BI-6727 0.001μM R01 exp42

Mechanism of Action

Inhibits PLK1 kinase, causes G2/M arrest, improved version of BI-2536

  • Class / Subclass 1: Cell Cycle / Mitotic Inhibitor
  • Class / Subclass 2: Signal Transduction / Kinase Inhibitor

Technical Notes

Compound References

  • PubChem Name: Volasertib
  • Synonyms: BI 6727
  • CAS #: 755038-65-4
  • PubChem CID: 10461508
  • IUPAC: N-[4-[4-(cyclopropylmethyl)piperazin-1-yl]cyclohexyl]-4-[[(7R)-7-ethyl-5-methyl-6-oxo-8-propan-2-yl-7H-pteridin-2-yl]amino]-3-methoxybenzamide
  • INCHI Name: InChI=1S/C34H50N8O3/c1-6-28-33(44)39(4)29-20-35-34(38-31(29)42(28)22(2)3)37-27-14-9-24(19-30(27)45-5)32(43)36-25-10-12-26(13-11-25)41-17-15-40(16-18-41)21-23-7-8-23/h9,14,19-20,22-23,25-26,28H,6-8,10-13,15-18,21H2,1-5H3,(H,36,43)(H,35,37,38)/t25?,26?,28-/m1/s1
  • INCHI Key: SXNJFOWDRLKDSF-XKHVUIRMSA-N
  • Molecular Weight: 618.8
  • Canonical SMILES: CCC1C(=O)N(C2=CN=C(N=C2N1C(C)C)NC3=C(C=C(C=C3)C(=O)NC4CCC(CC4)N5CCN(CC5)CC6CC6)OC)C
  • Isomeric SMILES: CC[C@@H]1C(=O)N(C2=CN=C(N=C2N1C(C)C)NC3=C(C=C(C=C3)C(=O)NC4CCC(CC4)N5CCN(CC5)CC6CC6)OC)C
  • Molecular Formula: C34H50N8O3

Compound Supplier

  • Supplier Name: Med Chem Express
  • Catalog #: HY-12137
  • Lot #: N/A

Compound Characterization

  • HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C34H50N8O3 619.40786; found 619.4097

Dose Response Curve

  • Platform ID: BI6727
  • Min: -7.8407; Max: 99.1028





IC Concentration (µM)
IC10 N/A
IC20 0.0061
IC30 0.0129
IC40 0.0238
IC50 0.0420
IC60 N/A
IC70 N/A
IC80 N/A
IC90 N/A


Screen Summary

  • Round: 01
  • Dose: 1nM
  • Days of incubation: 8
  • Doublings: 6.8
  • Numbers of reads: 12280512

Screen Results

Sensitive/Resistant hits (FDR<0.05)CRANKSScore PlotTop 30 GenesScreen SimilarityTop 30 Sensitive GO termsTop 30 Resistant GO terms
0/0ScoresViewViewViewViewView

  • Last modified: 2026/01/15 21:36
  • by 127.0.0.1