Cycloheximide 0.02μM R00 exp14
Mechanism of Action
Translation elongation inhibitor, binds 60S ribosomal subunit
- Class / Subclass 1: Proteostasis / Translation Inhibitor
- Class / Subclass 2: Environmental Stresses / Toxin
Technical Notes
Compound References
- PubChem Name: Cycloheximide
- Synonyms: Naramycin A; Actidione; CHX
- CAS #: 66-81-9
- PubChem CID: 6197
- IUPAC: 4-[(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl]piperidine-2,6-dione
- INCHI Name: InChI=1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1
- INCHI Key: YPHMISFOHDHNIV-FSZOTQKASA-N
- Molecular Weight: 281.35
- Canonical SMILES: CC1CC(C(=O)C(C1)C(CC2CC(=O)NC(=O)C2)O)C
- Isomeric SMILES: C[C@H]1C[C@@H](C(=O)[C@@H](C1)[C@@H](CC2CC(=O)NC(=O)C2)O)C
- Molecular Formula: C15H23NO4
Compound Supplier
- Supplier Name: Sigma-Aldrich
- Catalog #: C7698
- Lot #: MKBR7146V
Compound Characterization
- HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C15H23NO4 282.16998; found 282.17004
Dose Response Curve
- Platform ID: Cycloheximide
- Min: 3.7773; Max: 80.9065
| IC | Concentration (µM) |
|---|---|
| IC10 | N/A |
| IC20 | 0.1270 |
| IC30 | 0.3675 |
| IC40 | 0.8782 |
| IC50 | 1.9535 |
| IC60 | N/A |
| IC70 | N/A |
| IC80 | N/A |
| IC90 | N/A |
Screen Summary
- Round: 00
- Dose: 20nM
- Days of incubation: 8
- Doublings: 6.5
- Numbers of reads: 10420892
Screen Results
| Sensitive/Resistant hits (FDR<0.05) | CRANKS | Score Plot | Top 30 Genes | Screen Similarity | Top 30 Sensitive GO terms | Top 30 Resistant GO terms |
|---|---|---|---|---|---|---|
| 0/2 | Scores |
Cycloheximide 0.02μM R00 exp14
Cycloheximide 0.02μM R00 exp14 vs Nocodazole 0.02μM R00 exp23
Cycloheximide 0.02μM R00 exp14 vs Pimelic-diphenylamide-106 0.5μM R00 exp27
Cycloheximide 0.02μM R00 exp14 vs DABN 2μM R00 exp16
CCCP 1μM R00 exp11 vs Cycloheximide 0.02μM R00 exp14
