NCKAP1L [The Human Chemical-Genetic Interaction Map Project]

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======= NCKAP1L =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: NCKAP1L
  * **<color #00a2e8>Official Name</color>**: NCK associated protein 1 like
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=3071|3071]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/P55160|P55160]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=NCKAP1L&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20NCKAP1L|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/141180|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  Essential hematopoietic-specific regulator of the actin cytoskeleton (Probable). Controls lymphocyte development, activation, proliferation and homeostasis, erythrocyte membrane stability, as well as phagocytosis and migration by neutrophils and macrophages (PubMed:16417406, PubMed:17696648). Component of the WAVE2 complex which signals downstream of RAC to stimulate F- actin polymerization. Required for stabilization and/or translation of the WAVE2 complex proteins in hematopoietic cells (By similarity). Exhibits complex cycles of activation and inhibition to generate waves of propagating the assembly with actin (PubMed:16417406). Also involved in mechanisms WAVE- independent to regulate myosin and actin polymerization during neutrophil chemotaxis (PubMed:17696648). {ECO:0000250|UniProtKB:Q8K1X4, ECO:0000269|PubMed:16417406, ECO:0000269|PubMed:17696648, ECO:0000303|PubMed:20969869}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|Nckap1|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|positive regulation of gamma-delta T cell differentiation|
|positive regulation of CD8-positive, alpha-beta T cell differentiation|
|negative regulation of myosin-light-chain-phosphatase activity|
|regulation of gamma-delta T cell differentiation|
|positive regulation of gamma-delta T cell activation|
|positive regulation of CD8-positive, alpha-beta T cell activation|
|regulation of CD8-positive, alpha-beta T cell differentiation|
|actin polymerization-dependent cell motility|
|regulation of gamma-delta T cell activation|
|regulation of myosin-light-chain-phosphatase activity|
|SCAR complex|
|positive regulation of B cell differentiation|
|positive regulation of phagocytosis, engulfment|
|positive regulation of membrane invagination|
|negative regulation of interleukin-17 production|
|regulation of CD8-positive, alpha-beta T cell activation|
|regulation of phagocytosis, engulfment|
|regulation of membrane invagination|
|maintenance of cell polarity|
|positive regulation of cell adhesion mediated by integrin|
|positive regulation of neutrophil chemotaxis|
|B cell homeostasis|
|positive regulation of granulocyte chemotaxis|
|protein kinase activator activity|
|regulation of B cell differentiation|
|positive regulation of CD4-positive, alpha-beta T cell differentiation|
|regulation of neutrophil chemotaxis|
|positive regulation of neutrophil migration|
|regulation of interleukin-17 production|
|positive regulation of erythrocyte differentiation|
|erythrocyte development|
|T cell homeostasis|
|positive regulation of CD4-positive, alpha-beta T cell activation|
|regulation of neutrophil migration|
|positive regulation of B cell proliferation|
|cortical actin cytoskeleton organization|
|regulation of granulocyte chemotaxis|
|negative regulation of interleukin-6 production|
|regulation of cell adhesion mediated by integrin|
|regulation of erythrocyte differentiation|
|regulation of CD4-positive, alpha-beta T cell differentiation|
|positive regulation of alpha-beta T cell differentiation|
|cortical cytoskeleton organization|
|lymphocyte homeostasis|
|Rac GTPase binding|
|myeloid cell development|
|positive regulation of alpha-beta T cell activation|
|regulation of CD4-positive, alpha-beta T cell activation|
|negative regulation of phosphoprotein phosphatase activity|
|ficolin-1-rich granule membrane|
|regulation of B cell proliferation|
|regulation of alpha-beta T cell differentiation|
|negative regulation of protein dephosphorylation|
|vascular endothelial growth factor receptor signaling pathway|
|positive regulation of phagocytosis|
|leukocyte homeostasis|
|erythrocyte differentiation|
|neutrophil chemotaxis|
|positive regulation of T cell differentiation|
|positive regulation of leukocyte chemotaxis|
|granulocyte chemotaxis|
|erythrocyte homeostasis|
|positive regulation of actin filament polymerization|
|neutrophil migration|
|positive regulation of myeloid cell differentiation|
|regulation of alpha-beta T cell activation|
|positive regulation of lymphocyte differentiation|
|positive regulation of T cell proliferation|
|regulation of phagocytosis|
|secretory granule membrane|
|granulocyte migration|
|positive regulation of endocytosis|
|negative regulation of phosphatase activity|
|myeloid cell homeostasis|
|negative regulation of dephosphorylation|
|regulation of leukocyte chemotaxis|
|regulation of phosphoprotein phosphatase activity|
|positive regulation of protein polymerization|
|B cell receptor signaling pathway|
|myeloid leukocyte migration|
|positive regulation of leukocyte migration|
|positive regulation of lymphocyte proliferation|
|positive regulation of mononuclear cell proliferation|
|Fc-gamma receptor signaling pathway involved in phagocytosis|
|positive regulation of chemotaxis|
|immune response-regulating cell surface receptor signaling pathway involved in phagocytosis|
|positive regulation of leukocyte proliferation|
|regulation of T cell differentiation|
|Fc receptor mediated stimulatory signaling pathway|
|Fc-gamma receptor signaling pathway|
|regulation of protein dephosphorylation|
|regulation of interleukin-6 production|
|leukocyte chemotaxis|
|positive regulation of leukocyte differentiation|
|regulation of T cell proliferation|
|regulation of actin filament polymerization|
|positive regulation of B cell activation|
|regulation of lymphocyte differentiation|
|regulation of phosphatase activity|
|regulation of actin polymerization or depolymerization|
|regulation of actin filament length|
|positive regulation of hemopoiesis|
|regulation of leukocyte migration|
|establishment or maintenance of cell polarity|
|positive regulation of supramolecular fiber organization|
|homeostasis of number of cells|
|positive regulation of T cell activation|
|regulation of endocytosis|
|regulation of B cell activation|
|regulation of lymphocyte proliferation|
|regulation of mononuclear cell proliferation|
|cell chemotaxis|
|regulation of dephosphorylation|
|regulation of chemotaxis|
|positive regulation of leukocyte cell-cell adhesion|
|positive regulation of cytoskeleton organization|
|myeloid cell differentiation|
|regulation of leukocyte proliferation|
|regulation of protein polymerization|
|regulation of myeloid cell differentiation|
|Fc receptor signaling pathway|
|positive regulation of protein complex assembly|
|positive regulation of cell-cell adhesion|
|regulation of actin filament organization|
|protein-containing complex binding|
|regulation of leukocyte differentiation|
|negative regulation of cytokine production|
|GTPase activator activity|
|antigen receptor-mediated signaling pathway|
|regulation of leukocyte cell-cell adhesion|
|regulation of T cell activation|
|activation of protein kinase activity|
|phagocytosis|
|regulation of actin cytoskeleton organization|
|regulation of supramolecular fiber organization|
|positive regulation of lymphocyte activation|
|regulation of cellular component size|
|leukocyte migration|
|regulation of actin filament-based process|
|regulation of cell-cell adhesion|
|positive regulation of cell adhesion|
|positive regulation of leukocyte activation|
|positive regulation of GTPase activity|
|positive regulation of cell activation|
|immune response-activating cell surface receptor signaling pathway|
|regulation of protein complex assembly|
|regulation of hemopoiesis|
|negative regulation of hydrolase activity|
|cell projection assembly|
|neuron projection morphogenesis|
|immune response-regulating cell surface receptor signaling pathway|
|regulation of GTPase activity|
|plasma membrane bounded cell projection morphogenesis|
|neutrophil degranulation|
|cell projection morphogenesis|
|neutrophil activation involved in immune response|
|actin cytoskeleton organization|
|neutrophil mediated immunity|
|positive regulation of cell migration|
|neutrophil activation|
|granulocyte activation|
|transmembrane receptor protein tyrosine kinase signaling pathway|
|leukocyte degranulation|
|cell part morphogenesis|
|regulation of anatomical structure size|
|regulation of lymphocyte activation|
|myeloid leukocyte mediated immunity|
|positive regulation of cellular component biogenesis|
|myeloid cell activation involved in immune response|
|positive regulation of cell motility|
|positive regulation of protein kinase activity|
|positive regulation of cellular component movement|
|regulation of cytoskeleton organization|
|chemotaxis|
|regulation of vesicle-mediated transport|
|positive regulation of locomotion|
|taxis|
|hemopoiesis|
|negative regulation of phosphate metabolic process|
|negative regulation of phosphorus metabolic process|
|immune response-activating signal transduction|
|actin filament-based process|
|positive regulation of kinase activity|
|myeloid leukocyte activation|
|negative regulation of protein modification process|
|immune response-regulating signaling pathway|
|positive regulation of response to external stimulus|
|regulation of leukocyte activation|
|hematopoietic or lymphoid organ development|
|positive regulation of organelle organization|
|leukocyte activation involved in immune response|
|cell activation involved in immune response|
|activation of immune response|
|regulation of cell activation|
|immune system development|
|positive regulation of transferase activity|
|neuron projection development|
|regulation of cell adhesion|
|regulation of cytokine production|
|regulated exocytosis|
|enzyme linked receptor protein signaling pathway|
|cell morphogenesis|
|leukocyte mediated immunity|
|positive regulation of hydrolase activity|
|negative regulation of catalytic activity|
|exocytosis|
|regulation of protein kinase activity|
|neuron development|
|cellular component morphogenesis|
|regulation of cell migration|
|positive regulation of immune response|
|regulation of kinase activity|
|negative regulation of apoptotic process|
|negative regulation of programmed cell death|
|regulation of cell motility|
|positive regulation of cell population proliferation|
|leukocyte activation|
|regulation of cellular component biogenesis|
|cell migration|
|positive regulation of cell differentiation|
|regulation of transferase activity|
|regulation of locomotion|
|positive regulation of transport|
|negative regulation of cell death|
|regulation of cellular component movement|
|secretion by cell|
|neuron differentiation|
|positive regulation of protein phosphorylation|
|response to drug|
|negative regulation of cellular protein metabolic process|
|export from cell|
|positive regulation of phosphorylation|
|cell activation|
|cell motility|
|localization of cell|
|immune effector process|
|regulation of response to external stimulus|
|negative regulation of protein metabolic process|
|cytoskeleton organization|
|plasma membrane bounded cell projection organization|
|secretion|
|positive regulation of phosphorus metabolic process|
|positive regulation of phosphate metabolic process|
|negative regulation of molecular function|
|positive regulation of immune system process|
|regulation of immune response|
|cell projection organization|
|negative regulation of multicellular organismal process|
|positive regulation of cellular component organization|
|positive regulation of protein modification process|
|regulation of hydrolase activity|
|regulation of organelle organization|
|locomotion|
|positive regulation of developmental process|
|integral component of plasma membrane|
|positive regulation of catalytic activity|
|regulation of protein phosphorylation|
|generation of neurons|
|regulation of apoptotic process|
|movement of cell or subcellular component|
|protein-containing complex assembly|
|regulation of programmed cell death|
|regulation of phosphorylation|
|positive regulation of cellular protein metabolic process|
|regulation of cell population proliferation|
|neurogenesis|
|homeostatic process|
|cell development|
|regulation of immune system process|
|regulation of cell death|
|positive regulation of protein metabolic process|
|positive regulation of multicellular organismal process|
|positive regulation of molecular function|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|regulation of cell differentiation|
|regulation of protein modification process|
|protein-containing complex subunit organization|
|regulation of transport|
|immune response|
|vesicle-mediated transport|
|membrane|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp126|GSK461364A 0.1μM R03 exp126]]|-1.75|
|[[:results:exp210|LB-100 2μM R05 exp210]]|1.7|
|[[:results:exp308|Rapamycin 2μM plus FK-506 5μM R07 exp308]]|1.71|
|[[:results:exp54|Taxol 0.002μM R01 exp54]]|1.88|
|[[:results:exp184|Ixabepilone 0.004 to 0.005μM on day4 R04 exp184]]|1.89|
|[[:results:exp30|Rapamycin 10μM R00 exp30]]|2.16|
|[[:results:exp215|Colchicine 0.009μM R05 exp215]]|2.34|
|[[:results:exp45|Docetaxel 0.002μM R01 exp45]]|3.68|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:p:polr2j3|POLR2J3]]|0.504|
|[[:human genes:r:ruvbl2|RUVBL2]]|0.475|
|[[:human genes:p:ppp3ca|PPP3CA]]|0.464|
|[[:human genes:c:cdh24|CDH24]]|0.452|
|[[:human genes:c:clasp1|CLASP1]]|0.448|
|[[:human genes:m:med14|MED14]]|0.446|
|[[:human genes:a:arpc4|ARPC4]]|0.443|
|[[:human genes:o:osbp|OSBP]]|0.441|
|[[:human genes:o:orc6|ORC6]]|0.441|
|[[:human genes:a:actr2|ACTR2]]|0.44|
|[[:human genes:i:itpr1|ITPR1]]|0.438|
|[[:human genes:c:copb1|COPB1]]|0.438|
|[[:human genes:s:slc4a1|SLC4A1]]|0.417|
|[[:human genes:s:supt6h|SUPT6H]]|0.416|
|[[:human genes:o:orc1|ORC1]]|0.415|
|[[:human genes:r:rad51|RAD51]]|0.413|
|[[:human genes:t:ticrr|TICRR]]|0.41|
|[[:human genes:n:napa|NAPA]]|0.41|
|[[:human genes:n:numa1|NUMA1]]|0.406|
|[[:human genes:n:nup160|NUP160]]|0.406|
|[[:human genes:m:mbtd1|MBTD1]]|0.404|
|[[:human genes:t:tfip11|TFIP11]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 2/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|1/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 1693
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 7.7 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='NCKAP1L Expression in NALM6 Cells: 7.7'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>