Apcin 25μM plus proTAME 2μM R04 exp177

Inhibits substrate interactions with APC/C-Cdc20

Class / Subclass 1: Proteostasis / E3 ubiquitin ligase inhibitor
Class / Subclass 2: Cell Cycle / Mitotic Inhibitor

Apcin

Compound References

PubChem Name: Apcin
Synonyms: N/A
CAS #: 300815-04-7
PubChem CID: 2830389
IUPAC: 2-(2-methyl-5-nitroimidazol-1-yl)ethyl N-[2,2,2-trichloro-1-(pyrimidin-2-ylamino)ethyl]carbamate
INCHI Name: InChI=1S/C13H14Cl3N7O4/c1-8-19-7-9(23(25)26)22(8)5-6-27-12(24)21-10(13(14,15)16)20-11-17-3-2-4-18-11/h2-4,7,10H,5-6H2,1H3,(H,21,24)(H,17,18,20)
INCHI Key: ZEXHXVOGJFGTRX-UHFFFAOYSA-N
Molecular Weight: 438.6
Canonical SMILES: CC1=NC=C(N1CCOC(=O)NC(C(Cl)(Cl)Cl)NC2=NC=CC=N2)[N+](=O)[O-]
Isomeric SMILES: N/A
Molecular Formula: C13H14Cl3N7O4

Compound Supplier

Supplier Name: Boston Biochem
Catalog #: I-444
Lot #: N/A

Compound Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C13H14Cl3N7O4 438.02456; found 438.02545

proTAME

Compound References

PubChem Name: Methyl (2S)-5-[bis[(2-phenylacetyl)oxymethoxycarbonylamino]methylideneamino]-2-[(4-methylphenyl)sulfonylamino]pentanoate
Synonyms: N/A
CAS #: 1362911-19-0
PubChem CID: 56924780
IUPAC: methyl (2S)-5-[bis[(2-phenylacetyl)oxymethoxycarbonylamino]methylideneamino]-2-[(4-methylphenyl)sulfonylamino]pentanoate
INCHI Name: InChI=1S/C34H38N4O12S/c1-24-15-17-27(18-16-24)51(44,45)38-28(31(41)46-2)14-9-19-35-32(36-33(42)49-22-47-29(39)20-25-10-5-3-6-11-25)37-34(43)50-23-48-30(40)21-26-12-7-4-8-13-26/h3-8,10-13,15-18,28,38H,9,14,19-23H2,1-2H3,(H2,35,36,37,42,43)/t28-/m0/s1
INCHI Key: MHYOVHULCQSDRZ-NDEPHWFRSA-N
Molecular Weight: 726.8
Canonical SMILES: CC1=CC=C(C=C1)S(=O)(=O)NC(CCCN=C(NC(=O)OCOC(=O)CC2=CC=CC=C2)NC(=O)OCOC(=O)CC3=CC=CC=C3)C(=O)OC
Isomeric SMILES: CC1=CC=C(C=C1)S(=O)(=O)N[C@@H](CCCN=C(NC(=O)OCOC(=O)CC2=CC=CC=C2)NC(=O)OCOC(=O)CC3=CC=CC=C3)C(=O)OC
Molecular Formula: C34H38N4O12S

Compound Supplier

Supplier Name: Boston Biochem
Catalog #: I-440
Lot #: N/A

Compound Characterization

LCMS: Tr 1.24 min, m/z 203-

Apcin+proTAME

Dose Response Curve

Platform ID: Apcin_25uM+proTAME
Min: -20.0516; Max: 70.3376

IC	Concentration (µM)
IC10	N/A
IC20	3.9640
IC30	4.7857
IC40	5.5848
IC50	6.4350
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

The dose response curve for Apcin is also available at:

exp176

The dose response curve for proTAME is also available at:

exp187

Screen Summary

Round: 04
Dose: 25µM + 2µM
Days of incubation: 8
Doublings: 6.0
Numbers of reads: 26092227

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
15/6	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
TRIP13	-3.74	<0.01
ATP2B1	-3.62	<0.01
ANAPC15	-3.50	<0.01
SLC18B1	-3.44	<0.01
UBE2C	-3.36	<0.01
UBE2S	-3.24	<0.01
MAD2L1BP	-2.95	<0.01
LAMTOR4	-2.85	<0.01
RSRC2	-2.83	0.06
RAB7A	-2.74	<0.01
ARID4B	-2.71	0.02
TCF4	-2.58	0.02
ANAPC7	-2.58	0.02
RBM15	-2.52	0.02
PTAR1	-2.52	0.05
ANAPC13	-2.50	0.05
ARL1	-2.45	0.03
SNUPN	-2.43	0.06
DNAJC8	-2.40	0.05
DPAGT1	-2.33	0.06
SLC5A6	-2.30	0.06
THOP1	-2.29	0.06
ABCC1	-2.28	0.06
CCNB1	-2.18	0.15
THG1L	-2.17	0.11
ATP6V1G1	-2.16	0.16
ADSL	-2.16	0.16
C16orf86	-2.15	0.12
SMLR1	-2.14	0.12
KDSR	-2.13	0.12
ILDR2	2.02	0.38
DGCR14	2.03	0.42
EXOSC3	2.04	0.42
ALAD	2.04	0.42
DEFB110	2.05	0.69
SLC4A1	2.06	0.69
PAK4	2.06	0.42
TSPY8	2.08	0.77
NARFL	2.09	0.65
FNDC3A	2.12	0.23
FAM172A	2.12	0.23
POLR2J2	2.12	0.77
SLC5A3	2.12	0.23
NUP43	2.13	0.48
DHX38	2.17	0.62
RNH1	2.20	0.21
GPATCH8	2.21	0.21
DDX47	2.26	0.77
AOC2	2.26	0.23
ABHD17B	2.30	0.14
CDC14A	2.39	0.11
MDH1	2.46	0.14
POLR2J3	2.47	0.77
RBP4	2.57	0.04
RAB10	2.63	0.04
NCEH1	2.85	0.01
NOC3L	2.87	0.23
ZNF653	3.21	<0.01
CCNB2	3.44	<0.01
ABHD6	5.87	<0.01

Screen	Correlation	Plot
proTAME 5μM R04 exp187	0.148	View
Apcin 50 to 100μM on day4 R04 exp176	0.094	View
Vincristine 0.0005μM R04 exp190	0.06	View
UM0130462 0.025 to 0.035μM day4 R05 exp247	0.057	View
RO-3306 3 to 4μM on day4 R04 exp165	0.055	View

GO Term	Fold Change	Genes
anaphase-promoting complex	159.37	ANAPC15,UBE2C,UBE2S,ANAPC7,ANAPC13
anaphase-promoting complex-dependent catabolic process	159.37	ANAPC15,UBE2C,UBE2S,ANAPC7,ANAPC13
positive regulation of mitotic metaphase/anaphase transition	147.11	UBE2C,MAD2L1BP,ANAPC7
positive regulation of metaphase/anaphase transition of cell cycle	136.60	UBE2C,MAD2L1BP,ANAPC7
positive regulation of mitotic sister chromatid separation	112.49	UBE2C,MAD2L1BP,ANAPC7
regulation of spindle checkpoint	95.62	ANAPC15,MAD2L1BP,CCNB1
protein K11-linked ubiquitination	91.07	UBE2C,UBE2S,ANAPC7,ANAPC13
nuclear ubiquitin ligase complex	74.12	ANAPC15,UBE2C,UBE2S,ANAPC7,ANAPC13
regulation of mitotic sister chromatid separation	65.94	TRIP13,ANAPC15,UBE2C,MAD2L1BP,ANAPC7,CCNB1
regulation of chromosome separation	52.39	TRIP13,ANAPC15,UBE2C,MAD2L1BP,ANAPC7,CCNB1
regulation of mitotic sister chromatid segregation	48.11	TRIP13,ANAPC15,MAD2L1BP,CCNB1
regulation of mitotic metaphase/anaphase transition	42.97	TRIP13,ANAPC15,UBE2C,MAD2L1BP,ANAPC7,CCNB1
regulation of metaphase/anaphase transition of cell cycle	41.57	TRIP13,ANAPC15,UBE2C,MAD2L1BP,ANAPC7,CCNB1
regulation of meiotic cell cycle	40.47	TRIP13,ANAPC15,ANAPC7,ANAPC13
regulation of sister chromatid segregation	37.13	TRIP13,ANAPC15,UBE2C,MAD2L1BP,ANAPC7,CCNB1
regulation of mitotic nuclear division	32.69	TRIP13,ANAPC15,UBE2C,MAD2L1BP,ANAPC7,CCNB1
regulation of chromosome segregation	30.36	TRIP13,ANAPC15,UBE2C,MAD2L1BP,ANAPC7,CCNB1
regulation of nuclear division	26.56	TRIP13,ANAPC15,UBE2C,MAD2L1BP,ANAPC7,CCNB1
regulation of chromosome organization	15.80	TRIP13,ANAPC15,UBE2C,MAD2L1BP,ANAPC7,CCNB1

Apcin 25μM plus proTAME 2μM R04 exp177

Mechanism of Action

Technical Notes

Apcin

Compound References

Compound Supplier

Compound Characterization

proTAME

Compound References

Compound Supplier

Compound Characterization

Apcin+proTAME

Dose Response Curve

Screen Summary

Screen Results