modal="17">View</button>|
|[[:results:exp211|AICAR 240μM R05 exp211]]|0.061|<button type="primary" size="small" modal="18">View</button>|
|[[:results:exp478|Doxorubicin 0.02μM R08 exp478]]|0.059|<button type="primary" size="small" modal="19">View</button>|
|[[:results:exp440|Aphidicolin 0.4μM R08 exp440]]|0.058|<button type="primary" size="small" modal="20">View</button>|
|[[:results:exp468|CB-5083 0.4μM R08 exp468]]|0.055|<button type="primary" size="small" modal="21">View</button>|
|[[:results:exp199|Etoposide 0.3μM R05 exp199]]|0.054|<button type="primary" size="small" modal="22">View</button>|
|[[:results:exp116|AICAR 240μM R03 exp116]]|0.053|<button type="primary" size="small" modal="23">View</button>|
|[[:results:exp456|Benzoate 20000μM R08 exp456]]|0.052|<button type="primary" size="small" modal="24">View</button>|
</modal>


<modal id="1" size="lg" title="Latrunculin-B 10μM R07 exp374  vs  Tozasertib 0.1μM R07 exp414">
{{:chemogenomics:comparison_plots:exp374_vs_exp414.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Latrunculin-B 10μM R07 exp374  vs  RO-3307 4.7μM R02 exp80">
{{:chemogenomics:comparison_plots:exp374_vs_exp80.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Latrunculin-B 10μM R07 exp374  vs  Staurosporine 0.02μM R08 exp524">
{{:chemogenomics:comparison_plots:exp374_vs_exp524.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Latrunculin-B 10μM R07 exp374  vs  Olaparib 4μM R08 exp512">
{{:chemogenomics:comparison_plots:exp374_vs_exp512.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Latrunculin-B 10μM R07 exp374  vs  MS023 2μM R03 exp134">
{{:chemogenomics:comparison_plots:exp134_vs_exp374.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Latrunculin-B 10μM R07 exp374  vs  MS023 7μM R03 exp135">
{{:chemogenomics:comparison_plots:exp135_vs_exp374.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Latrunculin-B 10μM R07 exp374  vs  ML-792 0.2μM R08 exp505">
{{:chemogenomics:comparison_plots:exp374_vs_exp505.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Latrunculin-B 10μM R07 exp374  vs  Pyridostatin 4μM R05 exp240">
{{:chemogenomics:comparison_plots:exp240_vs_exp374.png?nolink |}}
</modal>

<modal id="9" size="lg" title="Latrunculin-B 10μM R07 exp374  vs  Mitomycin-C 0.06μM R08 exp503">
{{:chemogenomics:comparison_plots:exp374_vs_exp503.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Latrunculin-B 10μM R07 exp374  vs  Trimetrexate 0.03μM R08 exp535">
{{:chemogenomics:comparison_plots:exp374_vs_exp535.png?nolink |}}
</modal>

<modal id="11" size="lg" title="Cytochalasin-B 5μM R07 exp349  vs  Latrunculin-B 10μM R07 exp374">
{{:chemogenomics:comparison_plots:exp349_vs_exp374.png?nolink |}}
</modal>

<modal id="12" size="lg" title="Hippuristanol 0.12μM R08 exp489 no dilution day6  vs  Latrunculin-B 10μM R07 exp374">
{{:chemogenomics:comparison_plots:exp374_vs_exp489.png?nolink |}}
</modal>

<modal id="13" size="lg" title="Centrinone 0.5μM R07 exp343  vs  Latrunculin-B 10μM R07 exp374">
{{:chemogenomics:comparison_plots:exp343_vs_exp374.png?nolink |}}
</modal>

<modal id="14" size="lg" title="Ethanol 0.01 R05 exp234  vs  Latrunculin-B 10μM R07 exp374">
{{:chemogenomics:comparison_plots:exp234_vs_exp374.png?nolink |}}
</modal>

<modal id="15" size="lg" title="Hippuristanol 0.12μM R08 exp488  vs  Latrunculin-B 10μM R07 exp374">
{{:chemogenomics:comparison_plots:exp374_vs_exp488.png?nolink |}}
</modal>

<modal id="16" size="lg" title="GSK626616 14μM R08 exp485  vs  Latrunculin-B 10μM R07 exp374">
{{:chemogenomics:comparison_plots:exp374_vs_exp485.png?nolink |}}
</modal>

<modal id="17" size="lg" title="CR131-b 0.005μM R08 exp474  vs  Latrunculin-B 10μM R07 exp374">
{{:chemogenomics:comparison_plots:exp374_vs_exp474.png?nolink |}}
</modal>

<modal id="18" size="lg" title="AICAR 240μM R05 exp211  vs  Latrunculin-B 10μM R07 exp374">
{{:chemogenomics:comparison_plots:exp211_vs_exp374.png?nolink |}}
</modal>

<modal id="19" size="lg" title="Doxorubicin 0.02μM R08 exp478  vs  Latrunculin-B 10μM R07 exp374">
{{:chemogenomics:comparison_plots:exp374_vs_exp478.png?nolink |}}
</modal>

<modal id="20" size="lg" title="Aphidicolin 0.4μM R08 exp440  vs  Latrunculin-B 10μM R07 exp374">
{{:chemogenomics:comparison_plots:exp374_vs_exp440.png?nolink |}}
</modal>

<modal id="21" size="lg" title="CB-5083 0.4μM R08 exp468  vs  Latrunculin-B 10μM R07 exp374">
{{:chemogenomics:comparison_plots:exp374_vs_exp468.png?nolink |}}
</modal>

<modal id="22" size="lg" title="Etoposide 0.3μM R05 exp199  vs  Latrunculin-B 10μM R07 exp374">
{{:chemogenomics:comparison_plots:exp199_vs_exp374.png?nolink |}}
</modal>

<modal id="23" size="lg" title="AICAR 240μM R03 exp116  vs  Latrunculin-B 10μM R07 exp374">
{{:chemogenomics:comparison_plots:exp116_vs_exp374.png?nolink |}}
</modal>

<modal id="24" size="lg" title="Benzoate 20000μM R08 exp456  vs  Latrunculin-B 10μM R07 exp374">
{{:chemogenomics:comparison_plots:exp374_vs_exp456.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|pre-mRNA binding|54.18|SRSF6,EP300,HNRNPA2B1|
|internal protein amino acid acetylation|49.79|TAF5L,EP300,BRD8|
|peptidyl-lysine acetylation|48.48|TAF5L,EP300,BRD8|
|histone modification|36.99|TAF5L,EP300,DOT1L,BRD8,HDAC1|
|protein-DNA complex organization|6.27|H2AZ2,PHF1,EP300,IRF4,DOT1L,GPX4,BRD8,HDAC1|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|ciliary transition fiber|236.84|CEP83,NIN,SCLT1|
|centriolar subdistal appendage|184.21|CEP128,ODF2,NIN|
|protein localization to centrosome|100.48|CEP83,PIBF1,C2CD3,KIAA0753|
|centriole replication|92.11|C2CD3,OFD1,KIAA0753,CEP44|
|centriole assembly|71.31|C2CD3,OFD1,KIAA0753,CEP44|
|centrosome duplication|63.16|C2CD3,OFD1,KIAA0753,CEP44|
|protein localization to microtubule cytoskeleton|59.74|CEP83,PIBF1,C2CD3,KIAA0753|
|centriole|52.63|CEP83,CEP128,C2CD3,OFD1,CEP76,KIAA0753,ODF2,CEP44,CEP78,CEP120,TUBD1,NIN,CEP20,SCLT1|
|centrosome cycle|49.12|C2CD3,OFD1,KIAA0753,ODF2,CEP44,CEP120,NIN,TUBE1|
|microtubule organizing center organization|41.32|C2CD3,OFD1,KIAA0753,ODF2,CEP44,CEP120,NIN,TUBE1|
|cilium organization|15.40|CEP83,PIBF1,C2CD3,OFD1,KIAA0753,ODF2,CEP78,CEP20,SCLT1|
|centrosome|14.40|CEP83,PIBF1,CEP128,C2CD3,OFD1,CEP76,KIAA0753,ODF2,CEP44,CEP78,ANKRD26,CEP120,NIN,CEP20,TUBE1,SCLT1|
|cilium assembly|13.11|CEP83,PIBF1,C2CD3,OFD1,KIAA0753,CEP20,SCLT1|
|microtubule cytoskeleton organization|12.33|PIBF1,C2CD3,OFD1,KIAA0753,ODF2,CEP44,CEP120,TUBD1,NIN,CEP20,TUBE1,INTS13|
|microtubule organizing center|11.67|CEP83,PIBF1,CEP128,C2CD3,OFD1,CEP76,KIAA0753,ODF2,CEP44,CEP78,ANKRD26,CEP120,TUBD1,NIN,CEP20,TUBE1,SCLT1|
|cell cycle process|8.87|CASP2,PIDD1,PIBF1,C2CD3,OFD1,KIAA0753,ODF2,CEP44,CRADD,CEP120,BUB3,NIN,TUBE1,INTS13|
|microtubule-based process|8.51|PIBF1,C2CD3,OFD1,KIAA0753,ODF2,CEP44,CEP120,TUBD1,NIN,CEP20,TUBE1,INTS13|
|mitotic cell cycle|8.29|CASP2,PIDD1,PIBF1,OFD1,CRADD,TUBD1,BUB3,TUBE1,INTS13|
|cilium|8.14|CEP83,CEP128,C2CD3,OFD1,ODF2,CEP78,TUBD1,NIN,CEP20,SCLT1|
</modal>