Latrunculin-B 10μM R07 exp374

Binds actin monomers, inhibits actin polymerization

Class / Subclass 1: Cell Cycle / Cytokinesis Inhibitor
Class / Subclass 2: Organelle Function / Cytoskeletal Inhibitor
Class / Subclass 3: Environmental Stresses / Toxin

Compound References

PubChem Name: Latrunculin B
Synonyms: N/A
CAS #: 76343-94-7
PubChem CID: 6436219
IUPAC: (4R)-4-[(1R,4Z,8Z,10S,13R,15R)-15-hydroxy-5,10-dimethyl-3-oxo-2,14-dioxabicyclo[11.3.1]heptadeca-4,8-dien-15-yl]-1,3-thiazolidin-2-one
INCHI Name: InChI=1S/C20H29NO5S/c1-13-5-3-4-6-14(2)9-18(22)25-16-10-15(8-7-13)26-20(24,11-16)17-12-27-19(23)21-17/h3,5,9,13,15-17,24H,4,6-8,10-12H2,1-2H3,(H,21,23)/b5-3-,14-9-/t13-,15-,16-,17+,20-/m1/s1
INCHI Key: NSHPHXHGRHSMIK-JRIKCGFMSA-N
Molecular Weight: 395.5
Canonical SMILES: CC1CCC2CC(CC(O2)(C3CSC(=O)N3)O)OC(=O)C=C(CCC=C1)C
Isomeric SMILES: C[C@H]/1CC[C@@H]2C[C@H](C[C@@](O2)([C@@H]3CSC(=O)N3)O)OC(=O)/C=C(\\CC/C=C1)/C
Molecular Formula: C20H29NO5S

Compound Supplier

Supplier Name: Abcam
Catalog #: ab144291
Lot #: GR3184664-5

Compound Characterization

HRMS (ESI-TOF) m/z: (M+Na)+ Calcd for C20H29NO5S 418.16586; found 418.16584

Dose Response Curve

Platform ID: Latrunculin_B
Min: -3.9960; Max: 98.0418

IC	Concentration (µM)
IC10	N/A
IC20	2.0673
IC30	4.2107
IC40	7.7555
IC50	13.8130
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 07
Dose: 10µM
Days of incubation: 8
Doublings: -0.2
Numbers of reads: 21695338

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
1/95	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
ZFAND5	-2.97	0.02
SRSF6	-2.73	0.09
TAF5L	-2.60	0.11
CHMP7	-2.53	0.09
MCTS1	-2.51	0.09
GPBP1	-2.48	0.28
H2AFV	-2.32	0.19
OTUB1	-2.29	0.28
C1orf131	-2.28	0.31
LEMD3	-2.27	0.28
FAM78B	-2.25	0.25
AZIN1	-2.18	0.28
PHF1	-2.18	0.31
EP300	-2.18	0.38
GZF1	-2.17	0.35
HNRNPA2B1	-2.16	0.35
IRF4	-2.13	0.28
DOT1L	-2.11	0.33
CLASP1	-2.11	0.29
PDE7B	-2.10	0.33
GPX4	-2.08	0.31
H2AFJ	-2.08	0.35
BRD8	-2.04	0.35
HDAC1	-2.04	0.31
SACM1L	-2.02	0.31
ATXN7	-2.01	0.31
TFF1	-2.01	0.31
KCMF1	-2.01	0.31
XPR1	-2.01	0.31
CFAP45	-2.00	0.73
SCLT1	3.72	0.00
WBP11	3.72	0.00
ASUN	3.74	0.00
HSPA4	3.79	0.00
ZFC3H1	3.95	0.00
TUBE1	4.03	0.00
BAK1	4.15	0.00
RBM26	4.35	0.00
FOPNL	4.41	0.00
C16orf59	4.50	0.00
EIF4A1	4.54	0.00
NIN	4.64	0.00
BUB3	4.81	0.00
TUBD1	5.16	0.00
CEP120	5.17	0.00
C14orf80	5.28	0.00
ANKRD26	5.37	0.00
CEP78	5.50	0.00
CRADD	5.52	0.00
CEP44	5.54	0.00
ODF2	5.57	0.00
KIAA0753	5.68	0.00
CEP76	5.70	0.00
OFD1	5.82	0.00
C2CD3	6.04	0.00
CEP128	6.41	0.00
PIBF1	6.43	0.00
PIDD	6.50	0.00
CASP2	6.66	0.00
CCDC41	7.65	0.00

Screen	Correlation	Plot
Tozasertib 0.1μM R07 exp414	0.251	View
RO-3307 4.7μM R02 exp80	0.129	View
Staurosporine 0.02μM R08 exp524	0.078	View
Olaparib 4μM R08 exp512	0.072	View
MS023 2μM R03 exp134	0.067	View
MS023 7μM R03 exp135	0.065	View
ML-792 0.2μM R08 exp505	0.056	View
Pyridostatin 4μM R05 exp240	0.054	View
Mitomycin-C 0.06μM R08 exp503	0.054	View
Trimetrexate 0.03μM R08 exp535	0.053	View
Cytochalasin-B 5μM R07 exp349	0.273	View
Hippuristanol 0.12μM R08 exp489 no dilution day6	0.076	View
Centrinone 0.5μM R07 exp343	0.071	View
Ethanol 0.01 R05 exp234	0.069	View
Hippuristanol 0.12μM R08 exp488	0.069	View
GSK626616 14μM R08 exp485	0.068	View
CR131-b 0.005μM R08 exp474	0.061	View
AICAR 240μM R05 exp211	0.061	View
Doxorubicin 0.02μM R08 exp478	0.059	View
Aphidicolin 0.4μM R08 exp440	0.058	View
CB-5083 0.4μM R08 exp468	0.055	View
Etoposide 0.3μM R05 exp199	0.054	View
AICAR 240μM R03 exp116	0.053	View
Benzoate 20000μM R08 exp456	0.052	View

GO Term	Fold Change	Genes
pre-mRNA binding	54.18	SRSF6,EP300,HNRNPA2B1
internal protein amino acid acetylation	49.79	TAF5L,EP300,BRD8
peptidyl-lysine acetylation	48.48	TAF5L,EP300,BRD8
histone modification	36.99	TAF5L,EP300,DOT1L,BRD8,HDAC1
protein-DNA complex organization	6.27	H2AZ2,PHF1,EP300,IRF4,DOT1L,GPX4,BRD8,HDAC1

GO Term	Fold Change	Genes
ciliary transition fiber	236.84	CEP83,NIN,SCLT1
centriolar subdistal appendage	184.21	CEP128,ODF2,NIN
protein localization to centrosome	100.48	CEP83,PIBF1,C2CD3,KIAA0753
centriole replication	92.11	C2CD3,OFD1,KIAA0753,CEP44
centriole assembly	71.31	C2CD3,OFD1,KIAA0753,CEP44
centrosome duplication	63.16	C2CD3,OFD1,KIAA0753,CEP44
protein localization to microtubule cytoskeleton	59.74	CEP83,PIBF1,C2CD3,KIAA0753
centriole	52.63	CEP83,CEP128,C2CD3,OFD1,CEP76,KIAA0753,ODF2,CEP44,CEP78,CEP120,TUBD1,NIN,CEP20,SCLT1
centrosome cycle	49.12	C2CD3,OFD1,KIAA0753,ODF2,CEP44,CEP120,NIN,TUBE1
microtubule organizing center organization	41.32	C2CD3,OFD1,KIAA0753,ODF2,CEP44,CEP120,NIN,TUBE1
cilium organization	15.40	CEP83,PIBF1,C2CD3,OFD1,KIAA0753,ODF2,CEP78,CEP20,SCLT1
centrosome	14.40	CEP83,PIBF1,CEP128,C2CD3,OFD1,CEP76,KIAA0753,ODF2,CEP44,CEP78,ANKRD26,CEP120,NIN,CEP20,TUBE1,SCLT1
cilium assembly	13.11	CEP83,PIBF1,C2CD3,OFD1,KIAA0753,CEP20,SCLT1
microtubule cytoskeleton organization	12.33	PIBF1,C2CD3,OFD1,KIAA0753,ODF2,CEP44,CEP120,TUBD1,NIN,CEP20,TUBE1,INTS13
microtubule organizing center	11.67	CEP83,PIBF1,CEP128,C2CD3,OFD1,CEP76,KIAA0753,ODF2,CEP44,CEP78,ANKRD26,CEP120,TUBD1,NIN,CEP20,TUBE1,SCLT1
cell cycle process	8.87	CASP2,PIDD1,PIBF1,C2CD3,OFD1,KIAA0753,ODF2,CEP44,CRADD,CEP120,BUB3,NIN,TUBE1,INTS13
microtubule-based process	8.51	PIBF1,C2CD3,OFD1,KIAA0753,ODF2,CEP44,CEP120,TUBD1,NIN,CEP20,TUBE1,INTS13
mitotic cell cycle	8.29	CASP2,PIDD1,PIBF1,OFD1,CRADD,TUBD1,BUB3,TUBE1,INTS13
cilium	8.14	CEP83,CEP128,C2CD3,OFD1,ODF2,CEP78,TUBD1,NIN,CEP20,SCLT1

Latrunculin-B 10μM R07 exp374

Mechanism of Action

Technical Notes

Compound References

Compound Supplier

Compound Characterization

Dose Response Curve

Screen Summary

Screen Results