CUL2

Entrez Summary: N/A
UniProt Summary: Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). {ECO:0000269|PubMed:10973499, ECO:0000269|PubMed:11384984, ECO:0000269|PubMed:27565346, ECO:0000269|PubMed:9122164}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Rapamycin 2μM R07 exp306	-2.76
HMS-I1 1μM R01 exp46	-2.54
Nocodazole 0.02μM R00 exp23	-2.35
Arsenate 40μM R06 exp264	-2.24
FK-506 5μM R07 exp358	-2.21
Vinblastine 0.002μM R01 exp60	-2.19
Nicotinamide 2000μM R01 exp50	-2.08
Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53	-2.08
Mubritinib 0.2μM R01 exp48	-2.06
Pimelic-diphenylamide-106 0.5μM R00 exp27	-2.06
YM155 0.0002μM R01 exp61	-2.05
Cycloheximide 0.02μM R00 exp14	-2.02
Dimethyloxaloylglycine 11μM R07 exp314	-1.97
QNZ 0.01μM R05 exp241	-1.97
ABT-702 5μM R07 exp318	-1.96
Benzoate 20000μM R08 exp456	-1.94
IU1-C 25μM R04 exp183	-1.91
Pterostilbene 16μM R02 exp78	-1.91
EPZ-5676 30μM R05 exp233	-1.83
UM131593 0.2μM R03 exp106	-1.83
UMK57 1μM R01 exp59	-1.8
Apcin 25μM plus proTAME 2μM R04 exp177	-1.79
Taxol 0.002μM R01 exp54	-1.78
LLY-284 2.6μM R06 exp291	-1.77
MK-1775 0.32μM R02 exp75	-1.77
BAY-598 4μM R05 exp220	-1.76
Epothilone-B 0.0015μM R05 exp231	-1.76
A-395 10μM R05 exp218	-1.75
Vinorelbine 0.001μM R05 exp249	-1.73
Isoniazid 100μM R08 exp494	-1.71
SB743921 0.001μM R05 exp244	2.01

Global Fraction of Cell Lines Where Essential: 312/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	0/1
bile duct	13/28
blood	7/28
bone	12/26
breast	8/33
central nervous system	30/56
cervix	4/4
colorectal	2/17
esophagus	6/13
fibroblast	1/1
gastric	2/16
kidney	9/21
liver	9/20
lung	36/75
lymphocyte	5/16
ovary	13/26
pancreas	11/24
peripheral nervous system	13/16
plasma cell	5/15
prostate	0/1
skin	17/24
soft tissue	6/9
thyroid	0/2
upper aerodigestive	11/22
urinary tract	12/29
uterus	1/5

Expression Distribution

Cullin
Cullin Nedd8

VCB complex
cullin-RING ubiquitin ligase complex
Cul2-RING ubiquitin ligase complex
SCF ubiquitin ligase complex
regulation of transcription from RNA polymerase II promoter in response to hypoxia
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
regulation of transcription from RNA polymerase II promoter in response to stress
G1/S transition of mitotic cell cycle
cell cycle G1/S phase transition
regulation of DNA-templated transcription in response to stress
intrinsic apoptotic signaling pathway
cellular response to hypoxia
cellular response to decreased oxygen levels
cellular response to oxygen levels
protein-containing complex binding
mitotic cell cycle phase transition
cell cycle phase transition
apoptotic signaling pathway
ubiquitin protein ligase binding
proteasome-mediated ubiquitin-dependent protein catabolic process
response to hypoxia
proteasomal protein catabolic process
response to decreased oxygen levels
post-translational protein modification
response to oxygen levels
ubiquitin-dependent protein catabolic process
modification-dependent protein catabolic process
modification-dependent macromolecule catabolic process
proteolysis involved in cellular protein catabolic process
mitotic cell cycle process
cellular protein catabolic process
protein catabolic process
mitotic cell cycle
protein ubiquitination
viral process
protein modification by small protein conjugation
symbiotic process
interspecies interaction between organisms
nucleolus
cellular macromolecule catabolic process