NUDT16

Gene Information

Official Symbol: NUDT16
Official Name: nudix hydrolase 16
Aliases and Previous Symbols: N/A
Entrez ID: 131870
UniProt: Q96DE0
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal- dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Catalyzes also the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG). Also hydrolysis m7G- and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA; metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Acts also as a phosphatase; hydrolyzes the non-canonical purine nucleotides inosine diphosphate (IDP) and deoxyinosine diphosphate (dITP) as well as guanosine diphosphate (GDP), deoxyguanosine diphosphate (dGDP), xanthine diphosphate (XDP), inosine triphosphate (ITP) and deoxyinosine triphosphate (ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy- and ribose forms (PubMed:20385596, PubMed:26121039). The order of activity with different substrates is IDP > dIDP » GDP = dGDP > XDP = ITP = dITP (PubMed:20385596). Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions (PubMed:20385596). {ECO:0000269|PubMed:15053875, ECO:0000269|PubMed:17567574, ECO:0000269|PubMed:18820299, ECO:0000269|PubMed:20385596, ECO:0000269|PubMed:21070968, ECO:0000269|PubMed:21337011, ECO:0000269|PubMed:26121039}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Global Fraction of Cell Lines Where Essential: 0/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 8989
Expression level (log2 read counts): 3.49

Expression Distribution

XTP binding
XDP catabolic process
dITP metabolic process
ITP binding
dITP catabolic process
IDP metabolic process
dITP diphosphatase activity
negative regulation of rRNA processing
IDP catabolic process
snoRNA catabolic process
cobalt ion binding
phosphodiesterase decapping endonuclease activity
purine ribonucleoside diphosphate catabolic process
negative regulation of ribosome biogenesis
purine nucleoside diphosphate catabolic process
nucleotide phosphatase activity, acting on free nucleotides
purine deoxyribonucleoside triphosphate catabolic process
purine deoxyribonucleotide catabolic process
purine nucleoside triphosphate catabolic process
ribonucleoside diphosphate catabolic process
nucleoside diphosphate catabolic process
purine deoxyribonucleoside triphosphate metabolic process
deoxyribonucleoside triphosphate catabolic process
adenosine to inosine editing
m7G(5)pppN diphosphatase activity
purine deoxyribonucleotide metabolic process
metalloexopeptidase activity
regulation of rRNA processing
nucleoside triphosphate catabolic process
chloride ion binding
regulation of ribosome biogenesis
snoRNA binding
snoRNA metabolic process
deoxyribonucleoside triphosphate metabolic process
deoxyribonucleotide catabolic process
deoxyribose phosphate catabolic process
base conversion or substitution editing
ncRNA catabolic process
positive regulation of double-strand break repair
2-deoxyribonucleotide metabolic process