RTCB

Gene Information

Official Symbol: RTCB
Official Name: RNA 2',3'-cyclic phosphate and 5'-OH ligase
Aliases and Previous Symbols: N/A
Entrez ID: 51493
UniProt: Q9Y3I0
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'- phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs. {ECO:0000269|PubMed:21311021, ECO:0000269|PubMed:24870230}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Lenalidomide 20μM R07 exp375	-2.57
VHL-ligand-1 20μM R07 exp421	-2.48
Phorbol-12-myristate-13-acetate 0.57μM R08 exp514	-2.44
BRD2 inhibitor II 20μM R07 exp341	-2.23
L-BMAA 500 to 750μM on day4 R04 exp185	-2.19
PFI-2 4μM R05 exp239	-2.12
BML-284 0.09μM R08 exp460	-2.11
Tunicamycin 0.04 to 0.075μM on day4 R07 exp419	-2.11
Ribavirin 10 to 15μM on day4 R04 exp160	-2.09
All-trans-Retinoic-Acid 8μM R07 exp333	-2.07
Cyclosporin-A 0.8μM R07 exp347	-2.07
Apcin 25μM plus proTAME 2μM R04 exp177	-2.06
UM0130462 0.025 to 0.035μM day4 R05 exp247	-2.04
Glyphosate 1000μM R06 exp283	-2.02
BN82002 4μM R07 exp340	-2.01
Hydroxychloroquine 30μM R08 exp490	-1.99
Dihydrosphingosine 8μM R08 exp476	-1.94
Cycloheximide 0.02μM R00 exp14	-1.93
TGF-beta1 44ng/ml R08 exp528	-1.93
Chlorpromazine 10μM R07 exp344	-1.89
Ixabepilone 0.004 to 0.005μM on day4 R04 exp184	-1.89
Pimelic-diphenylamide-106 0.5μM R00 exp27	-1.88
Pyrazinamide 100μM R08 exp516	-1.87
2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312	-1.86
Ibrutinib 1μM R07 exp372	-1.86
Adefovir 20μM R07 exp332	-1.85
Cincreasin 100μM R08 exp473	-1.82
Rucaparib 6.5μM R08 exp520	-1.81
Benzoate 10000μM R08 exp455	-1.8
Tunicamycin 0.04μM R07 exp418	-1.8
CoCl2 18μM R07 exp346	-1.77
Tenofovir 10μM R07 exp405	-1.77
Calcium Ionophore 0.4μM R07 exp342	-1.76
UM0131023 0.05μM R05 exp248	-1.76
Isoniazid 100μM R08 exp494	-1.75
PF-06409577 20μM R07 exp389	-1.75
Cadmium 60μM R08 exp462	-1.74
BI-6727 0.001μM R01 exp42	-1.72
Rapamycin 2μM plus FK-506 5μM R07 exp308	-1.72
Rapamycin 2μM R07 exp306	-1.72
Thioridazine 5μM R08 exp530	-1.71
Asunaprenir 3μM R07 exp336	-1.7
Chloramphenicol 2μM R00 exp12	-1.7
Atovaquone 15μM R08 exp451	1.7
SB743921 0.001μM R05 exp244	1.7
Cannabidiol 20μM R08 exp466	1.75
GSK-J4 1.5μM R08 exp441	1.76
SNS-032 15μM R08 exp443	1.76
SNS-032 25μM R07 exp401	1.86
Doxorubicin 0.02μM R08 exp478	1.93
Pimelic-diphenylamide-106 5μM R00 exp28	1.96
CI-1040 9.5μM R08 exp472	2.09
PU-H71 1μM R08 exp515	2.11
Nocodazole 0.1μM R03 exp94	2.14
R-DABN 8μM R03 exp111	2.15
DABN racemic mixture R03 exp93	2.21
BI-6727 0.04μM R03 exp98	2.34
2-Deoxy-D-glucose 800μM R05 exp214	2.5
Etoposide 1μM R00 exp19	2.5
Vinblastine 0.2μM R03 exp108	2.56
GSK461364A 0.1μM R03 exp126	2.58
Monensin 0.3μM R08 exp507	2.6
MLN-4924 2μM R00 exp22	3.24

Gene	Correlation
THG1L	0.718
DPAGT1	0.704
GFPT1	0.69
UAP1	0.658
RPTOR	0.648
GMPPB	0.645
DOHH	0.636
ALG1	0.632
GTF3C3	0.63
RNMT	0.628
TRIAP1	0.626
GNB1L	0.618
TRMT5	0.615
PMM2	0.614
RPP21	0.613
OSGEP	0.613
ATXN10	0.611
ADSL	0.609
NAE1	0.608
TSEN34	0.608
TTI2	0.608
DHPS	0.604
SRP14	0.602
EIF4E	0.601
POLR3H	0.593
YARS	0.592
EXOC4	0.589
CCDC101	0.589
MED16	0.588
GTF3C2	0.585
UHRF1	0.581
CCT7	0.572
HSPD1	0.569
GTF3C4	0.567
ALG14	0.566
AARS	0.566
ALG2	0.564
ALG13	0.564
PELO	0.561
GTF3C6	0.558
PPP4C	0.558
DDX1	0.552
VRK1	0.551
DDX20	0.551
TPRKB	0.545
PPCS	0.544
TELO2	0.54
CSNK2B	0.538
RPE	0.536
ENY2	0.534
NARS	0.532
TBCA	0.532
POLR3B	0.529
MEAF6	0.529
TTI1	0.529
HARS	0.527
MED4	0.526
CRCP	0.524
TSEN54	0.523
EPRS	0.52
RABGGTB	0.519
GEMIN8	0.519
VEZT	0.519
TGS1	0.518
TRNT1	0.517
DOLK	0.517
PLAA	0.516
CTC1	0.515
MLLT1	0.513
ZRSR2	0.513
ELP3	0.512
TEN1	0.512
ATP5J	0.512
DDX55	0.511
GEMIN5	0.51
ALG5	0.509
DPF2	0.508
RFT1	0.507
CMTR1	0.507
ORAOV1	0.506
TAZ	0.505
GEMIN6	0.503
UXT	0.502
UTP23	0.502
DR1	0.5
CEP57	0.5
LSM11	0.498
RPN1	0.496
RBM14	0.494
HAUS8	0.493
HCCS	0.491
FNTA	0.491
PAM16	0.491
RAD50	0.49
C15orf41	0.489
DDOST	0.488
DHX29	0.486
CBFB	0.485
TAMM41	0.485
WDR59	0.485
C14orf166	0.484
TMEM41B	0.484
UBE2G2	0.482
SSB	0.481
TBCB	0.48
SRP68	0.48
BAP1	0.479
SEH1L	0.479
NSMCE1	0.478
PPA2	0.478
CASP9	0.478
MARS	0.477
MTOR	0.476
UBA3	0.473
APAF1	0.473
WDR24	0.473
PYROXD1	0.471
DICER1	0.47
ATP5B	0.47
ENO1	0.47
LAMTOR5	0.47
VMP1	0.469
URM1	0.469
DHDDS	0.467
CTDNEP1	0.467
MAPK14	0.467
VHL	0.466
RAD1	0.466
DAD1	0.465
ELP2	0.465
BUB3	0.464
TIMM13	0.463
PAX5	0.463
SNAPC5	0.462
PPP6C	0.462
EXOC3	0.461
OTUD5	0.458
ZNF608	0.457
SLC25A26	0.457
FH	0.456
EEF1E1	0.455
UBE2K	0.455
PIK3R4	0.454
ELAC2	0.452
SRP72	0.451
SPCS2	0.448
TTC4	0.448
UBA5	0.446
DCTN6	0.445
OIP5	0.444
PGGT1B	0.444
PPP2R3C	0.444
GTF3C5	0.443
TSEN2	0.443
CARS	0.44
ATP5O	0.439
TRMT10A	0.439
TIMM22	0.439
SLC35B1	0.438
SUPV3L1	0.437
TXNL4B	0.436
TBP	0.436
EIF1AX	0.436
FAF2	0.434
WDR82	0.434
DCTN5	0.433
ALG6	0.433
NBN	0.432
THOC6	0.432
SEC62	0.431
DIABLO	0.43
ATIC	0.43
XRCC3	0.429
TP53RK	0.426
PDAP1	0.425
BAK1	0.424
RPIA	0.424
SNF8	0.423
ROMO1	0.423
NSD1	0.423
ZNF259	0.422
MAD2L1	0.422
EIF3F	0.421
NUP50	0.421
WDR92	0.42
TKT	0.419
NAA20	0.418
SRPRB	0.417
MED23	0.416
THOC3	0.416
LSMD1	0.415
SEC61G	0.415
NHLRC2	0.415
ACTR10	0.414
METAP2	0.414
TAF1A	0.413
SLC25A19	0.412
RFK	0.412
AASDHPPT	0.411
CHCHD4	0.411
TMED10	0.41
LAMTOR4	0.41
LSM10	0.41
GTF2A2	0.408
FBXW7	0.408
TCF4	0.408
ATP2A2	0.407
SSBP2	0.407
UBE2L3	0.407
TPK1	0.404
CINP	0.404
CRLS1	0.404
WDR1	0.403
ATM	0.403
SOD1	0.403
WARS	0.403
CIAO1	0.403
SRP19	0.402
SDHA	0.402
ATP5A1	0.402
CCDC115	0.402

Global Fraction of Cell Lines Where Essential: 422/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	14/28
blood	12/28
bone	13/26
breast	13/33
central nervous system	39/56
cervix	1/4
colorectal	9/17
esophagus	9/13
fibroblast	0/1
gastric	8/16
kidney	12/21
liver	12/20
lung	47/75
lymphocyte	6/16
ovary	15/26
pancreas	15/24
peripheral nervous system	8/16
plasma cell	5/15
prostate	0/1
skin	11/24
soft tissue	6/9
thyroid	2/2
upper aerodigestive	15/22
urinary tract	21/29
uterus	3/5

Essentiality in NALM6

Essentiality Rank: 925
Expression level (log2 read counts): 6.76

Expression Distribution

RNA ligase (ATP) activity
tRNA exon ligation
tRNA exon ligation utilizing 2,3 cyclic phosphate of 5-exon as source of linkage phosphate
RNA exon ligation
tRNA-splicing ligase complex
vinculin binding
tRNA splicing, via endonucleolytic cleavage and ligation
RNA splicing, via endonucleolytic cleavage and ligation
tRNA processing
placenta development
nuclear envelope
tRNA metabolic process
in utero embryonic development
ncRNA processing
RNA splicing
reproductive structure development
reproductive system development
ncRNA metabolic process
chordate embryonic development
embryo development ending in birth or egg hatching
developmental process involved in reproduction
intracellular membrane-bounded organelle
RNA processing
endoplasmic reticulum membrane
embryo development
RNA binding
reproductive process
reproduction
ATP binding
RNA metabolic process
gene expression