2-Methoxyestradiol 0.2μM R01 exp40

Mechanism of Action

Endogenous estradiol metabolite, weak agonist for estrogen receptor, agonist of GPER1, microtubule destabilizer

  • Class / Subclass 1: Metabolism / Metabolite
  • Class / Subclass 2: Signal Transduction / GPCR Agonist
  • Class / Subclass 3: Cell Cycle / Microtubule Poison

Technical Notes

Compound References

  • PubChem Name: 2-Methoxyestradiol
  • Synonyms: 2-ME2; NSC-659853
  • CAS #: 362-07-2
  • PubChem CID: 66414
  • IUPAC: (8R,9S,13S,14S,17S)-2-methoxy-13-methyl-6,7,8,9,11,12,14,15,16,17-decahydrocyclopenta[a]phenanthrene-3,17-diol
  • INCHI Name: InChI=1S/C19H26O3/c1-19-8-7-12-13(15(19)5-6-18(19)21)4-3-11-9-16(20)17(22-2)10-14(11)12/h9-10,12-13,15,18,20-21H,3-8H2,1-2H3/t12-,13+,15-,18-,19-/m0/s1
  • INCHI Key: CQOQDQWUFQDJMK-SSTWWWIQSA-N
  • Molecular Weight: 302.4
  • Canonical SMILES: CC12CCC3C(C1CCC2O)CCC4=CC(=C(C=C34)OC)O
  • Isomeric SMILES: C[C@]12CC[C@H]3[C@H]([C@@H]1CC[C@@H]2O)CCC4=CC(=C(C=C34)OC)O
  • Molecular Formula: C19H26O3

Compound Supplier

  • Supplier Name: Toronto Research Chemicals
  • Catalog #: M262625
  • Lot #: 1-GNM-86-4

Compound Characterization

  • HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C19H26O3 303.19547; found 303.19491

Dose Response Curve

  • Platform ID: 2-METHOXYESTRADIOL
  • Min: -13.1327; Max: 72.1806





IC Concentration (µM)
IC10 N/A
IC20 0.5415
IC30 0.9352
IC40 1.4635
IC50 2.2073
IC60 N/A
IC70 N/A
IC80 N/A
IC90 N/A


Screen Summary

  • Round: 01
  • Dose: 200nM
  • Days of incubation: 8
  • Doublings: 7.0
  • Numbers of reads: 13228670

Screen Results

Sensitive/Resistant hits (FDR<0.05)CRANKSScore PlotTop 30 GenesScreen SimilarityTop 30 Sensitive GO termsTop 30 Resistant GO terms
0/0ScoresViewViewViewViewView

  • Last modified: 2026/01/15 21:36
  • by 127.0.0.1