DPAGT1

Gene Information

Official Symbol: DPAGT1
Official Name: dolichyl-phosphate N-acetylglucosaminephosphotransferase 1
Aliases and Previous Symbols: N/A
Entrez ID: 1798
UniProt: Q9H3H5
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: The protein encoded by this gene is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. This enzyme belongs to the glycosyltransferase family 4. This protein is an integral membrane protein of the endoplasmic reticulum. The congenital disorder of glycosylation type Ij is caused by mutation in the gene encoding this enzyme. [provided by RefSeq, Jul 2008].
UniProt Summary: Catalyzes the initial step in the synthesis of dolichol- P-P-oligosaccharides.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Tunicamycin 0.04 to 0.125μM on day4 R07 exp420	-4.4
Tunicamycin 0.04μM R07 exp418	-4.23
Tunicamycin 0.04 to 0.075μM on day4 R07 exp419	-3.67
Asunaprenir 3μM R07 exp336	-3.41
A-395 10μM R05 exp218	-3.36
Chloroquine 32μM R08 exp470	-3.13
PFI-3 10μM R03 exp144	-2.78
Ethanol 0.01 R05 exp234	-2.75
FTY720 3μM R08 exp483	-2.7
Cannabidiol 20μM R08 exp466	-2.67
Rapamycin 0.001μM R08 exp429	-2.67
Cerivastatin 0.15μM R05 exp226	-2.61
Ethanol 0.003 R08 exp437	-2.58
VE-822 0.04μM R06 exp300	-2.56
GSK-J5 1.5μM R08 exp484	-2.55
IL-3 9ng/ml R08 exp493	-2.54
A-196 10μM R03 exp114	-2.53
MS094 2μM R03 exp136	-2.52
Resminostat 0.5μM R07 exp393	-2.5
SGC0649 7μM R03 exp150	-2.45
Cabazitaxel 0.001μM R05 exp223	-2.44
ZLN024 50μM R08 exp538	-2.44
Azithromycin 100μM R08 exp452	-2.4
BS-181 20μM R08 exp461	-2.38
VHL-ligand-1 20μM R07 exp421	-2.35
Trientine 500μM R08 exp534	-2.34
Apcin 25μM plus proTAME 2μM R04 exp177	-2.33
Golgicide-A 2μM R03 exp121	-2.31
Phorbol-12-myristate-13-acetate 0.57μM R08 exp514	-2.31
GSK-LSD1 10μM R03 exp123	-2.27
Heregulin-B 44ng/ml R08 exp486	-2.26
Dihydrosphingosine 8μM R08 exp476	-2.24
Cincreasin 100μM R08 exp473	-2.22
LB-100 2μM R05 exp210	-2.22
NN-Diethyl-meta-toluamide 500μM R08 exp438	-2.21
HMS-I2 10μM R06 exp288	-2.18
All-trans-Retinoic-Acid 40μM R07 exp334	-2.13
Chlorpromazine 10μM R07 exp344	-2.13
Epigallocatechin gallate 20μM R05 exp230	-2.12
Bisphenol F 50μM R08 exp457	-2.1
B02 10μM R08 exp453	-2.08
Fluvastatin 2.2μM R06 exp282	-2.08
Trametinib 10μM R02 exp83	-2.07
Nicotinamide 1000μM R03 exp101	-2.06
NFN1 1μM R03 exp100	-2.04
DABN 2μM R00 exp16	-2.03
A-395N 10μM R05 exp219	-2.02
Parthenolide 2.5μM R05 exp238	-2.01
Dexamethasone 0.006μM R07 exp351	-2
GSK343 3μM R03 exp124	-1.98
Dynasore 10μM R04 exp180	-1.97
YM155 0.001μM R08 exp432	-1.97
Zebularine 20μM R07 exp423	-1.96
Bisphenol S 100μM R08 exp458	-1.91
Hydroxychloroquine 30μM R08 exp490	-1.91
L-BMAA 500 to 750μM on day4 R04 exp185	-1.91
Cisplatin 0.35μM R06 exp273	-1.9
NN-Dimethylsphingosine 2.5μM R08 exp508	-1.9
GSK591 2.6μM R03 exp128	-1.9
Pyrazinamide 100μM R08 exp516	-1.89
Cannabidiol 13μM R08 exp465	-1.88
Methotrexate 0.01μM R08 exp501	-1.88
Ethambutol 25μM R08 exp481	-1.87
Lenalidomide 20μM R07 exp375	-1.87
Ribavirin 10μM R01 exp52	-1.87
UM0011500 5μM R05 exp245	-1.87
S-DABN 8μM R03 exp112	-1.86
LLY-284 2.6μM R06 exp291	-1.84
NFN1 0.4μM R03 exp99	-1.84
Ibrutinib 1μM R07 exp372	-1.83
IU1-47 25μM R04 exp182	-1.83
Adefovir 20μM R07 exp332	-1.83
RS-1 10μM R08 exp519	-1.83
35°C R06 exp302	-1.81
A-366 10μM R03 exp115	-1.8
ABT-702 0.1μM R06 exp260	-1.8
Deguelin 0.05μM R02 exp69	-1.8
Dimethyl-Sulfoxide 1pc R03 exp120	-1.8
BRD2 inhibitor II 20μM R07 exp341	-1.79
Metformin 40μM R03 exp132	-1.79
Thioridazine 5μM R08 exp530	-1.79
Nicotinamide Riboside 100μM R03 exp139	-1.78
SGC2096 2.6μM R03 exp153	-1.78
NFN1 0.1μM R01 exp49	-1.77
Nocodazole 0.02μM R00 exp23	-1.76
LJH685 50μM R08 exp433	-1.74
Sucrose 20000μM R06 exp298	-1.74
Taxol 0.002μM R01 exp54	-1.74
Beta-Estradiol 0.55μM R07 exp425	-1.71
Fas-L 44ng/ml R08 exp482	-1.71
PT-1 25μM R07 exp392	-1.71
proTAME 5μM R04 exp187	-1.7
Salinomycin 3.5μM R08 exp521	1.9
GSK-J4 1.5μM R08 exp441	1.92
Atovaquone 15μM R08 exp451	1.94
CFI-400945 25μM R08 exp469	1.98
Ammonium tetrathiomolybdate 10μM R08 exp448	2.03
HMS-I1 10μM R06 exp256	2.04
Nocodazole 0.2μM R02 exp64	2.08
ONC212 0.15μM R08 exp513	2.21
42°C R08 exp539	2.24
PU-H71 1μM R08 exp515	2.24
R-DABN 8μM R03 exp111	2.36
BI-6727 0.04μM R03 exp98	2.37
CI-1040 9.5μM R08 exp472	2.46
Hinokiflavone 12μM R08 exp487	2.57
BI-2536 0.02μM R03 exp96	2.63
TIC10 10μM R08 exp532	2.69
Nocodazole 0.1μM R03 exp94	3.01
GSK461364A 0.1μM R03 exp126	3.12
DABN racemic mixture R03 exp93	3.14
Vinblastine 0.2μM R03 exp108	3.28

Gene	Correlation
PMM2	0.763
THG1L	0.76
ALG1	0.749
GFPT1	0.736
ATXN10	0.721
UAP1	0.708
RTCB	0.704
TRIAP1	0.7
GMPPB	0.691
RNMT	0.666
MED16	0.664
RPTOR	0.663
DOLK	0.657
DOHH	0.656
ALG2	0.639
SRP14	0.637
ALG13	0.622
ADSL	0.613
CCDC101	0.613
ALG5	0.61
SRD5A3	0.607
VEZT	0.607
UHRF1	0.603
ORAOV1	0.603
DAD1	0.602
ENY2	0.602
ALG6	0.6
BAP1	0.596
ROMO1	0.594
RPE	0.594
RPP21	0.594
GNB1L	0.59
GTF3C2	0.59
GTF3C4	0.589
HSPD1	0.588
GTF3C3	0.588
PPCS	0.584
TMEM41B	0.584
TTI2	0.581
PELO	0.58
TRMT5	0.577
PAM16	0.576
ATP5J	0.576
ALG14	0.575
UTP23	0.574
TSEN54	0.574
NAE1	0.574
TTC4	0.573
MBTPS2	0.573
TBCA	0.571
CBFB	0.57
TRNT1	0.57
POLR3H	0.563
MED4	0.561
CSNK2B	0.561
TTI1	0.56
DHPS	0.558
GTF3C6	0.554
EXOC4	0.554
ATP5B	0.552
EIF4E	0.552
GTF2A2	0.546
DHRSX	0.544
AASDHPPT	0.542
HCCS	0.541
TAMM41	0.541
AARS	0.539
SUPV3L1	0.538
RPIA	0.537
ELAC2	0.537
DDX20	0.536
ZRSR2	0.536
USO1	0.535
LAMTOR5	0.534
UBE2G2	0.534
SLC25A26	0.533
ATP5O	0.532
FH	0.529
WDR59	0.529
TRMT10A	0.527
ATP2A2	0.525
CDIPT	0.525
FNTA	0.524
VMP1	0.524
ATIC	0.524
PPA2	0.523
WDR24	0.523
EPRS	0.522
TELO2	0.521
TIMM22	0.52
CRLS1	0.52
TAZ	0.52
SLC35B1	0.519
GEMIN8	0.517
THOC6	0.516
SRP72	0.515
RAD1	0.515
MLLT1	0.515
RFT1	0.515
OSGEP	0.513
PPP2R3C	0.512
UBA3	0.512
TPRKB	0.511
YARS	0.511
GEMIN5	0.509
BUB3	0.508
CTDNEP1	0.507
RABGGTB	0.507
SEC62	0.505
CEP57	0.505
LSM11	0.504
DDX1	0.504
PPP4C	0.503
LSM10	0.502
CHCHD4	0.501
RPN1	0.5
UXT	0.499
GEMIN6	0.499
MEAF6	0.499
TSEN34	0.498
MTOR	0.497
NARS	0.497
SSB	0.495
POLR3C	0.495
CASP9	0.494
ENO1	0.494
VRK1	0.494
EEF1E1	0.494
DNAJC3	0.494
PDAP1	0.493
DR1	0.493
RBM14	0.493
PYROXD1	0.492
NSMCE1	0.492
HAUS8	0.491
LSMD1	0.487
HSD17B10	0.487
POLR3B	0.486
SRP68	0.484
SLC25A19	0.484
MRPS33	0.482
TGS1	0.482
CCT7	0.481
URM1	0.481
TIMM13	0.48
WDR92	0.479
TBP	0.479
TBCB	0.478
MED23	0.477
BCS1L	0.477
KDSR	0.477
ELP3	0.477
TEN1	0.476
DDOST	0.476
WDR82	0.475
SDHA	0.475
PPP6C	0.473
DARS2	0.472
SEC63	0.471
DHDDS	0.471
TMED10	0.47
APAF1	0.47
PLAA	0.469
MAD2L1	0.469
MAPK14	0.468
CBX3	0.468
DPF2	0.467
MED25	0.465
TPK1	0.464
SPCS2	0.464
UBE2J1	0.462
MRPS14	0.461
PGM3	0.459
TKT	0.459
SRPRB	0.459
PGGT1B	0.458
IRF2BP2	0.457
OTUD5	0.456
FGFR1OP	0.456
CRCP	0.455
NSD1	0.455
MBTPS1	0.455
ALG9	0.455
ZBTB11	0.454
GFER	0.454
KIAA0391	0.454
ATP5A1	0.453
ATP5H	0.453
THOC3	0.452
COX16	0.452
ATP5D	0.452
YARS2	0.452
PDSS1	0.452
FNTB	0.451
RFK	0.449
VMA21	0.448
UBE2K	0.448
DNLZ	0.447
TXNL4B	0.446
TXNRD1	0.446
DHX29	0.445
ELP2	0.442
OIP5	0.441
MRPS6	0.439
PHB	0.439
GGNBP2	0.439
ACTR10	0.438
MRPS11	0.438
MARS	0.437
VHL	0.436
DDX55	0.436
SEC16A	0.436
ERCC2	0.434
PIK3R4	0.434
GEMIN7	0.434
ZNF259	0.434
ALG8	0.434
EXOC3	0.434
GPN2	0.433
QARS	0.433
TRMT61A	0.433
C10orf2	0.433
FAF2	0.433
SNAPC5	0.432
EIF1AX	0.432
UBE3A	0.432
UQCRC2	0.432
SEC61G	0.431
GTF3A	0.43
TUFM	0.43
CMTR1	0.429
FLI1	0.428
DDX39A	0.427
XPO6	0.427
OPA1	0.426
NAT9	0.425
NUPL2	0.425
MRPS16	0.425
SARS2	0.425
MIS18A	0.424
BAK1	0.424
C17orf89	0.422
SRRD	0.422
MRPS18A	0.421
GFM1	0.421
DERL2	0.421
STT3A	0.421
MRPL23	0.42
SOX7	0.419
SNF8	0.419
LMO2	0.418
EIF3F	0.418
TAF1A	0.417
DPM3	0.417
WARS	0.417
C14orf80	0.417
DCTN6	0.416
SSBP2	0.416
CCDC115	0.416
MAX	0.414
UBA5	0.414
FBXO11	0.413
TCF4	0.413
GTF3C5	0.413
TCEB3	0.412
ID3	0.412
SPCS3	0.411
MANF	0.411
CYCS	0.411
SERF2	0.411
KDM8	0.41
ATF7IP	0.409
UBQLN1	0.409
RPUSD4	0.408
C15orf41	0.407
PAX5	0.407
MAML1	0.406
LAMTOR4	0.405
PTDSS1	0.405
DERL1	0.404
ZZZ3	0.404
NBN	0.403
CARS	0.403
ZFX	0.403
SEH1L	0.402
TOMM40	0.402
RAD50	0.401
VPS26A	0.401
TRIM28	0.401
FEN1	0.401
MRPS34	0.4
C14orf166	0.4
TIMM10	0.4
MRPS26	0.4

Global Fraction of Cell Lines Where Essential: 730/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	28/28
blood	27/28
bone	26/26
breast	30/33
central nervous system	56/56
cervix	4/4
colorectal	17/17
esophagus	13/13
fibroblast	1/1
gastric	16/16
kidney	21/21
liver	20/20
lung	74/75
lymphocyte	15/16
ovary	26/26
pancreas	24/24
peripheral nervous system	15/16
plasma cell	15/15
prostate	1/1
skin	24/24
soft tissue	9/9
thyroid	2/2
upper aerodigestive	22/22
urinary tract	28/29
uterus	5/5

Essentiality in NALM6

Essentiality Rank: 267
Expression level (log2 read counts): 5.98

Expression Distribution

phospho-N-acetylmuramoyl-pentapeptide-transferase activity
UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity
UDP-N-acetylglucosamine-lysosomal-enzyme N-acetylglucosaminephosphotransferase activity
dolichol metabolic process
dolichyl diphosphate metabolic process
dolichyl diphosphate biosynthetic process
polyprenol metabolic process
UDP-N-acetylglucosamine metabolic process
transferase activity, transferring glycosyl groups
dolichol-linked oligosaccharide biosynthetic process
oligosaccharide-lipid intermediate biosynthetic process
nucleotide-sugar metabolic process
amino sugar metabolic process
protein N-linked glycosylation
integral component of endoplasmic reticulum membrane
isoprenoid metabolic process
phospholipid biosynthetic process
protein glycosylation
macromolecule glycosylation
glycosylation
alcohol metabolic process
glycoprotein biosynthetic process
phospholipid metabolic process
glycoprotein metabolic process
organic hydroxy compound metabolic process
protein complex oligomerization
organophosphate biosynthetic process
nucleobase-containing small molecule metabolic process
lipid biosynthetic process
carbohydrate derivative biosynthetic process
intracellular membrane-bounded organelle
organophosphate metabolic process
endoplasmic reticulum membrane
cellular lipid metabolic process
carbohydrate derivative metabolic process
lipid metabolic process
organonitrogen compound biosynthetic process
protein-containing complex assembly
cellular macromolecule biosynthetic process
small molecule metabolic process