2-Methoxyestradiol 0.2μM R01 exp40 [The Human Chemical-Genetic Interaction Map Project]

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====== 2-Methoxyestradiol 0.2μM R01 exp40 ======
==== Mechanism of Action ====
Endogenous estradiol metabolite, weak agonist for estrogen receptor, agonist of GPER1, microtubule destabilizer
  * **<color #00a2e8>Class / Subclass 1:</color>** Metabolism / Metabolite
  * **<color #00a2e8>Class / Subclass 2:</color>** Signal Transduction / GPCR Agonist
  * **<color #00a2e8>Class / Subclass 3:</color>** Cell Cycle / Microtubule Poison


==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%2-Methoxyestradiol%%
    * **<color #00a2e8>Synonyms:</color>** 2-ME2; NSC-659853
    * **<color #00a2e8>CAS #:</color>** 362-07-2
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/66414|66414]]
    * **<color #00a2e8>IUPAC:</color>** %%(8R,9S,13S,14S,17S)-2-methoxy-13-methyl-6,7,8,9,11,12,14,15,16,17-decahydrocyclopenta[a]phenanthrene-3,17-diol%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C19H26O3/c1-19-8-7-12-13(15(19)5-6-18(19)21)4-3-11-9-16(20)17(22-2)10-14(11)12/h9-10,12-13,15,18,20-21H,3-8H2,1-2H3/t12-,13+,15-,18-,19-/m0/s1
    * **<color #00a2e8>INCHI Key:</color>** CQOQDQWUFQDJMK-SSTWWWIQSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 302.4
    * **<color #00a2e8>Canonical SMILES:</color>** CC12CCC3C(C1CCC2O)CCC4=CC(=C(C=C34)OC)O
    * **<color #00a2e8>Isomeric SMILES:</color>** C[C@]12CC[C@H]3[C@H]([C@@H]1CC[C@@H]2O)CCC4=CC(=C(C=C34)OC)O
    * **<color #00a2e8>Molecular Formula:</color>** C19H26O3
{{:chemogenomics:structures:chem-0021.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Toronto Research Chemicals
    * **<color #00a2e8>Catalog #:</color>** M262625
    * **<color #00a2e8>Lot #:</color>** 1-GNM-86-4
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+H)+ Calcd for C19H26O3 303.19547; found 303.19491
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** 2-METHOXYESTRADIOL
    * **<color #00a2e8>Min:</color>** -13.1327; **<color #00a2e8>Max:</color>** 72.1806
{{:chemogenomics:dose_response:dr_59.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |0.5415    |
| IC30  |0.9352    |
| IC40  |1.4635    |
| IC50  |2.2073    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 01
    * **<color #00a2e8>Dose</color>**: 200nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 7.0
    * **<color #00a2e8>Numbers of reads</color>**: 13228670


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|0/0|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/2-Methoxyestradiol_0.2uM_Round-1_exp40.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="default" size="sm" modal="modal_go_sensitive">View</button>|<button type="default" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='2-Methoxyestradiol 0.2μM R01 exp40'>
{{:chemogenomics:cranks_plots:exp40.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:M:MRPL38|MRPL38]]|-2.73|0.73|
|[[:human_genes:M:MAGEB17|MAGEB17]]|-2.68|0.73|
|[[:human_genes:N:NSMCE1|NSMCE1]]|-2.61|0.73|
|[[:human_genes:R:RSRC2|RSRC2]]|-2.60|0.73|
|[[:human_genes:C:CAMLG|CAMLG]]|-2.38|0.73|
|[[:human_genes:E:ERCC2|ERCC2]]|-2.38|0.73|
|[[:human_genes:G:GKAP1|GKAP1]]|-2.29|0.83|
|[[:human_genes:C:CD28|CD28]]|-2.28|0.73|
|[[:human_genes:Z:ZNF512|ZNF512]]|-2.27|0.73|
|[[:human_genes:G:GPC4|GPC4]]|-2.23|0.73|
|[[:human_genes:H:HEPN1|HEPN1]]|-2.23|0.73|
|[[:human_genes:T:TIMM21|TIMM21]]|-2.22|0.73|
|[[:human_genes:T:TOP1|TOP1]]|-2.22|0.73|
|[[:human_genes:G:GAS1|GAS1]]|-2.20|0.73|
|[[:human_genes:D:DGKB|DGKB]]|-2.19|0.73|
|[[:human_genes:M:MYO3A|MYO3A]]|-2.14|0.73|
|[[:human_genes:P:PAXIP1|PAXIP1]]|-2.13|0.73|
|[[:human_genes:T:TBCD|TBCD]]|-2.13|0.73|
|[[:human_genes:D:DHX33|DHX33]]|-2.10|0.73|
|[[:human_genes:P:PPP1CB|PPP1CB]]|-2.09|0.83|
|[[:human_genes:C:C5orf49|C5orf49]]|-2.09|0.73|
|[[:human_genes:N:NARS|NARS]]|-2.09|0.74|
|[[:human_genes:U:UHRF1|UHRF1]]|-2.08|0.73|
|[[:human_genes:S:SLC22A14|SLC22A14]]|-2.08|0.73|
|[[:human_genes:A:AHCY|AHCY]]|-2.08|0.73|
|[[:human_genes:C:CHGB|CHGB]]|-2.08|0.74|
|[[:human_genes:U:URI1|URI1]]|-2.06|0.73|
|[[:human_genes:U:USP16|USP16]]|-2.06|0.73|
|[[:human_genes:G:GMPPB|GMPPB]]|-2.06|0.73|
|[[:human_genes:C:CDC20B|CDC20B]]|-2.05|0.76|
|[[:human_genes:M:MARVELD1|MARVELD1]]|2.13|0.74|
|[[:human_genes:T:TRIM26|TRIM26]]|2.13|0.68|
|[[:human_genes:R:RFC1|RFC1]]|2.15|0.69|
|[[:human_genes:P:PSMD14|PSMD14]]|2.15|0.69|
|[[:human_genes:R:RPL27|RPL27]]|2.16|0.69|
|[[:human_genes:A:ACMSD|ACMSD]]|2.16|0.67|
|[[:human_genes:C:CCDC109B|CCDC109B]]|2.17|0.67|
|[[:human_genes:P:POLR2K|POLR2K]]|2.19|0.69|
|[[:human_genes:P:PSMB4|PSMB4]]|2.23|0.67|
|[[:human_genes:Z:ZNF431|ZNF431]]|2.23|0.69|
|[[:human_genes:E:ERI2|ERI2]]|2.24|0.67|
|[[:human_genes:R:RPA3|RPA3]]|2.24|0.67|
|[[:human_genes:H:HINFP|HINFP]]|2.26|0.67|
|[[:human_genes:N:NPHS2|NPHS2]]|2.26|0.67|
|[[:human_genes:P:POLE|POLE]]|2.30|0.67|
|[[:human_genes:G:GPRIN2|GPRIN2]]|2.30|0.67|
|[[:human_genes:R:RBM39|RBM39]]|2.32|0.68|
|[[:human_genes:R:RUVBL2|RUVBL2]]|2.34|0.69|
|[[:human_genes:T:TMEM251|TMEM251]]|2.36|0.68|
|[[:human_genes:A:ACO2|ACO2]]|2.38|0.69|
|[[:human_genes:T:TTK|TTK]]|2.38|0.68|
|[[:human_genes:B:B3GNT4|B3GNT4]]|2.39|0.67|
|[[:human_genes:A:ASB8|ASB8]]|2.39|0.68|
|[[:human_genes:C:CPM|CPM]]|2.41|0.67|
|[[:human_genes:U:UBA52|UBA52]]|2.44|0.69|
|[[:human_genes:N:NOP2|NOP2]]|2.48|0.67|
|[[:human_genes:A:ATP6AP2|ATP6AP2]]|2.50|0.67|
|[[:human_genes:S:SUPT6H|SUPT6H]]|2.54|0.68|
|[[:human_genes:P:PAF1|PAF1]]|2.76|0.67|
|[[:human_genes:T:TICRR|TICRR]]|2.77|0.67|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp49|NFN1 0.1μM R01 exp49]]|0.085|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp53|Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53]]|0.052|<button type="primary" size="small" modal="2">View</button>|
</modal>


<modal id="1" size="lg" title="2-Methoxyestradiol 0.2μM R01 exp40  vs  NFN1 0.1μM R01 exp49">
{{:chemogenomics:comparison_plots:exp40_vs_exp49.png?nolink |}}
</modal>

<modal id="2" size="lg" title="2-Methoxyestradiol 0.2μM R01 exp40  vs  Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53">
{{:chemogenomics:comparison_plots:exp40_vs_exp53.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">No GO term hits below threshold. (FDR <0.05)</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">No GO term hits below threshold. (FDR <0.05)</modal>