Chloramphenicol 2μM R00 exp12

Mechanism of Action

Binds 23S rRNA of 50S ribosomal subunit, inhibits protein synthesis

  • Class / Subclass 1: Infectious Disease / Antibiotic
  • Class / Subclass 2: Organelle Function / Mitochondrial Inhibitor

Technical Notes

Compound References

  • PubChem Name: Chloramphenicol
  • Synonyms: N/A
  • CAS #: 56-75-7
  • PubChem CID: 5959
  • IUPAC: 2,2-dichloro-N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]acetamide
  • INCHI Name: InChI=1S/C11H12Cl2N2O5/c12-10(13)11(18)14-8(5-16)9(17)6-1-3-7(4-2-6)15(19)20/h1-4,8-10,16-17H,5H2,(H,14,18)/t8-,9-/m1/s1
  • INCHI Key: WIIZWVCIJKGZOK-RKDXNWHRSA-N
  • Molecular Weight: 323.13
  • Canonical SMILES: C1=CC(=CC=C1C(C(CO)NC(=O)C(Cl)Cl)O)[N+](=O)[O-]
  • Isomeric SMILES: C1=CC(=CC=C1[C@H]([C@@H](CO)NC(=O)C(Cl)Cl)O)[N+](=O)[O-]
  • Molecular Formula: C11H12Cl2N2O5

Compound Supplier

  • Supplier Name: Sigma-Aldrich
  • Catalog #: C0378
  • Lot #: 096K1244

Compound Characterization

  • HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C11H12Cl2N2O5 323.0196; found 323.01899

Dose Response Curve

  • Platform ID: Chloramphenicol
  • Min: -5.6180; Max: 8.0817





IC Concentration (µM)
IC10 N/A
IC20 N/A
IC30 N/A
IC40 N/A
IC50 N/A
IC60 N/A
IC70 N/A
IC80 N/A
IC90 N/A


Screen Summary

  • Round: 00
  • Dose: 2µM
  • Days of incubation: 8
  • Doublings: 6.1
  • Numbers of reads: 12267396

Screen Results

Sensitive/Resistant hits (FDR<0.05)CRANKSScore PlotTop 30 GenesScreen SimilarityTop 30 Sensitive GO termsTop 30 Resistant GO terms
0/0ScoresViewViewViewViewView

  • Last modified: 2026/01/15 21:36
  • by 127.0.0.1