NANOG

Gene Information

Official Symbol: N/ANOG
Official Name: Nanog homeobox
Aliases and Previous Symbols: N/A
Entrez ID: 79923
UniProt: Q9H9S0
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'- [CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. {ECO:0000269|PubMed:15983365, ECO:0000269|PubMed:16000880, ECO:0000269|PubMed:16391521, ECO:0000269|PubMed:25825768}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Global Fraction of Cell Lines Where Essential: 6/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/25
breast	1/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/15
kidney	0/21
liver	0/20
lung	2/75
lymphocyte	0/14
ovary	0/26
pancreas	0/24
peripheral nervous system	1/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/7
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 14025
Expression level (log2 read counts): 3.27

Expression Distribution

endodermal cell fate specification
endodermal cell fate commitment
cell fate commitment involved in formation of primary germ layer
positive regulation of stem cell proliferation
endodermal cell differentiation
endoderm formation
somatic stem cell population maintenance
regulation of stem cell proliferation
endoderm development
cell fate specification
formation of primary germ layer
stem cell population maintenance
maintenance of cell number
gastrulation
transcription regulatory region DNA binding
transcription corepressor activity
cell fate commitment
RNA polymerase II regulatory region sequence-specific DNA binding
sequence-specific DNA binding
DNA-binding transcription activator activity, RNA polymerase II-specific
RNA polymerase II proximal promoter sequence-specific DNA binding
embryonic morphogenesis
cytokine-mediated signaling pathway
DNA-binding transcription factor activity
nucleolus
anatomical structure formation involved in morphogenesis
positive regulation of cell population proliferation
embryo development
cellular response to cytokine stimulus
response to cytokine
positive regulation of transcription by RNA polymerase II
negative regulation of nucleic acid-templated transcription
negative regulation of RNA biosynthetic process
negative regulation of RNA metabolic process
negative regulation of nucleobase-containing compound metabolic process
DNA binding
negative regulation of macromolecule biosynthetic process
negative regulation of cellular biosynthetic process
positive regulation of transcription, DNA-templated
negative regulation of biosynthetic process