Bafilomycin-A1 0.009μM R08 exp454 [The Human Chemical-Genetic Interaction Map Project]

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====== Bafilomycin-A1 0.009μM R08 exp454 ======
==== Mechanism of Action ====
Inhibits vacuolar-type H+ ATPase, blocks acidification of lysosome
  * **<color #00a2e8>Class / Subclass 1:</color>** Signal Transduction / Ion Channel Inhibitor
  * **<color #00a2e8>Class / Subclass 2:</color>** Organelle Function / Membrane Transport Inhibitor
  * **<color #00a2e8>Class / Subclass 3:</color>** Proteostasis / Autophagy Inhibitor


==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Bafilomycin A1%%
    * **<color #00a2e8>Synonyms:</color>** (-)-Bafilomycin A1
    * **<color #00a2e8>CAS #:</color>** 88899-55-2
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/6436223|6436223]]
    * **<color #00a2e8>IUPAC:</color>** %%(3Z,5E,7R,8S,9S,11E,13E,15S,16R)-16-[(2S,3R,4S)-4-[(2R,4R,5S,6R)-2,4-dihydroxy-5-methyl-6-propan-2-yloxan-2-yl]-3-hydroxypentan-2-yl]-8-hydroxy-3,15-dimethoxy-5,7,9,11-tetramethyl-1-oxacyclohexadeca-3,5,11,13-tetraen-2-one%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C35H58O9/c1-19(2)32-24(7)27(36)18-35(40,44-32)26(9)31(38)25(8)33-28(41-10)14-12-13-20(3)15-22(5)30(37)23(6)16-21(4)17-29(42-11)34(39)43-33/h12-14,16-17,19,22-28,30-33,36-38,40H,15,18H2,1-11H3/b14-12+,20-13+,21-16+,29-17-/t22-,23+,24-,25-,26-,27+,28-,30-,31+,32+,33+,35+/m0/s1
    * **<color #00a2e8>INCHI Key:</color>** XDHNQDDQEHDUTM-JQWOJBOSSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 622.8
    * **<color #00a2e8>Canonical SMILES:</color>** CC1CC(=CC=CC(C(OC(=O)C(=CC(=CC(C1O)C)C)OC)C(C)C(C(C)C2(CC(C(C(O2)C(C)C)C)O)O)O)OC)C
    * **<color #00a2e8>Isomeric SMILES:</color>** C[C@H]1C/C(=C/C=C/[C@@H]([C@H](OC(=O)/C(=C/C(=C/[C@H]([C@H]1O)C)/C)/OC)[C@@H](C)[C@H]([C@H](C)[C@]2(C[C@H]([C@@H]([C@H](O2)C(C)C)C)O)O)O)OC)/C
    * **<color #00a2e8>Molecular Formula:</color>** C35H58O9
{{:chemogenomics:structures:chem-0265.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Med Chem Express
    * **<color #00a2e8>Catalog #:</color>** HY-100558
    * **<color #00a2e8>Lot #:</color>** 29352
</panel>


<panel title='Compound Characterization' collapsed='true'>
    * **<color #00a2e8>HRMS (ESI-TOF) m/z:</color>** (M+Na)+ Calcd for C35H58O9 645.3973; found 645.39975
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Bafilomycin_A1
    * **<color #00a2e8>Min:</color>** -10.6253; **<color #00a2e8>Max:</color>** 97.1608
{{:chemogenomics:dose_response:dr_502.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |0.0065    |
| IC20  |0.0090    |
| IC30  |0.0111    |
| IC40  |0.0132    |
| IC50  |0.0154    |
| IC60  |0.0181    |
| IC70  |0.0215    |
| IC80  |0.0265    |
| IC90  |0.0364    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 08
    * **<color #00a2e8>Dose</color>**: 9nM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 5.3
    * **<color #00a2e8>Numbers of reads</color>**: 14931515


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|76/21|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Bafilomycin_A1_0.009uM_Round-8_exp454.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Bafilomycin-A1 0.009μM R08 exp454'>
{{:chemogenomics:cranks_plots:exp454.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:G:GPR107|GPR107]]|-4.98|0.00|
|[[:human_genes:A:ATP6V0A2|ATP6V0A2]]|-4.63|0.00|
|[[:human_genes:S:SCYL1|SCYL1]]|-4.25|0.00|
|[[:human_genes:U:UNC50|UNC50]]|-4.25|0.00|
|[[:human_genes:S:SLC5A3|SLC5A3]]|-3.95|0.00|
|[[:human_genes:D:DAGLB|DAGLB]]|-3.74|0.00|
|[[:human_genes:S:SLC39A9|SLC39A9]]|-3.60|0.00|
|[[:human_genes:S:SLC25A1|SLC25A1]]|-3.47|0.00|
|[[:human_genes:D:DMXL1|DMXL1]]|-3.28|0.00|
|[[:human_genes:E:ENTPD4|ENTPD4]]|-3.26|0.00|
|[[:human_genes:T:TSSC1|TSSC1]]|-3.17|0.00|
|[[:human_genes:T:TBC1D22B|TBC1D22B]]|-3.12|0.00|
|[[:human_genes:V:VKORC1L1|VKORC1L1]]|-3.11|0.00|
|[[:human_genes:A:ACSL4|ACSL4]]|-3.04|<0.01|
|[[:human_genes:A:ARL1|ARL1]]|-3.03|<0.01|
|[[:human_genes:K:KIAA1033|KIAA1033]]|-3.02|<0.01|
|[[:human_genes:Y:YIPF6|YIPF6]]|-2.92|<0.01|
|[[:human_genes:Z:ZDHHC6|ZDHHC6]]|-2.92|<0.01|
|[[:human_genes:S:SCAP|SCAP]]|-2.89|<0.01|
|[[:human_genes:R:RSRC2|RSRC2]]|-2.86|0.09|
|[[:human_genes:V:VPS29|VPS29]]|-2.84|<0.01|
|[[:human_genes:N:NOSIP|NOSIP]]|-2.76|<0.01|
|[[:human_genes:M:MMD|MMD]]|-2.69|<0.01|
|[[:human_genes:C:CCDC22|CCDC22]]|-2.69|<0.01|
|[[:human_genes:R:RAB1B|RAB1B]]|-2.69|<0.01|
|[[:human_genes:C:COMMD10|COMMD10]]|-2.68|<0.01|
|[[:human_genes:R:RALGAPB|RALGAPB]]|-2.68|<0.01|
|[[:human_genes:V:VAC14|VAC14]]|-2.65|<0.01|
|[[:human_genes:C:COMMD7|COMMD7]]|-2.64|<0.01|
|[[:human_genes:P:PEX7|PEX7]]|-2.63|<0.01|
|[[:human_genes:C:CHD7|CHD7]]|2.26|0.09|
|[[:human_genes:S:SFXN1|SFXN1]]|2.27|0.06|
|[[:human_genes:G:GFM2|GFM2]]|2.27|0.06|
|[[:human_genes:I:ICMT|ICMT]]|2.29|0.06|
|[[:human_genes:N:NDC80|NDC80]]|2.30|0.23|
|[[:human_genes:S:SHMT2|SHMT2]]|2.30|0.11|
|[[:human_genes:R:RAPGEF4|RAPGEF4]]|2.34|0.05|
|[[:human_genes:D:DTNBP1|DTNBP1]]|2.35|0.05|
|[[:human_genes:Z:ZBTB33|ZBTB33]]|2.35|0.05|
|[[:human_genes:M:MON1B|MON1B]]|2.36|0.07|
|[[:human_genes:N:NDUFAF6|NDUFAF6]]|2.38|0.04|
|[[:human_genes:R:RMND1|RMND1]]|2.42|0.04|
|[[:human_genes:S:SEC23B|SEC23B]]|2.43|0.06|
|[[:human_genes:N:NDUFA2|NDUFA2]]|2.45|0.05|
|[[:human_genes:S:SEC31A|SEC31A]]|2.46|0.03|
|[[:human_genes:A:AK3|AK3]]|2.50|0.03|
|[[:human_genes:M:MAGT1|MAGT1]]|2.50|0.03|
|[[:human_genes:F:FAM122A|FAM122A]]|2.51|0.03|
|[[:human_genes:S:SRM|SRM]]|2.53|0.03|
|[[:human_genes:E:EIF4G3|EIF4G3]]|2.56|0.04|
|[[:human_genes:A:AP3D1|AP3D1]]|2.65|0.02|
|[[:human_genes:T:TMEM120B|TMEM120B]]|2.66|0.03|
|[[:human_genes:L:LDLRAP1|LDLRAP1]]|2.70|0.03|
|[[:human_genes:A:AP3M1|AP3M1]]|2.74|0.02|
|[[:human_genes:R:RAB8A|RAB8A]]|2.76|0.02|
|[[:human_genes:A:ATP2B1|ATP2B1]]|2.81|0.02|
|[[:human_genes:A:ACACA|ACACA]]|2.82|0.02|
|[[:human_genes:H:HERC3|HERC3]]|2.89|0.01|
|[[:human_genes:C:CDK2|CDK2]]|3.08|<0.01|
|[[:human_genes:R:RABL6|RABL6]]|3.78|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp68|Clomiphene 4.4μM R02 exp68]]|0.177|<button type="primary" size="small" modal="1">View</button>|
|[[:results:exp89|Vemurafenib 6.6μM R02 exp89]]|0.117|<button type="primary" size="small" modal="2">View</button>|
|[[:results:exp434|Vemurafenib 6.6μM R08 exp434]]|0.115|<button type="primary" size="small" modal="3">View</button>|
|[[:results:exp470|Chloroquine 32μM R08 exp470]]|0.088|<button type="primary" size="small" modal="4">View</button>|
|[[:results:exp227|Cryptotanshinone 12μM R05 exp227]]|0.086|<button type="primary" size="small" modal="5">View</button>|
|[[:results:exp483|FTY720 3μM R08 exp483]]|0.083|<button type="primary" size="small" modal="6">View</button>|
|[[:results:exp530|Thioridazine 5μM R08 exp530]]|0.076|<button type="primary" size="small" modal="7">View</button>|
|[[:results:exp79|Q15 2.7μM R02 exp79]]|0.072|<button type="primary" size="small" modal="8">View</button>|
|[[:results:exp164|Q15 1 to 2μM on day4 R04 exp164]]|0.071|<button type="primary" size="small" modal="9">View</button>|
|[[:results:exp510|Nicotine 3000μM R08 exp510]]|0.063|<button type="primary" size="small" modal="10">View</button>|
|[[:results:exp346|CoCl2 18μM R07 exp346]]|0.063|<button type="primary" size="small" modal="11">View</button>|
|[[:results:exp416|Tubacin 1.6μM R07 exp416]]|0.055|<button type="primary" size="small" modal="12">View</button>|
|[[:results:exp344|Chlorpromazine 10μM R07 exp344]]|0.053|<button type="primary" size="small" modal="13">View</button>|
|[[:results:exp490|Hydroxychloroquine 30μM R08 exp490]]|0.053|<button type="primary" size="small" modal="14">View</button>|
|[[:results:exp282|Fluvastatin 2.2μM R06 exp282]]|0.051|<button type="primary" size="small" modal="15">View</button>|
|[[:results:exp169|BH1 1μM R04 exp169]]|0.121|<button type="primary" size="small" modal="16">View</button>|
|[[:results:exp461|BS-181 20μM R08 exp461]]|0.089|<button type="primary" size="small" modal="17">View</button>|
|[[:results:exp321|ABT-702 5μM plus Deferoxamine 11μM R07 exp321]]|0.053|<button type="primary" size="small" modal="18">View</button>|
</modal>


<modal id="1" size="lg" title="Bafilomycin-A1 0.009μM R08 exp454  vs  Clomiphene 4.4μM R02 exp68">
{{:chemogenomics:comparison_plots:exp454_vs_exp68.png?nolink |}}
</modal>

<modal id="2" size="lg" title="Bafilomycin-A1 0.009μM R08 exp454  vs  Vemurafenib 6.6μM R02 exp89">
{{:chemogenomics:comparison_plots:exp454_vs_exp89.png?nolink |}}
</modal>

<modal id="3" size="lg" title="Bafilomycin-A1 0.009μM R08 exp454  vs  Vemurafenib 6.6μM R08 exp434">
{{:chemogenomics:comparison_plots:exp434_vs_exp454.png?nolink |}}
</modal>

<modal id="4" size="lg" title="Bafilomycin-A1 0.009μM R08 exp454  vs  Chloroquine 32μM R08 exp470">
{{:chemogenomics:comparison_plots:exp454_vs_exp470.png?nolink |}}
</modal>

<modal id="5" size="lg" title="Bafilomycin-A1 0.009μM R08 exp454  vs  Cryptotanshinone 12μM R05 exp227">
{{:chemogenomics:comparison_plots:exp227_vs_exp454.png?nolink |}}
</modal>

<modal id="6" size="lg" title="Bafilomycin-A1 0.009μM R08 exp454  vs  FTY720 3μM R08 exp483">
{{:chemogenomics:comparison_plots:exp454_vs_exp483.png?nolink |}}
</modal>

<modal id="7" size="lg" title="Bafilomycin-A1 0.009μM R08 exp454  vs  Thioridazine 5μM R08 exp530">
{{:chemogenomics:comparison_plots:exp454_vs_exp530.png?nolink |}}
</modal>

<modal id="8" size="lg" title="Bafilomycin-A1 0.009μM R08 exp454  vs  Q15 2.7μM R02 exp79">
{{:chemogenomics:comparison_plots:exp454_vs_exp79.png?nolink |}}
</modal>

<modal id="9" size="lg" title="Bafilomycin-A1 0.009μM R08 exp454  vs  Q15 1 to 2μM on day4 R04 exp164">
{{:chemogenomics:comparison_plots:exp164_vs_exp454.png?nolink |}}
</modal>

<modal id="10" size="lg" title="Bafilomycin-A1 0.009μM R08 exp454  vs  Nicotine 3000μM R08 exp510">
{{:chemogenomics:comparison_plots:exp454_vs_exp510.png?nolink |}}
</modal>

<modal id="11" size="lg" title="Bafilomycin-A1 0.009μM R08 exp454  vs  CoCl2 18μM R07 exp346">
{{:chemogenomics:comparison_plots:exp346_vs_exp454.png?nolink |}}
</modal>

<modal id="12" size="lg" title="Bafilomycin-A1 0.009μM R08 exp454  vs  Tubacin 1.6μM R07 exp416">
{{:chemogenomics:comparison_plots:exp416_vs_exp454.png?nolink |}}
</modal>

<modal id="13" size="lg" title="Bafilomycin-A1 0.009μM R08 exp454  vs  Chlorpromazine 10μM R07 exp344">
{{:chemogenomics:comparison_plots:exp344_vs_exp454.png?nolink |}}
</modal>

<modal id="14" size="lg" title="Bafilomycin-A1 0.009μM R08 exp454  vs  Hydroxychloroquine 30μM R08 exp490">
{{:chemogenomics:comparison_plots:exp454_vs_exp490.png?nolink |}}
</modal>

<modal id="15" size="lg" title="Bafilomycin-A1 0.009μM R08 exp454  vs  Fluvastatin 2.2μM R06 exp282">
{{:chemogenomics:comparison_plots:exp282_vs_exp454.png?nolink |}}
</modal>

<modal id="16" size="lg" title="BH1 1μM R04 exp169  vs  Bafilomycin-A1 0.009μM R08 exp454">
{{:chemogenomics:comparison_plots:exp169_vs_exp454.png?nolink |}}
</modal>

<modal id="17" size="lg" title="BS-181 20μM R08 exp461  vs  Bafilomycin-A1 0.009μM R08 exp454">
{{:chemogenomics:comparison_plots:exp454_vs_exp461.png?nolink |}}
</modal>

<modal id="18" size="lg" title="ABT-702 5μM plus Deferoxamine 11μM R07 exp321  vs  Bafilomycin-A1 0.009μM R08 exp454">
{{:chemogenomics:comparison_plots:exp321_vs_exp454.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|Golgi membrane|8.42|ATP6V0A2,UNC50,ENTPD4,ARL1,YIPF6,SCAP,NOSIP,RAB1B,VAC14|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|synaptic vesicle cytoskeletal transport|110.46|AP3M1,AP3D1,DTNBP1|
|synaptic vesicle transport along microtubule|110.46|AP3M1,AP3D1,DTNBP1|
|anterograde synaptic vesicle transport|110.46|AP3M1,AP3D1,DTNBP1|
|vesicle cargo loading|71.26|AP3M1,AP3D1,SEC31A,SEC23B|
|platelet dense granule organization|69.04|AP3M1,AP3D1,DTNBP1|
|membrane coat|24.82|AP3M1,AP3D1,SEC31A,SEC23B|
|vesicle organization|10.13|RAB8A,AP3M1,AP3D1,SEC31A,SEC23B,DTNBP1|
</modal>