DAPK3

Gene Information

Official Symbol: DAPK3
Official Name: death associated protein kinase 3
Aliases and Previous Symbols: N/A
Entrez ID: 1613
UniProt: O43293
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase- dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor. {ECO:0000269|PubMed:10356987, ECO:0000269|PubMed:11384979, ECO:0000269|PubMed:11781833, ECO:0000269|PubMed:12917339, ECO:0000269|PubMed:15096528, ECO:0000269|PubMed:15367680, ECO:0000269|PubMed:16219639, ECO:0000269|PubMed:17126281, ECO:0000269|PubMed:17158456, ECO:0000269|PubMed:18084323, ECO:0000269|PubMed:18995835, ECO:0000269|PubMed:21169990, ECO:0000269|PubMed:21408167, ECO:0000269|PubMed:21454679, ECO:0000269|PubMed:21487036, ECO:0000269|PubMed:23454120}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Ixabepilone 0.004 to 0.005μM on day4 R04 exp184	-1.8
Adefovir 20μM R07 exp332	1.7
All-trans-Retinoic-Acid 8μM R07 exp333	1.71
FBS-Wisent 0.1 R07 exp426	1.78

Global Fraction of Cell Lines Where Essential: 0/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/25
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/15
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/14
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/7
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 2462
Expression level (log2 read counts): 4.81

Expression Distribution

Pkinase
Pkinase Tyr

regulation of myosin II filament organization
cAMP response element binding protein binding
leucine zipper domain binding
positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
Rho GTPase binding
regulation of actin cytoskeleton reorganization
regulation of extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of extrinsic apoptotic signaling pathway
regulation of cell-substrate junction assembly
regulation of focal adhesion assembly
regulation of smooth muscle contraction
regulation of adherens junction organization
actin filament
regulation of cell junction assembly
PML body
regulation of cell-matrix adhesion
negative regulation of translation
positive regulation of canonical Wnt signaling pathway
negative regulation of cellular amide metabolic process
regulation of cell shape
regulation of extrinsic apoptotic signaling pathway
regulation of muscle contraction
cellular response to interferon-gamma
regulation of mitotic nuclear division
positive regulation of apoptotic signaling pathway
positive regulation of Wnt signaling pathway
response to interferon-gamma
protein C-terminus binding
regulation of nuclear division
protein autophosphorylation
regulation of cell-substrate adhesion
membrane raft
regulation of muscle system process
regulation of canonical Wnt signaling pathway
apoptotic signaling pathway
regulation of autophagy
regulation of actin cytoskeleton organization
regulation of translation
regulation of Wnt signaling pathway
protein serine/threonine kinase activity