Ribavirin 10μM R01 exp52 [The Human Chemical-Genetic Interaction Map Project]

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====== Ribavirin 10μM R01 exp52 ======
==== Mechanism of Action ====
Prodrug metabolized to purine RNA nucleotide mimetic, also inhibits EIF4E
  * **<color #00a2e8>Class / Subclass 1:</color>** Infectious Disease / Antiviral
  * **<color #00a2e8>Class / Subclass 2:</color>** Metabolism / Antimetabolite
  * **<color #00a2e8>Class / Subclass 3:</color>** DNA Damage, Repair and Replication / Reverse Transcriptase Inhibitor


==== Technical Notes ====
<accordion collapsed='true'>
<panel title='Compound References' collapsed='true'>
    * **<color #00a2e8>PubChem Name:</color>** %%Ribavirin%%
    * **<color #00a2e8>Synonyms:</color>** ICN-1229
    * **<color #00a2e8>CAS #:</color>** 36791-04-5
    * **<color #00a2e8>PubChem CID:</color>** [[https://pubchem.ncbi.nlm.nih.gov/compound/37542|37542]]
    * **<color #00a2e8>IUPAC:</color>** %%1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-1,2,4-triazole-3-carboxamide%%
    * **<color #00a2e8>INCHI Name:</color>** InChI=1S/C8H12N4O5/c9-6(16)7-10-2-12(11-7)8-5(15)4(14)3(1-13)17-8/h2-5,8,13-15H,1H2,(H2,9,16)/t3-,4-,5-,8-/m1/s1
    * **<color #00a2e8>INCHI Key:</color>** IWUCXVSUMQZMFG-AFCXAGJDSA-N
    * **<color #00a2e8>Molecular Weight:</color>** 244.2
    * **<color #00a2e8>Canonical SMILES:</color>** C1=NC(=NN1C2C(C(C(O2)CO)O)O)C(=O)N
    * **<color #00a2e8>Isomeric SMILES:</color>** C1=NC(=NN1[C@H]2[C@@H]([C@@H]([C@H](O2)CO)O)O)C(=O)N
    * **<color #00a2e8>Molecular Formula:</color>** C8H12N4O5
{{:chemogenomics:structures:chem-0032.svg?nolink}}
</panel>


<panel title='Compound Supplier' collapsed='true'>
    * **<color #00a2e8>Supplier Name:</color>** Kemprotec Ltd
    * **<color #00a2e8>Catalog #:</color>** K-1104
    * **<color #00a2e8>Lot #:</color>** 170221
</panel>


<panel title='Compound Characterization' collapsed='true'>
Characterization data not available.
</panel>


<panel title='Dose Response Curve' collapsed='true'>
    * **<color #00a2e8>Platform ID:</color>** Ribavirin
    * **<color #00a2e8>Min:</color>** -21.0950; **<color #00a2e8>Max:</color>** 37.2497
{{:chemogenomics:dose_response:dr_117.png?nolink&500 |}}
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|< 300px 100px 200px >|
^ IC   ^ Concentration (µM) ^
| IC10  |N/A    |
| IC20  |30.6995    |
| IC30  |36.4591    |
| IC40  |N/A    |
| IC50  |N/A    |
| IC60  |N/A    |
| IC70  |N/A    |
| IC80  |N/A    |
| IC90  |N/A    |
\\ 
</panel>
</accordion>
==== Screen Summary ====
    * **<color #00a2e8>Round</color>**: 01
    * **<color #00a2e8>Dose</color>**: 10µM
    * **<color #00a2e8>Days of incubation</color>**: 8
    * **<color #00a2e8>Doublings</color>**: 6.5
    * **<color #00a2e8>Numbers of reads</color>**: 9955062


==== Screen Results ====


^Sensitive/Resistant hits (FDR<0.05)^CRANKS^Score Plot^Top 30 Genes^Screen Similarity^Top 30 Sensitive GO terms^Top 30 Resistant GO terms^
|3/1|<color #e85500>[[https://files.tyerslab.com/files/public/chemogenomics/cranks/Ribavirin_10uM_Round-1_exp52.txt|Scores]]</color>|<button type="primary" size="sm" modal="ScorePlot">View</button>|<button type="primary" size="sm" modal="modal_hits">View</button>|<button type="primary" size="sm" modal="modal_screen_similarity">View</button>|<button type="primary" size="sm" modal="modal_go_sensitive">View</button>|<button type="primary" size="sm" modal="modal_go_resistant">View</button>|
<modal id='ScorePlot' size='lg' title='Ribavirin 10μM R01 exp52'>
{{:chemogenomics:cranks_plots:exp52.png?nolink}}
</modal>


<modal id="modal_hits" size="sm" title="Top 30 S/R Genes">
^Gene^CRANKS Score^FDR^
|[[:human_genes:A:AMPD2|AMPD2]]|-3.77|<0.01|
|[[:human_genes:P:PRPS1|PRPS1]]|-3.20|<0.01|
|[[:human_genes:H:HPRT1|HPRT1]]|-3.15|0.02|
|[[:human_genes:E:ERLIN2|ERLIN2]]|-3.05|0.15|
|[[:human_genes:S:STT3A|STT3A]]|-3.04|0.23|
|[[:human_genes:T:TBCD|TBCD]]|-3.03|0.08|
|[[:human_genes:S:SPDYE6|SPDYE6]]|-2.63|0.15|
|[[:human_genes:C:CTSV|CTSV]]|-2.63|0.18|
|[[:human_genes:R:RPS4Y1|RPS4Y1]]|-2.57|0.10|
|[[:human_genes:G:GPN2|GPN2]]|-2.54|0.16|
|[[:human_genes:N:NRDE2|NRDE2]]|-2.52|0.51|
|[[:human_genes:N:NSMCE1|NSMCE1]]|-2.51|0.23|
|[[:human_genes:O:ORC5|ORC5]]|-2.49|0.08|
|[[:human_genes:O:OR13C8|OR13C8]]|-2.49|0.17|
|[[:human_genes:M:MAL2|MAL2]]|-2.46|0.54|
|[[:human_genes:I:IPO9|IPO9]]|-2.44|0.28|
|[[:human_genes:O:ORC3|ORC3]]|-2.36|0.16|
|[[:human_genes:D:DHX33|DHX33]]|-2.32|0.38|
|[[:human_genes:A:ARL2|ARL2]]|-2.30|0.18|
|[[:human_genes:H:HNRNPD|HNRNPD]]|-2.30|0.15|
|[[:human_genes:M:MYH4|MYH4]]|-2.28|0.51|
|[[:human_genes:E:ERCC2|ERCC2]]|-2.27|0.39|
|[[:human_genes:V:VEZT|VEZT]]|-2.25|0.19|
|[[:human_genes:A:AHCY|AHCY]]|-2.25|0.19|
|[[:human_genes:R:RGS13|RGS13]]|-2.22|0.16|
|[[:human_genes:F:FAM172A|FAM172A]]|-2.21|0.35|
|[[:human_genes:N:NBAS|NBAS]]|-2.20|0.23|
|[[:human_genes:B:BEX4|BEX4]]|-2.18|0.51|
|[[:human_genes:N:NEK3|NEK3]]|-2.17|0.18|
|[[:human_genes:C:CRYZL1|CRYZL1]]|-2.16|0.38|
|[[:human_genes:C:COA3|COA3]]|2.08|0.54|
|[[:human_genes:M:MDC1|MDC1]]|2.10|0.55|
|[[:human_genes:A:ATXN1|ATXN1]]|2.13|0.55|
|[[:human_genes:A:ATP13A1|ATP13A1]]|2.13|0.26|
|[[:human_genes:B:B4GALT2|B4GALT2]]|2.17|0.35|
|[[:human_genes:R:RBM42|RBM42]]|2.17|0.64|
|[[:human_genes:M:MTO1|MTO1]]|2.19|0.34|
|[[:human_genes:A:AAMP|AAMP]]|2.23|0.43|
|[[:human_genes:A:ABHD11|ABHD11]]|2.23|0.43|
|[[:human_genes:C:COX18|COX18]]|2.24|0.19|
|[[:human_genes:R:RRM2|RRM2]]|2.28|0.55|
|[[:human_genes:G:GUCY1B3|GUCY1B3]]|2.28|0.38|
|[[:human_genes:I:IMP4|IMP4]]|2.30|0.55|
|[[:human_genes:R:RUFY3|RUFY3]]|2.31|0.55|
|[[:human_genes:T:TSPY2|TSPY2]]|2.34|0.55|
|[[:human_genes:P:PDSS2|PDSS2]]|2.34|0.34|
|[[:human_genes:G:GMCL1|GMCL1]]|2.34|0.43|
|[[:human_genes:H:HAUS4|HAUS4]]|2.35|0.43|
|[[:human_genes:A:AJUBA|AJUBA]]|2.37|0.19|
|[[:human_genes:N:NUDT2|NUDT2]]|2.39|0.19|
|[[:human_genes:N:NDUFA13|NDUFA13]]|2.44|0.25|
|[[:human_genes:O:ORC6|ORC6]]|2.45|0.55|
|[[:human_genes:I:IRX3|IRX3]]|2.61|0.10|
|[[:human_genes:A:AWAT2|AWAT2]]|2.66|0.10|
|[[:human_genes:K:KIAA1984|KIAA1984]]|2.68|0.21|
|[[:human_genes:C:CCNA2|CCNA2]]|2.73|0.19|
|[[:human_genes:S:SFXN4|SFXN4]]|2.74|0.11|
|[[:human_genes:N:NDUFB11|NDUFB11]]|2.74|0.09|
|[[:human_genes:P:PET117|PET117]]|3.18|0.09|
|[[:human_genes:A:ADK|ADK]]|4.77|<0.01|
</modal>


<modal id="modal_screen_similarity" size="lg" title="Correlated Screens">
^Screen^Correlation^Plot^
|[[:results:exp160|Ribavirin 10 to 15μM on day4 R04 exp160]]|0.06|<button type="primary" size="small" modal="1">View</button>|
</modal>


<modal id="1" size="lg" title="Ribavirin 10 to 15μM on day4 R04 exp160  vs  Ribavirin 10μM R01 exp52">
{{:chemogenomics:comparison_plots:exp160_vs_exp52.png?nolink |}}
</modal>



<modal id="modal_go_sensitive" size="lg" title="Top 30 sensitive GO terms">
^GO Term^Fold Change^Genes^
|hypoxanthine biosynthetic process|570.86|PRPS1,HPRT1|
</modal>
<modal id="modal_go_resistant" size="lg" title="Top 30 resistant GO terms">
^GO Term^Fold Change^Genes^
|mitochondrial cytochrome c oxidase assembly|78.83|PET117,COX18,COA3|
|cytochrome complex assembly|48.69|PET117,COX18,COA3|
|mitochondrial respiratory chain complex assembly|31.71|PET117,NDUFB11,NDUFA13,COX18,COA3|
|purine nucleoside triphosphate biosynthetic process|26.92|ADK,NDUFB11,NDUFA13,NUDT2|
|nucleoside triphosphate biosynthetic process|23.73|ADK,NDUFB11,NDUFA13,NUDT2|
|purine ribonucleotide biosynthetic process|15.77|ADK,NDUFB11,NDUFA13,NUDT2,GUCY1B1|
|ribose phosphate biosynthetic process|14.01|ADK,NDUFB11,NDUFA13,NUDT2,GUCY1B1|
|nucleotide biosynthetic process|13.46|ADK,NDUFB11,NDUFA13,NUDT2,GUCY1B1,RRM2|
|nucleoside phosphate biosynthetic process|13.30|ADK,NDUFB11,NDUFA13,NUDT2,GUCY1B1,RRM2|
</modal>