PRPF8 [The Human Chemical-Genetic Interaction Map Project]

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======= PRPF8 =======


== Gene Information ==
  * **<color #00a2e8>Official Symbol</color>**: PRPF8
  * **<color #00a2e8>Official Name</color>**: pre-mRNA processing factor 8
  * **<color #00a2e8>Aliases and Previous Symbols</color>**: N/A
  * **<color #00a2e8>Entrez ID</color>**: [[https://www.ncbi.nlm.nih.gov/gene/?term=10594|10594]]
  * **<color #00a2e8>UniProt</color>**: [[https://www.uniprot.org/uniprot/Q6P2Q9|Q6P2Q9]]
  * **<color #00a2e8>Interactions</color>**: [[https://thebiogrid.org/search.php?search=PRPF8&organism=9606|BioGRID]]
  * **<color #00a2e8>PubMed articles</color>**: [[https://www.ncbi.nlm.nih.gov/pubmed/?term=gene%20PRPF8|Open PubMed]]
  * **<color #00a2e8>OMIM</color>**: [[https://omim.org/entry/607300|Open OMIM]]

== Function Summary ==
  * **<color #00a2e8>Entrez Summary</color>**:  Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq, Jul 2008]. 
  * **<color #00a2e8>UniProt Summary</color>**:  Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site. {ECO:0000269|PubMed:20595234, ECO:0000303|PubMed:15840809}.

<button type='primary' size='sm' modal='Pfam_Domains'>Pfam Domains</button>
<button type='primary' size='sm' modal='GO_terms'>GO Terms</button>


<modal id='Pfam_Domains' size='lg' title='Pfam Domains'>
|U6-snRNA bdg|
|U5 2-snRNA bdg|
|PRP8 domainIV|
|PRO8NT|
|PROCT|
|RRM 4|
|PROCN|
|Mov34|
</modal>

<modal id='GO_terms' size='lg' title='GO Terms'>
|U5 snRNA binding|
|second spliceosomal transesterification activity|
|U2 snRNA binding|
|U1 snRNA binding|
|pre-mRNA intronic binding|
|U6 snRNA binding|
|U2-type catalytic step 1 spliceosome|
|spliceosomal tri-snRNP complex assembly|
|U5 snRNP|
|K63-linked polyubiquitin modification-dependent protein binding|
|U2-type catalytic step 2 spliceosome|
|U4/U6 x U5 tri-snRNP complex|
|spliceosomal snRNP assembly|
|U2-type precatalytic spliceosome|
|catalytic step 2 spliceosome|
|cellular response to lipopolysaccharide|
|cellular response to molecule of bacterial origin|
|cellular response to biotic stimulus|
|ribonucleoprotein complex assembly|
|cellular response to tumor necrosis factor|
|ribonucleoprotein complex subunit organization|
|response to tumor necrosis factor|
|RNA splicing, via transesterification reactions with bulged adenosine as nucleophile|
|mRNA splicing, via spliceosome|
|RNA splicing, via transesterification reactions|
|response to lipopolysaccharide|
|response to molecule of bacterial origin|
|RNA splicing|
|nuclear speck|
|ribonucleoprotein complex biogenesis|
|mRNA processing|
|cellular response to lipid|
|response to bacterium|
|mRNA metabolic process|
|cellular protein-containing complex assembly|
|response to lipid|
|RNA processing|
|cellular response to cytokine stimulus|
|cellular response to oxygen-containing compound|
|response to cytokine|
|response to other organism|
|response to external biotic stimulus|
|response to biotic stimulus|
|RNA binding|
|response to oxygen-containing compound|
|protein-containing complex assembly|
|RNA metabolic process|
|protein-containing complex subunit organization|
|membrane|
|gene expression|
</modal>
\\

 === CRISPR Data ===
<button type='primary' size='small' modal='Compound_Hit'>Compound Hit</button>
<button type='primary' size='small' modal='Most_Correlated_Genes'>Most Correlated Genes in Chemogenomics</button>
<button type='primary' size='small' modal='Essential_Avana'>Tissues where Essential in the Avana Dataset (DepMap 20Q1)</button>

<modal id='Compound_Hit' size='lg' title='Compound Hit'>
^Screen^Score^
|[[:results:exp7|Bortezomib 0.05μM R00 exp7]]|-2.21|
|[[:results:exp487|Hinokiflavone 12μM R08 exp487]]|-2.14|
|[[:results:exp536|Vitamin-D3 40μM R08 exp536]]|-2.07|
|[[:results:exp485|GSK626616 14μM R08 exp485]]|-2.03|
|[[:results:exp431|Rotenone 0.07μM R08 exp431]]|-1.76|
|[[:results:exp525|Sulforaphane 9μM R08 exp525]]|-1.76|
|[[:results:exp505|ML-792 0.2μM R08 exp505]]|-1.74|
|[[:results:exp448|Ammonium tetrathiomolybdate 10μM R08 exp448]]|-1.71|
|[[:results:exp506|Momordin-Ic 10μM R08 exp506]]|1.71|
|[[:results:exp461|BS-181 20μM R08 exp461]]|1.76|
|[[:results:exp160|Ribavirin 10 to 15μM on day4 R04 exp160]]|1.78|
|[[:results:exp450|Artemisinin 50μM R08 exp450]]|1.84|
|[[:results:exp538|ZLN024 50μM R08 exp538]]|1.86|
|[[:results:exp14|Cycloheximide 0.02μM R00 exp14]]|1.87|
|[[:results:exp501|Methotrexate 0.01μM R08 exp501]]|1.93|
|[[:results:exp184|Ixabepilone 0.004 to 0.005μM on day4 R04 exp184]]|2.03|
|[[:results:exp10|CCCP 0.1μM R00 exp10]]|2.38|
|[[:results:exp23|Nocodazole 0.02μM R00 exp23]]|2.4|
|[[:results:exp144|PFI-3 10μM R03 exp144]]|2.56|
|[[:results:exp12|Chloramphenicol 2μM R00 exp12]]|2.59|
|[[:results:exp81|Selumetinib 20μM R02 exp81]]|2.81|
|[[:results:exp5|Alpha-Amanitin 0.5μM R00 exp5]]|3|
</modal>

<modal id='Most_Correlated_Genes' size='lg' title='Most Correlated Genes in Chemogenomics'>
^Gene^Correlation^
|[[:human genes:v:vasn|VASN]]|0.506|
|[[:human genes:r:rps19bp1|RPS19BP1]]|0.497|
|[[:human genes:d:dtl|DTL]]|0.49|
|[[:human genes:t:tbc1d3h|TBC1D3H]]|0.483|
|[[:human genes:s:scaf1|SCAF1]]|0.473|
|[[:human genes:t:tbc1d3c|TBC1D3C]]|0.472|
|[[:human genes:t:tbc1d3k|TBC1D3K]]|0.465|
|[[:human genes:t:tbc1d3i|TBC1D3I]]|0.464|
|[[:human genes:t:tbc1d3l|TBC1D3L]]|0.464|
|[[:human genes:t:tbc1d3e|TBC1D3E]]|0.464|
|[[:human genes:m:myo1h|MYO1H]]|0.46|
|[[:human genes:p:psmb1|PSMB1]]|0.457|
|[[:human genes:c:chaf1a|CHAF1A]]|0.457|
|[[:human genes:r:rnf4|RNF4]]|0.456|
|[[:human genes:s:sec13|SEC13]]|0.452|
|[[:human genes:e:esr2|ESR2]]|0.451|
|[[:human genes:d:dido1|DIDO1]]|0.451|
|[[:human genes:n:nup85|NUP85]]|0.45|
|[[:human genes:s:sprr2b|SPRR2B]]|0.444|
|[[:human genes:r:rps2|RPS2]]|0.444|
|[[:human genes:p:polr2j3|POLR2J3]]|0.443|
|[[:human genes:t:tbc1d3|TBC1D3]]|0.443|
|[[:human genes:e:elmod3|ELMOD3]]|0.442|
|[[:human genes:t:tpx2|TPX2]]|0.442|
|[[:human genes:t:tbc1d3g|TBC1D3G]]|0.44|
|[[:human genes:r:rad51|RAD51]]|0.438|
|[[:human genes:p:psmd14|PSMD14]]|0.437|
|[[:human genes:p:pola2|POLA2]]|0.435|
|[[:human genes:p:psma6|PSMA6]]|0.435|
|[[:human genes:c:cdk9|CDK9]]|0.434|
|[[:human genes:z:zfand5|ZFAND5]]|0.434|
|[[:human genes:z:znf655|ZNF655]]|0.433|
|[[:human genes:k:krt78|KRT78]]|0.433|
|[[:human genes:p:psmd1|PSMD1]]|0.432|
|[[:human genes:l:lrr1|LRR1]]|0.431|
|[[:human genes:r:rfc1|RFC1]]|0.431|
|[[:human genes:t:tbc1d3f|TBC1D3F]]|0.429|
|[[:human genes:m:myt1|MYT1]]|0.426|
|[[:human genes:g:golga6b|GOLGA6B]]|0.425|
|[[:human genes:r:rps12|RPS12]]|0.425|
|[[:human genes:d:dnttip2|DNTTIP2]]|0.424|
|[[:human genes:s:sh3bp1|SH3BP1]]|0.423|
|[[:human genes:b:brd4|BRD4]]|0.423|
|[[:human genes:c:cwc22|CWC22]]|0.42|
|[[:human genes:c:cmya5|CMYA5]]|0.42|
|[[:human genes:g:gjc3|GJC3]]|0.419|
|[[:human genes:h:hap1|HAP1]]|0.419|
|[[:human genes:s:snrnp200|SNRNP200]]|0.418|
|[[:human genes:r:rpl35|RPL35]]|0.417|
|[[:human genes:m:mbd3l2|MBD3L2]]|0.415|
|[[:human genes:n:nup188|NUP188]]|0.414|
|[[:human genes:n:nup107|NUP107]]|0.414|
|[[:human genes:t:twistnb|TWISTNB]]|0.414|
|[[:human genes:p:psmb2|PSMB2]]|0.414|
|[[:human genes:r:rrp1|RRP1]]|0.413|
|[[:human genes:s:smc3|SMC3]]|0.412|
|[[:human genes:n:nup160|NUP160]]|0.411|
|[[:human genes:s:sart1|SART1]]|0.411|
|[[:human genes:s:sf3a1|SF3A1]]|0.411|
|[[:human genes:i:ints6|INTS6]]|0.41|
|[[:human genes:p:plrg1|PLRG1]]|0.409|
|[[:human genes:o:or10g3|OR10G3]]|0.409|
|[[:human genes:r:rps28|RPS28]]|0.407|
|[[:human genes:z:znf106|ZNF106]]|0.407|
|[[:human genes:c:c1qtnf9b|C1QTNF9B]]|0.406|
|[[:human genes:h:hcfc1|HCFC1]]|0.404|
|[[:human genes:s:slc38a7|SLC38A7]]|0.404|
|[[:human genes:s:sf3a3|SF3A3]]|0.401|
|[[:human genes:s:sprr2a|SPRR2A]]|0.401|
|[[:human genes:c:c14orf93|C14orf93]]|0.401|
|[[:human genes:s:sema6b|SEMA6B]]|0.4|
</modal>

<modal id='Essential_Avana' size='lg' title='Tissues where Essential in the Avana Dataset (DepMap 20Q1)'>
Global Fraction of Cell Lines Where Essential: 739/739
^Tissue^Fraction Of Cell Lines Where Essential^
|1290807.0|1/1|
|909776.0|1/1|
|bile duct|28/28|
|blood|28/28|
|bone|26/26|
|breast|33/33|
|central nervous system|56/56|
|cervix|4/4|
|colorectal|17/17|
|esophagus|13/13|
|fibroblast|1/1|
|gastric|16/16|
|kidney|21/21|
|liver|20/20|
|lung|75/75|
|lymphocyte|16/16|
|ovary|26/26|
|pancreas|24/24|
|peripheral nervous system|16/16|
|plasma cell|15/15|
|prostate|1/1|
|skin|24/24|
|soft tissue|9/9|
|thyroid|2/2|
|upper aerodigestive|22/22|
|urinary tract|29/29|
|uterus|5/5|
</modal>

== Essentiality in NALM6  ==
    * **<color #00a2e8>Essentiality Rank</color>**: 22
    * **<color #00a2e8>Expression level (log2 read counts)</color>**: 9.67 
<button type='primary' size='small' modal='Dist_expr'>Expression Distribution</button>
<modal id='Dist_expr' size='lg' title='PRPF8 Expression in NALM6 Cells: 9.67'>
{{:chemogenomics:nalm6 dist.png?nolink |}}
</modal>