CHD8 [The Human Chemical-Genetic Interaction Map Project]

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======= CHD8 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: CHD8
  * **<color #00a2e8>Official Name</color>**: chromodomain helicase DNA binding protein 8
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=57680|57680]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q9HCK8|Q9HCK8]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=CHD8&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20CHD8|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/610528|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  This gene encodes a member of the chromodomain-helicase-DNA binding protein family, which is characterized by a SNF2-like domain and two chromatin organization modifier domains. The encoded protein also contains brahma and kismet domains, which are common to the subfamily of chromodomain-helicase-DNA binding proteins to which this protein belongs. This gene has been shown to function in several processes that include transcriptional regulation, epigenetic remodeling, promotion of cell proliferation, and regulation of RNA synthesis. Allelic variants of this gene are associated with autism spectrum disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2016]. 
  * **<color #00a2e8>UniProt Summary</color>**:  DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1- targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription. {ECO:0000255|HAMAP- Rule:MF_03071, ECO:0000269|PubMed:17938208, ECO:0000269|PubMed:18378692}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|BRK|
|SNF2 N|
|Helicase C|
|Chromo|
|ResIII|
|DEAD|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|negative regulation of fibroblast apoptotic process|
|positive regulation of transcription by RNA polymerase III|
|armadillo repeat domain binding|
|prepulse inhibition|
|regulation of fibroblast apoptotic process|
|regulation of transcription by RNA polymerase III|
|startle response|
|MLL1 complex|
|DNA-dependent ATPase activity|
|social behavior|
|intraspecies interaction between organisms|
|DNA helicase activity|
|methylated histone binding|
|p53 binding|
|multi-organism behavior|
|ATP-dependent chromatin remodeling|
|beta-catenin binding|
|neuromuscular process|
|DNA duplex unwinding|
|DNA geometric change|
|histone binding|
|digestive tract development|
|digestive system development|
|chromatin remodeling|
|negative regulation of canonical Wnt signaling pathway|
|negative regulation of Wnt signaling pathway|
|regulation of canonical Wnt signaling pathway|
|DNA conformation change|
|cell-cell signaling by wnt|
|Wnt signaling pathway|
|regulation of Wnt signaling pathway|
|negative regulation of response to external stimulus|
|in utero embryonic development|
|chromatin binding|
|cell surface receptor signaling pathway involved in cell-cell signaling|
|behavior|
|protein-containing complex|
|chordate embryonic development|
|embryo development ending in birth or egg hatching|
|chromatin organization|
|brain development|
|head development|
|tube development|
|negative regulation of transcription by RNA polymerase II|
|negative regulation of apoptotic process|
|negative regulation of programmed cell death|
|embryo development|
|central nervous system development|
|negative regulation of cell death|
|chromosome organization|
|regulation of response to external stimulus|
|cell-cell signaling|
|negative regulation of transcription, DNA-templated|
|positive regulation of transcription by RNA polymerase II|
|negative regulation of nucleic acid-templated transcription|
|negative regulation of RNA biosynthetic process|
|negative regulation of signal transduction|
|negative regulation of RNA metabolic process|
|negative regulation of cell communication|
|negative regulation of signaling|
|nervous system process|
|negative regulation of cellular macromolecule biosynthetic process|
|negative regulation of nucleobase-containing compound metabolic process|
|DNA binding|
|negative regulation of macromolecule biosynthetic process|
|ATP binding|
|negative regulation of cellular biosynthetic process|
|positive regulation of transcription, DNA-templated|
|regulation of apoptotic process|
|negative regulation of biosynthetic process|
|regulation of programmed cell death|
|negative regulation of response to stimulus|
|positive regulation of nucleic acid-templated transcription|
|positive regulation of RNA biosynthetic process|
|regulation of cell death|
|negative regulation of gene expression|
|positive regulation of RNA metabolic process|
|positive regulation of nucleobase-containing compound metabolic process|
|positive regulation of macromolecule biosynthetic process|
|system process|
|positive regulation of cellular biosynthetic process|
|positive regulation of gene expression|
|positive regulation of biosynthetic process|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='default' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp525|Sulforaphane 9μM R08 exp525]]|-3.27|
|[[:results:exp454|Bafilomycin-A1 0.009μM R08 exp454]]|-2.6|
|[[:results:exp446|4-Nitroquinoline-1-oxide 0.5μM R08 exp446]]|-2.4|
|[[:results:exp500|LY2090314 0.003μM R08 exp500 no dilution day6]]|-2.4|
|[[:results:exp68|Clomiphene 4.4μM R02 exp68]]|-2.36|
|[[:results:exp316|Geldanamycin 0.015 to 0.025μM on day4 R07 exp316]]|-2.32|
|[[:results:exp517|Quercetin 20μM R08 exp517]]|-2.32|
|[[:results:exp243|S-trityl-L-cysteine 0.5μM R05 exp243]]|-2.3|
|[[:results:exp182|IU1-47 25μM R04 exp182]]|-2.26|
|[[:results:exp78|Pterostilbene 16μM R02 exp78]]|-2.26|
|[[:results:exp531|THZ1 0.06μM R08 exp531]]|-2.16|
|[[:results:exp485|GSK626616 14μM R08 exp485]]|-2.11|
|[[:results:exp274|Citral 50μM R06 exp274]]|-2.07|
|[[:results:exp467|CAY10603 0.55μM R08 exp467]]|-2.05|
|[[:results:exp294|Nutlin-3A 1.6μM R06 exp294]]|-2.05|
|[[:results:exp228|Demecolcine 0.03μM R05 exp228]]|-2.04|
|[[:results:exp122|Golgicide-A 4μM R03 exp122]]|-2.01|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|-1.98|
|[[:results:exp399|Salubrinal 20μM R07 exp399]]|-1.97|
|[[:results:exp490|Hydroxychloroquine 30μM R08 exp490]]|-1.96|
|[[:results:exp532|TIC10 10μM R08 exp532]]|-1.89|
|[[:results:exp357|Dorsomorphin 5μM R07 exp357]]|-1.86|
|[[:results:exp89|Vemurafenib 6.6μM R02 exp89]]|-1.85|
|[[:results:exp238|Parthenolide 2.5μM R05 exp238]]|-1.82|
|[[:results:exp434|Vemurafenib 6.6μM R08 exp434]]|-1.82|
|[[:results:exp447|Amiloride 100μM R08 exp447]]|-1.8|
|[[:results:exp502|Milciclib 2μM R08 exp502]]|-1.8|
|[[:results:exp436|Dynasore 7μM R08 exp436]]|-1.79|
|[[:results:exp311|2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311]]|-1.77|
|[[:results:exp180|Dynasore 10μM R04 exp180]]|-1.76|
|[[:results:exp400|Senexin-A 25μM R07 exp400]]|-1.76|
|[[:results:exp435|JQ1 0.8μM R08 exp435]]|-1.74|
|[[:results:exp135|MS023 7μM R03 exp135]]|-1.71|
|[[:results:exp79|Q15 2.7μM R02 exp79]]|-1.7|
|[[:results:exp306|Rapamycin 2μM R07 exp306]]|1.77|
|[[:results:exp303|39°C R06 exp303]]|1.88|
|[[:results:exp505|ML-792 0.2μM R08 exp505]]|2.29|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
No correlation found to any other genes in chemogenomics.
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/726
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/25|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/15|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/14|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/7|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 2292
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.76 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='CHD8 Expression in NALM6 Cells: 7.76'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>