PRPF8

Entrez Summary: Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq, Jul 2008].
UniProt Summary: Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site. {ECO:0000269|PubMed:20595234, ECO:0000303|PubMed:15840809}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Bortezomib 0.05μM R00 exp7	-2.21
Hinokiflavone 12μM R08 exp487	-2.14
Vitamin-D3 40μM R08 exp536	-2.07
GSK626616 14μM R08 exp485	-2.03
Rotenone 0.07μM R08 exp431	-1.76
Sulforaphane 9μM R08 exp525	-1.76
ML-792 0.2μM R08 exp505	-1.74
Ammonium tetrathiomolybdate 10μM R08 exp448	-1.71
Momordin-Ic 10μM R08 exp506	1.71
BS-181 20μM R08 exp461	1.76
Ribavirin 10 to 15μM on day4 R04 exp160	1.78
Artemisinin 50μM R08 exp450	1.84
ZLN024 50μM R08 exp538	1.86
Cycloheximide 0.02μM R00 exp14	1.87
Methotrexate 0.01μM R08 exp501	1.93
Ixabepilone 0.004 to 0.005μM on day4 R04 exp184	2.03
CCCP 0.1μM R00 exp10	2.38
Nocodazole 0.02μM R00 exp23	2.4
PFI-3 10μM R03 exp144	2.56
Chloramphenicol 2μM R00 exp12	2.59
Selumetinib 20μM R02 exp81	2.81
Alpha-Amanitin 0.5μM R00 exp5	3

Global Fraction of Cell Lines Where Essential: 739/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	28/28
blood	28/28
bone	26/26
breast	33/33
central nervous system	56/56
cervix	4/4
colorectal	17/17
esophagus	13/13
fibroblast	1/1
gastric	16/16
kidney	21/21
liver	20/20
lung	75/75
lymphocyte	16/16
ovary	26/26
pancreas	24/24
peripheral nervous system	16/16
plasma cell	15/15
prostate	1/1
skin	24/24
soft tissue	9/9
thyroid	2/2
upper aerodigestive	22/22
urinary tract	29/29
uterus	5/5

Expression Distribution

U5 snRNA binding
second spliceosomal transesterification activity
U2 snRNA binding
U1 snRNA binding
pre-mRNA intronic binding
U6 snRNA binding
U2-type catalytic step 1 spliceosome
spliceosomal tri-snRNP complex assembly
U5 snRNP
K63-linked polyubiquitin modification-dependent protein binding
U2-type catalytic step 2 spliceosome
U4/U6 x U5 tri-snRNP complex
spliceosomal snRNP assembly
U2-type precatalytic spliceosome
catalytic step 2 spliceosome
cellular response to lipopolysaccharide
cellular response to molecule of bacterial origin
cellular response to biotic stimulus
ribonucleoprotein complex assembly
cellular response to tumor necrosis factor
ribonucleoprotein complex subunit organization
response to tumor necrosis factor
RNA splicing, via transesterification reactions with bulged adenosine as nucleophile
mRNA splicing, via spliceosome
RNA splicing, via transesterification reactions
response to lipopolysaccharide
response to molecule of bacterial origin
RNA splicing
nuclear speck
ribonucleoprotein complex biogenesis
mRNA processing
cellular response to lipid
response to bacterium
mRNA metabolic process
cellular protein-containing complex assembly
response to lipid
RNA processing
cellular response to cytokine stimulus
cellular response to oxygen-containing compound
response to cytokine