NPLOC4

Gene Information

Official Symbol: NPLOC4
Official Name: NPL4 homolog, ubiquitin recognition factor
Aliases and Previous Symbols: N/A
Entrez ID: 55666
UniProt: Q8TAT6
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and UFD1, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I (PubMed:26471729). {ECO:0000250|UniProtKB:Q9ES54, ECO:0000269|PubMed:26471729}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
CB-5083 0.4μM R08 exp468	-2.73
THZ531 0.11 to 0.125 to 0.35μM on day4 then day6 R07 exp412	1.7
Fluvastatin 2.2μM R06 exp282	1.72
Bortezomib 0.05μM R00 exp7	1.76
THZ531 0.11μM R07 exp409	1.94
THZ531 0.11 to 0.175μM on day4 R07 exp413	2.06
THZ531 0.11 to 0.125μM on day4 R07 exp410	2.46

Global Fraction of Cell Lines Where Essential: 599/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	0/1
bile duct	19/28
blood	27/28
bone	24/26
breast	29/33
central nervous system	48/56
cervix	4/4
colorectal	15/17
esophagus	8/13
fibroblast	1/1
gastric	13/16
kidney	17/21
liver	16/20
lung	57/75
lymphocyte	13/16
ovary	21/26
pancreas	18/24
peripheral nervous system	9/16
plasma cell	14/15
prostate	1/1
skin	18/24
soft tissue	9/9
thyroid	2/2
upper aerodigestive	13/22
urinary tract	22/29
uterus	4/5

Essentiality in NALM6

Essentiality Rank: 936
Expression level (log2 read counts): 6.93

Expression Distribution

UN NPL4
zf-NPL4
NPL4

UFD1-NPL4 complex
nuclear outer membrane-endoplasmic reticulum membrane network
VCP-NPL4-UFD1 AAA ATPase complex
negative regulation of RIG-I signaling pathway
negative regulation of viral-induced cytoplasmic pattern recognition receptor signaling pathway
retrograde protein transport, ER to cytosol
regulation of RIG-I signaling pathway
endoplasmic reticulum to cytosol transport
error-free translesion synthesis
regulation of viral-induced cytoplasmic pattern recognition receptor signaling pathway
negative regulation of defense response to virus
protein exit from endoplasmic reticulum
translesion synthesis
negative regulation of type I interferon production
DNA synthesis involved in DNA repair
postreplication repair
ubiquitin-dependent ERAD pathway
regulation of defense response to virus
ubiquitin binding
ERAD pathway
negative regulation of response to biotic stimulus
DNA biosynthetic process
negative regulation of immune effector process
regulation of type I interferon production
Golgi organization
negative regulation of defense response
negative regulation of multi-organism process
response to endoplasmic reticulum stress
negative regulation of cytokine production
ubiquitin protein ligase binding
proteasome-mediated ubiquitin-dependent protein catabolic process
proteasomal protein catabolic process
negative regulation of response to external stimulus
endomembrane system organization
negative regulation of immune system process
regulation of innate immune response
regulation of immune effector process
negative regulation of intracellular signal transduction
DNA repair
regulation of response to biotic stimulus