Bafilomycin-A1 0.009μM R08 exp454
Mechanism of Action
Inhibits vacuolar-type H+ ATPase, blocks acidification of lysosome
- Class / Subclass 1: Signal Transduction / Ion Channel Inhibitor
- Class / Subclass 2: Organelle Function / Membrane Transport Inhibitor
- Class / Subclass 3: Proteostasis / Autophagy Inhibitor
Technical Notes
Compound References
- PubChem Name: Bafilomycin A1
- Synonyms: (-)-Bafilomycin A1
- CAS #: 88899-55-2
- PubChem CID: 6436223
- IUPAC: (3Z,5E,7R,8S,9S,11E,13E,15S,16R)-16-[(2S,3R,4S)-4-[(2R,4R,5S,6R)-2,4-dihydroxy-5-methyl-6-propan-2-yloxan-2-yl]-3-hydroxypentan-2-yl]-8-hydroxy-3,15-dimethoxy-5,7,9,11-tetramethyl-1-oxacyclohexadeca-3,5,11,13-tetraen-2-one
- INCHI Name: InChI=1S/C35H58O9/c1-19(2)32-24(7)27(36)18-35(40,44-32)26(9)31(38)25(8)33-28(41-10)14-12-13-20(3)15-22(5)30(37)23(6)16-21(4)17-29(42-11)34(39)43-33/h12-14,16-17,19,22-28,30-33,36-38,40H,15,18H2,1-11H3/b14-12+,20-13+,21-16+,29-17-/t22-,23+,24-,25-,26-,27+,28-,30-,31+,32+,33+,35+/m0/s1
- INCHI Key: XDHNQDDQEHDUTM-JQWOJBOSSA-N
- Molecular Weight: 622.8
- Canonical SMILES: CC1CC(=CC=CC(C(OC(=O)C(=CC(=CC(C1O)C)C)OC)C(C)C(C(C)C2(CC(C(C(O2)C(C)C)C)O)O)O)OC)C
- Isomeric SMILES: C[C@H]1C/C(=C/C=C/[C@@H]([C@H](OC(=O)/C(=C/C(=C/[C@H]([C@H]1O)C)/C)/OC)[C@@H](C)[C@H]([C@H](C)[C@]2(C[C@H]([C@@H]([C@H](O2)C(C)C)C)O)O)O)OC)/C
- Molecular Formula: C35H58O9
Compound Supplier
- Supplier Name: Med Chem Express
- Catalog #: HY-100558
- Lot #: 29352
Compound Characterization
- HRMS (ESI-TOF) m/z: (M+Na)+ Calcd for C35H58O9 645.3973; found 645.39975
Dose Response Curve
- Platform ID: Bafilomycin_A1
- Min: -10.6253; Max: 97.1608
| IC | Concentration (µM) |
|---|---|
| IC10 | 0.0065 |
| IC20 | 0.0090 |
| IC30 | 0.0111 |
| IC40 | 0.0132 |
| IC50 | 0.0154 |
| IC60 | 0.0181 |
| IC70 | 0.0215 |
| IC80 | 0.0265 |
| IC90 | 0.0364 |
Screen Summary
- Round: 08
- Dose: 9nM
- Days of incubation: 8
- Doublings: 5.3
- Numbers of reads: 14931515
Screen Results
| Sensitive/Resistant hits (FDR<0.05) | CRANKS | Score Plot | Top 30 Genes | Screen Similarity | Top 30 Sensitive GO terms | Top 30 Resistant GO terms |
|---|---|---|---|---|---|---|
| 76/21 | Scores |
Bafilomycin-A1 0.009μM R08 exp454
Bafilomycin-A1 0.009μM R08 exp454 vs Clomiphene 4.4μM R02 exp68
Bafilomycin-A1 0.009μM R08 exp454 vs Vemurafenib 6.6μM R02 exp89
Bafilomycin-A1 0.009μM R08 exp454 vs Vemurafenib 6.6μM R08 exp434
Bafilomycin-A1 0.009μM R08 exp454 vs Chloroquine 32μM R08 exp470
Bafilomycin-A1 0.009μM R08 exp454 vs Cryptotanshinone 12μM R05 exp227
Bafilomycin-A1 0.009μM R08 exp454 vs FTY720 3μM R08 exp483
Bafilomycin-A1 0.009μM R08 exp454 vs Thioridazine 5μM R08 exp530
Bafilomycin-A1 0.009μM R08 exp454 vs Q15 2.7μM R02 exp79
Bafilomycin-A1 0.009μM R08 exp454 vs Q15 1 to 2μM on day4 R04 exp164
Bafilomycin-A1 0.009μM R08 exp454 vs Nicotine 3000μM R08 exp510
Bafilomycin-A1 0.009μM R08 exp454 vs CoCl2 18μM R07 exp346
Bafilomycin-A1 0.009μM R08 exp454 vs Tubacin 1.6μM R07 exp416
Bafilomycin-A1 0.009μM R08 exp454 vs Chlorpromazine 10μM R07 exp344
Bafilomycin-A1 0.009μM R08 exp454 vs Hydroxychloroquine 30μM R08 exp490
Bafilomycin-A1 0.009μM R08 exp454 vs Fluvastatin 2.2μM R06 exp282
BH1 1μM R04 exp169 vs Bafilomycin-A1 0.009μM R08 exp454
BS-181 20μM R08 exp461 vs Bafilomycin-A1 0.009μM R08 exp454
ABT-702 5μM plus Deferoxamine 11μM R07 exp321 vs Bafilomycin-A1 0.009μM R08 exp454
