NPRL2 [The Human Chemical-Genetic Interaction Map Project]

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======= NPRL2 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: NPRL2
  * **<color #00a2e8>Official Name</color>**: NPR2 like, GATOR1 complex subunit
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=10641|10641]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q8WTW4|Q8WTW4]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=NPRL2&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20NPRL2|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/607072|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**: N/A
  * **<color #00a2e8>UniProt Summary</color>**:  As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway. {ECO:0000269|PubMed:23723238}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|NPR2|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|GATOR1 complex|
|cellular response to nitrogen levels|
|cellular response to nitrogen starvation|
|regulation of autophagosome assembly|
|regulation of vacuole organization|
|cellular response to amino acid starvation|
|response to amino acid starvation|
|negative regulation of TOR signaling|
|regulation of TOR signaling|
|positive regulation of autophagy|
|cellular response to starvation|
|regulation of macroautophagy|
|response to starvation|
|regulation of organelle assembly|
|cellular response to nutrient levels|
|protein kinase activity|
|negative regulation of kinase activity|
|cellular response to extracellular stimulus|
|negative regulation of transferase activity|
|GTPase activator activity|
|lysosomal membrane|
|regulation of autophagy|
|cellular response to external stimulus|
|positive regulation of cellular catabolic process|
|positive regulation of GTPase activity|
|positive regulation of catabolic process|
|negative regulation of phosphorylation|
|regulation of GTPase activity|
|response to nutrient levels|
|negative regulation of intracellular signal transduction|
|response to extracellular stimulus|
|negative regulation of phosphate metabolic process|
|negative regulation of phosphorus metabolic process|
|positive regulation of hydrolase activity|
|negative regulation of catalytic activity|
|regulation of cellular catabolic process|
|regulation of kinase activity|
|regulation of cellular component biogenesis|
|protein phosphorylation|
|regulation of transferase activity|
|regulation of catabolic process|
|negative regulation of molecular function|
|negative regulation of signal transduction|
|regulation of hydrolase activity|
|phosphorylation|
|regulation of organelle organization|
|negative regulation of cell communication|
|negative regulation of signaling|
|positive regulation of catalytic activity|
|regulation of phosphorylation|
|negative regulation of response to stimulus|
|cellular response to stress|
|positive regulation of molecular function|
|regulation of phosphate metabolic process|
|regulation of phosphorus metabolic process|
|regulation of intracellular signal transduction|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp500|LY2090314 0.003μM R08 exp500 no dilution day6]]|-4.14|
|[[:results:exp175|3-Bromopyruvate 7μM R04 exp175]]|-3.99|
|[[:results:exp227|Cryptotanshinone 12μM R05 exp227]]|-3.8|
|[[:results:exp356|Docosahexaenoic-acid 50μM R07 exp356]]|-3.63|
|[[:results:exp499|LY2090314 0.003μM R08 exp499]]|-3.53|
|[[:results:exp190|Vincristine 0.0005μM R04 exp190]]|-3.37|
|[[:results:exp468|CB-5083 0.4μM R08 exp468]]|-3.13|
|[[:results:exp450|Artemisinin 50μM R08 exp450]]|-3.12|
|[[:results:exp184|Ixabepilone 0.004 to 0.005μM on day4 R04 exp184]]|-2.99|
|[[:results:exp416|Tubacin 1.6μM R07 exp416]]|-2.97|
|[[:results:exp329|Hydroxyurea 100μM R07 exp329]]|-2.95|
|[[:results:exp525|Sulforaphane 9μM R08 exp525]]|-2.92|
|[[:results:exp228|Demecolcine 0.03μM R05 exp228]]|-2.91|
|[[:results:exp36|TRAIL 50ng/ml R00 exp36]]|-2.83|
|[[:results:exp526|Sulforaphane 9μM R08 exp526 no dilution day6]]|-2.81|
|[[:results:exp360|Genistein 15μM R07 exp360]]|-2.76|
|[[:results:exp517|Quercetin 20μM R08 exp517]]|-2.74|
|[[:results:exp354|Diepoxybutane 3μM R07 exp354]]|-2.73|
|[[:results:exp290|LLY-283 2.6μM R06 exp290]]|-2.72|
|[[:results:exp515|PU-H71 1μM R08 exp515]]|-2.72|
|[[:results:exp211|AICAR 240μM R05 exp211]]|-2.64|
|[[:results:exp294|Nutlin-3A 1.6μM R06 exp294]]|-2.61|
|[[:results:exp187|proTAME 5μM R04 exp187]]|-2.6|
|[[:results:exp84|UM0125461 0.74μM R02 exp84]]|-2.53|
|[[:results:exp335|Aminopterin 0.005μM R07 exp335]]|-2.49|
|[[:results:exp311|2-Methoxyestradiol 0.55 to 0.75μM  on day4 R07 exp311]]|-2.47|
|[[:results:exp312|2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312]]|-2.45|
|[[:results:exp198|Etoposide 0.1μM R05 exp198]]|-2.45|
|[[:results:exp445|∆-9-Tetrahydrocannabinol 30μM R08 exp445]]|-2.42|
|[[:results:exp125|GSK461364A 0.005μM R03 exp125]]|-2.37|
|[[:results:exp6|Bortezomib 0.005μM R00 exp6]]|-2.35|
|[[:results:exp78|Pterostilbene 16μM R02 exp78]]|-2.35|
|[[:results:exp215|Colchicine 0.009μM R05 exp215]]|-2.29|
|[[:results:exp122|Golgicide-A 4μM R03 exp122]]|-2.28|
|[[:results:exp247|UM0130462 0.025 to 0.035μM day4 R05 exp247]]|-2.25|
|[[:results:exp239|PFI-2 4μM R05 exp239]]|-2.21|
|[[:results:exp176|Apcin 50 to 100μM on day4 R04 exp176]]|-2.2|
|[[:results:exp180|Dynasore 10μM R04 exp180]]|-2.2|
|[[:results:exp243|S-trityl-L-cysteine 0.5μM R05 exp243]]|-2.16|
|[[:results:exp238|Parthenolide 2.5μM R05 exp238]]|-2.14|
|[[:results:exp162|BI-D1870 2μM R04 exp162]]|-2.13|
|[[:results:exp350|Deferoxamine 11μM R07 exp350]]|-2.13|
|[[:results:exp520|Rucaparib 6.5μM R08 exp520]]|-2.13|
|[[:results:exp529|Thimerosal 0.85μM R08 exp529]]|-2.13|
|[[:results:exp35|TRAIL 5ng/ml R00 exp35]]|-2.12|
|[[:results:exp66|BI-D1870 3.15μM R02 exp66]]|-2.1|
|[[:results:exp505|ML-792 0.2μM R08 exp505]]|-2.1|
|[[:results:exp165|RO-3306 3 to 4μM on day4 R04 exp165]]|-2.07|
|[[:results:exp288|HMS-I2 10μM R06 exp288]]|-2.06|
|[[:results:exp430|Rifampicin 30μM R08 exp430]]|-2.06|
|[[:results:exp230|Epigallocatechin gallate 20μM R05 exp230]]|-2.03|
|[[:results:exp128|GSK591 2.6μM R03 exp128]]|-2.03|
|[[:results:exp177|Apcin 25μM plus proTAME 2μM R04 exp177]]|-2.02|
|[[:results:exp159|Docetaxel 0.001 to 0.002μM on day4 R04 exp159]]|-2.02|
|[[:results:exp231|Epothilone-B 0.0015μM R05 exp231]]|-2|
|[[:results:exp179|Combretastatin A4 0.002 to 0.003μM day4 R04 exp179]]|-1.96|
|[[:results:exp274|Citral 50μM R06 exp274]]|-1.95|
|[[:results:exp379|MSC2530818 10μM R07 exp379]]|-1.95|
|[[:results:exp245|UM0011500 5μM R05 exp245]]|-1.94|
|[[:results:exp60|Vinblastine 0.002μM R01 exp60]]|-1.92|
|[[:results:exp223|Cabazitaxel 0.001μM R05 exp223]]|-1.91|
|[[:results:exp358|FK-506 5μM R07 exp358]]|-1.9|
|[[:results:exp291|LLY-284 2.6μM R06 exp291]]|-1.89|
|[[:results:exp340|BN82002 4μM R07 exp340]]|-1.88|
|[[:results:exp460|BML-284 0.09μM R08 exp460]]|-1.87|
|[[:results:exp246|UM0011500 10μM R05 exp246]]|-1.87|
|[[:results:exp365|I-BRD9 4μM R07 exp365]]|-1.85|
|[[:results:exp443|SNS-032 15μM R08 exp443]]|-1.85|
|[[:results:exp436|Dynasore 7μM R08 exp436]]|-1.84|
|[[:results:exp466|Cannabidiol 20μM R08 exp466]]|-1.82|
|[[:results:exp107|UMK57 0.6μM R03 exp107]]|-1.82|
|[[:results:exp461|BS-181 20μM R08 exp461]]|-1.8|
|[[:results:exp446|4-Nitroquinoline-1-oxide 0.5μM R08 exp446]]|-1.79|
|[[:results:exp183|IU1-C 25μM R04 exp183]]|-1.78|
|[[:results:exp390|Pifithrin-alpha 20μM R07 exp390]]|-1.76|
|[[:results:exp45|Docetaxel 0.002μM R01 exp45]]|-1.75|
|[[:results:exp533|TNF-alpha 44ng/ml R08 exp533]]|-1.75|
|[[:results:exp242|Radicicol 0.16μM R05 exp242]]|-1.74|
|[[:results:exp474|CR131-b 0.005μM R08 exp474]]|-1.72|
|[[:results:exp67|BVD-523 15μM R02 exp67]]|-1.71|
|[[:results:exp513|ONC212 0.15μM R08 exp513]]|-1.7|
|[[:results:exp47|Lapatinib 5μM R01 exp47]]|1.73|
|[[:results:exp269|Bisphenol A 100μM R06 exp269]]|1.75|
|[[:results:exp434|Vemurafenib 6.6μM R08 exp434]]|1.77|
|[[:results:exp151|SGC0946 7μM R03 exp151]]|1.96|
|[[:results:exp489|Hippuristanol 0.12μM R08 exp489 no dilution day6]]|1.99|
|[[:results:exp530|Thioridazine 5μM R08 exp530]]|1.99|
|[[:results:exp490|Hydroxychloroquine 30μM R08 exp490]]|2.06|
|[[:results:exp303|39°C R06 exp303]]|2.11|
|[[:results:exp382|Palbociclib 1μM R07 exp382]]|2.15|
|[[:results:exp233|EPZ-5676 30μM R05 exp233]]|2.4|
|[[:results:exp514|Phorbol-12-myristate-13-acetate 0.57μM R08 exp514]]|2.41|
|[[:results:exp488|Hippuristanol 0.12μM R08 exp488]]|2.48|
|[[:results:exp25|Oligomycin-A 2μM R00 exp25]]|2.57|
|[[:results:exp189|Temozolomide 200μM R04 exp189]]|2.58|
|[[:results:exp453|B02 10μM R08 exp453]]|2.61|
|[[:results:exp506|Momordin-Ic 10μM R08 exp506]]|2.67|
|[[:results:exp216|Erlotinib 10μM R05 exp216]]|3.13|
|[[:results:exp510|Nicotine 3000μM R08 exp510]]|3.18|
|[[:results:exp389|PF-06409577 20μM R07 exp389]]|3.27|
|[[:results:exp352|Dexamethasone 0.006 to 0.015μM on day4 R07 exp352]]|3.87|
|[[:results:exp485|GSK626616 14μM R08 exp485]]|4.82|
|[[:results:exp504|MK2206 4μM R08 exp504]]|7.7|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:n:nprl3|NPRL3]]|0.849|
|[[:human genes:d:depdc5|DEPDC5]]|0.808|
|[[:human genes:s:szt2|SZT2]]|0.796|
|[[:human genes:k:kptn|KPTN]]|0.737|
|[[:human genes:i:itfg2|ITFG2]]|0.713|
|[[:human genes:c:c12orf66|C12orf66]]|0.691|
|[[:human genes:t:tsc2|TSC2]]|0.664|
|[[:human genes:t:tsc1|TSC1]]|0.626|
|[[:human genes:s:sesn2|SESN2]]|0.591|
|[[:human genes:c:ccdc130|CCDC130]]|0.485|
|[[:human genes:z:zfp36l2|ZFP36L2]]|0.464|
|[[:human genes:m:msi2|MSI2]]|0.43|
|[[:human genes:f:fam122a|FAM122A]]|0.414|
|[[:human genes:a:anp32a|ANP32A]]|0.411|
|[[:human genes:n:nans|NANS]]|0.402|
|[[:human genes:i:ippk|IPPK]]|0.401|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 0/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|0/1|
|909776.0|0/1|
|bile duct|0/28|
|blood|0/28|
|bone|0/26|
|breast|0/33|
|central nervous system|0/56|
|cervix|0/4|
|colorectal|0/17|
|esophagus|0/13|
|fibroblast|0/1|
|gastric|0/16|
|kidney|0/21|
|liver|0/20|
|lung|0/75|
|lymphocyte|0/16|
|ovary|0/26|
|pancreas|0/24|
|peripheral nervous system|0/16|
|plasma cell|0/15|
|prostate|0/1|
|skin|0/24|
|soft tissue|0/9|
|thyroid|0/2|
|upper aerodigestive|0/22|
|urinary tract|0/29|
|uterus|0/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 12763
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 4.59 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='NPRL2 Expression in NALM6 Cells: 4.59'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>