CHD8

Gene Information

Official Symbol: CHD8
Official Name: chromodomain helicase DNA binding protein 8
Aliases and Previous Symbols: N/A
Entrez ID: 57680
UniProt: Q9HCK8
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: This gene encodes a member of the chromodomain-helicase-DNA binding protein family, which is characterized by a SNF2-like domain and two chromatin organization modifier domains. The encoded protein also contains brahma and kismet domains, which are common to the subfamily of chromodomain-helicase-DNA binding proteins to which this protein belongs. This gene has been shown to function in several processes that include transcriptional regulation, epigenetic remodeling, promotion of cell proliferation, and regulation of RNA synthesis. Allelic variants of this gene are associated with autism spectrum disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2016].
UniProt Summary: DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1- targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription. {ECO:0000255|HAMAP- Rule:MF_03071, ECO:0000269|PubMed:17938208, ECO:0000269|PubMed:18378692}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Sulforaphane 9μM R08 exp525	-3.27
Bafilomycin-A1 0.009μM R08 exp454	-2.6
4-Nitroquinoline-1-oxide 0.5μM R08 exp446	-2.4
LY2090314 0.003μM R08 exp500 no dilution day6	-2.4
Clomiphene 4.4μM R02 exp68	-2.36
Geldanamycin 0.015 to 0.025μM on day4 R07 exp316	-2.32
Quercetin 20μM R08 exp517	-2.32
S-trityl-L-cysteine 0.5μM R05 exp243	-2.3
IU1-47 25μM R04 exp182	-2.26
Pterostilbene 16μM R02 exp78	-2.26
THZ1 0.06μM R08 exp531	-2.16
GSK626616 14μM R08 exp485	-2.11
Citral 50μM R06 exp274	-2.07
CAY10603 0.55μM R08 exp467	-2.05
Nutlin-3A 1.6μM R06 exp294	-2.05
Demecolcine 0.03μM R05 exp228	-2.04
Golgicide-A 4μM R03 exp122	-2.01
BML-284 0.09μM R08 exp460	-1.98
Salubrinal 20μM R07 exp399	-1.97
Hydroxychloroquine 30μM R08 exp490	-1.96
TIC10 10μM R08 exp532	-1.89
Dorsomorphin 5μM R07 exp357	-1.86
Vemurafenib 6.6μM R02 exp89	-1.85
Parthenolide 2.5μM R05 exp238	-1.82
Vemurafenib 6.6μM R08 exp434	-1.82
Amiloride 100μM R08 exp447	-1.8
Milciclib 2μM R08 exp502	-1.8
Dynasore 7μM R08 exp436	-1.79
2-Methoxyestradiol 0.55 to 0.75μM on day4 R07 exp311	-1.77
Dynasore 10μM R04 exp180	-1.76
Senexin-A 25μM R07 exp400	-1.76
JQ1 0.8μM R08 exp435	-1.74
MS023 7μM R03 exp135	-1.71
Q15 2.7μM R02 exp79	-1.7
Rapamycin 2μM R07 exp306	1.77
39°C R06 exp303	1.88
ML-792 0.2μM R08 exp505	2.29

Global Fraction of Cell Lines Where Essential: 0/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/25
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/15
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/14
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/7
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 2292
Expression level (log2 read counts): 7.76

Expression Distribution

BRK
SNF2 N
Helicase C
Chromo
ResIII
DEAD

negative regulation of fibroblast apoptotic process
positive regulation of transcription by RNA polymerase III
armadillo repeat domain binding
prepulse inhibition
regulation of fibroblast apoptotic process
regulation of transcription by RNA polymerase III
startle response
MLL1 complex
DNA-dependent ATPase activity
social behavior
intraspecies interaction between organisms
DNA helicase activity
methylated histone binding
p53 binding
multi-organism behavior
ATP-dependent chromatin remodeling
beta-catenin binding
neuromuscular process
DNA duplex unwinding
DNA geometric change
histone binding
digestive tract development
digestive system development
chromatin remodeling
negative regulation of canonical Wnt signaling pathway
negative regulation of Wnt signaling pathway
regulation of canonical Wnt signaling pathway
DNA conformation change
cell-cell signaling by wnt
Wnt signaling pathway
regulation of Wnt signaling pathway
negative regulation of response to external stimulus
in utero embryonic development
chromatin binding
cell surface receptor signaling pathway involved in cell-cell signaling
behavior
protein-containing complex
chordate embryonic development
embryo development ending in birth or egg hatching
chromatin organization