TGS1

Entrez Summary: N/A
UniProt Summary: Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation. {ECO:0000269|PubMed:11517327, ECO:0000269|PubMed:11912212, ECO:0000269|PubMed:16687569, ECO:0000269|PubMed:18775984}.

Pfam Domains GO Terms

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
PF-06409577 20μM R07 exp389	-2.23
Suberoylanilide-Hydroxamic-Acid 0.02μM R01 exp53	-2.08
Zebularine 20μM R07 exp423	-1.97
Oligomycin-A 2μM R00 exp25	-1.93
ZLN024 50μM R08 exp538	-1.92
VE-822 0.04μM R06 exp300	-1.83
IWR1 50μM R08 exp495	-1.76
HMS-I2 5μM R06 exp287	-1.72
LY2090314 0.003μM R08 exp499	-1.72
DABN racemic mixture R03 exp93	1.74
MLN-4924 2μM R00 exp22	1.83
Etoposide 0.3μM R05 exp199	2.31

Global Fraction of Cell Lines Where Essential: 128/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	4/28
blood	1/28
bone	4/26
breast	7/33
central nervous system	5/56
cervix	0/4
colorectal	6/17
esophagus	1/13
fibroblast	0/1
gastric	3/16
kidney	4/21
liver	3/20
lung	15/75
lymphocyte	6/16
ovary	2/26
pancreas	2/24
peripheral nervous system	4/16
plasma cell	5/15
prostate	1/1
skin	1/24
soft tissue	2/9
thyroid	0/2
upper aerodigestive	5/22
urinary tract	7/29
uterus	1/5

Expression Distribution

RNA trimethylguanosine synthase activity
7-methylguanosine cap hypermethylation
RNA methyltransferase activity
small nuclear ribonucleoprotein complex
7-methylguanosine RNA capping
RNA capping
spliceosomal snRNP assembly
Cajal body
RNA methylation
RNA modification
ribonucleoprotein complex assembly
ribonucleoprotein complex subunit organization
macromolecule methylation
RNA splicing, via transesterification reactions with bulged adenosine as nucleophile
mRNA splicing, via spliceosome
RNA splicing, via transesterification reactions
methylation
regulation of lipid metabolic process
RNA splicing
ribonucleoprotein complex biogenesis
mRNA processing
mRNA metabolic process
cellular protein-containing complex assembly
nucleolus
RNA processing
protein-containing complex assembly
extracellular space
RNA metabolic process
protein-containing complex subunit organization
gene expression