Vincristine 0.0005μM R04 exp190

Inhibits microtubule polymerization and mitotic spindle formation, induces spindle assembly checkpoint (SAC)

PubChem Name: Vincristine sulfate
Synonyms: Leurocristine sulfate; 22-Oxovincaleukoblastine sulfate
CAS #: 2068-78-2
PubChem CID: 249332
IUPAC: methyl (1R,9R,10S,11R,12R,19R)-11-acetyloxy-12-ethyl-4-[(13S,15S,17S)-17-ethyl-17-hydroxy-13-methoxycarbonyl-1,11-diazatetracyclo[13.3.1.04,12.05,10]nonadeca-4(12),5,7,9-tetraen-13-yl]-8-formyl-10-hydroxy-5-methoxy-8,16-diazapentacyclo[10.6.1.01,9.02,7.016,19]nonadeca-2,4,6,13-tetraene-10-carboxylate;sulfuric acid
INCHI Name: InChI=1S/C46H56N4O10.H2O4S/c1-7-42(55)22-28-23-45(40(53)58-5,36-30(14-18-48(24-28)25-42)29-12-9-10-13-33(29)47-36)32-20-31-34(21-35(32)57-4)50(26-51)38-44(31)16-19-49-17-11-15-43(8-2,37(44)49)39(60-27(3)52)46(38,56)41(54)59-6;1-5(2,3)4/h9-13,15,20-21,26,28,37-39,47,55-56H,7-8,14,16-19,22-25H2,1-6H3;(H2,1,2,3,4)/t28-,37+,38-,39-,42+,43-,44-,45+,46+;/m1./s1
INCHI Key: AQTQHPDCURKLKT-JKDPCDLQSA-N
Molecular Weight: 923
Canonical SMILES: CCC1(CC2CC(C3=C(CCN(C2)C1)C4=CC=CC=C4N3)(C5=C(C=C6C(=C5)C78CCN9C7C(C=CC9)(C(C(C8N6C=O)(C(=O)OC)O)OC(=O)C)CC)OC)C(=O)OC)O.OS(=O)(=O)O
Isomeric SMILES: CC[C@@]1(C[C@@H]2C[C@@](C3=C(CCN(C2)C1)C4=CC=CC=C4N3)(C5=C(C=C6C(=C5)[C@]78CCN9[C@H]7[C@@](C=CC9)([C@H]([C@@]([C@@H]8N6C=O)(C(=O)OC)O)OC(=O)C)CC)OC)C(=O)OC)O.OS(=O)(=O)O
Molecular Formula: C46H58N4O14S

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
56/22	Scores	View	View	View	View	View

Screen	Correlation	Plot
Combretastatin A4 0.002 to 0.003μM day4 R04 exp179	0.12	View
Ixabepilone 0.004 to 0.005μM on day4 R04 exp184	0.117	View
BML-284 0.09μM R08 exp460	0.116	View
Demecolcine 0.03μM R05 exp228	0.113	View
Colchicine 0.009μM R05 exp215	0.112	View
Mdivi-1 15μM R05 exp217	0.097	View
2-Methoxyestradiol 0.55μM R07 exp310	0.089	View
2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312	0.087	View
Quercetin 20μM R08 exp517	0.086	View
Taxol 0.004μM R03 exp103	0.084	View
Vinblastine 0.002μM R01 exp60	0.081	View
S-trityl-L-cysteine 0.5μM R05 exp243	0.079	View
Docetaxel 0.001 to 0.002μM on day4 R04 exp159	0.076	View
Docetaxel 0.002μM R01 exp45	0.073	View
UMK57 1μM R01 exp59	0.07	View
GSK461364A 0.005μM R03 exp125	0.07	View
Sulforaphane 9μM R08 exp525	0.065	View
Sulforaphane 9μM R08 exp526 no dilution day6	0.061	View
Epothilone-B 0.0015μM R05 exp231	0.06	View
Apcin 25μM plus proTAME 2μM R04 exp177	0.06	View
2-Methoxyestradiol 0.55 to 0.75μM on day4 R07 exp311	0.06	View
BI-6727 0.0125μM R03 exp97	0.06	View
LB-100 2μM R05 exp210	0.059	View
Cabazitaxel 0.001μM R05 exp223	0.057	View
Menadione 5μM R06 exp292	0.057	View
ETC-159 50μM R08 exp480	0.057	View
BI-2536 0.0042μM R03 exp95	0.053	View
Pyridostatin 4μM R05 exp240	0.053	View
UMK57 0.6μM R03 exp107	0.051	View

GO Term	Fold Change	Genes
chromosome localization	25.85	MAPRE1,DLGAP5,KIFC1,CENPF
spindle assembly	22.53	MAPRE1,ASPM,KIFC1,HDAC3
spindle pole	18.05	MAPRE1,ASPM,DLGAP5,CALM2,CENPF
spindle organization	17.74	MAPRE1,ASPM,DLGAP5,KIFC1,HDAC3
mitotic spindle	17.24	MAPRE1,ASPM,DLGAP5,KIFC1,HDAC3
nuclear chromosome segregation	13.95	MAPRE1,ASPM,DLGAP5,KIFC1,CENPF
spindle	13.31	MAPRE1,ASPM,DLGAP5,KIF15,CALM2,KIFC1,ZWILCH,HDAC3,CENPF
chromosome segregation	11.23	MAPRE1,ASPM,DLGAP5,KIFC1,HDAC3,CENPF
non-membrane-bounded organelle assembly	10.96	MAPRE1,ASPM,DLGAP5,KIFC1,HDAC3,CENPF
tubulin binding	10.09	MAPRE1,DLGAP5,KIF15,KIFC1,CENPF,VBP1
mitotic cell cycle process	8.31	MAPRE1,DLGAP5,CALM2,KIFC1,ZWILCH,HDAC3,CENPF
organelle localization	8.20	MAPRE1,ASPM,TMEM230,DLGAP5,KIFC1,HDAC3,CENPF
mitotic cell cycle	8.17	MAPRE1,DLGAP5,KIF15,CALM2,KIFC1,ZWILCH,HDAC3,CENPF

GO Term	Fold Change	Genes
translation initiation factor activity	45.10	EIF4A1,EIF4G2,EIF3H,EIF3G
translational initiation	42.50	DDX3X,EIF4A1,EIF4G2,EIF3H,EIF3G