APAF1

Gene Information

Official Symbol: APAF1
Official Name: apoptotic peptidase activating factor 1
Aliases and Previous Symbols: N/A
Entrez ID: 317
UniProt: O14727
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].
UniProt Summary: N/A

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
BML-284 0.09μM R08 exp460	1.7
ABT-702 5μM plus Deferoxamine 11μM R07 exp321	1.72
Ethanol 0.003 R08 exp437	1.72
Lead acetate 2000μM R08 exp497	1.73
ML-792 0.2μM R08 exp505	1.73
SNS-032 25μM R07 exp401	1.75
iCRT14 30μM R08 exp492	1.76
Monensin 0.3μM R08 exp507	1.78
Artemisinin 50μM R08 exp450	1.84
Dinaciclib 0.007μM R07 exp355	1.84
Temozolomide 200μM R04 exp189	1.84
UM0129480 7μM R02 exp85	1.87
Nocodazole 0.2μM R00 exp24	1.88
Rapamycin 10μM R00 exp30	1.89
LY2090314 0.003μM R08 exp500 no dilution day6	1.94
5-Azacytidine 2μM R07 exp330	1.98
6-Thio-2-deoxyguanosine 2μM R06 exp259	1.99
Diepoxybutane 3μM R07 exp354	1.99
GSK-J4 1-1.25μM to day4 R07 exp363	2.02
Thapsigargin 0.005μM R07 exp407	2.11
Rotenone 2μM R00 exp33	2.13
Menadione 5μM R06 exp292	2.39
Bleomycin 5μM R08 exp459	2.42
Rucaparib 6.5μM R08 exp520	2.45
CI-1040 9.5μM R08 exp472	2.47
Tozasertib 0.1μM R07 exp414	2.55
PU-H71 1μM R08 exp515	2.56
Centrinone 0.5μM R07 exp343	2.57
HMS-I1 10μM R06 exp256	2.64
Etoposide 0.1μM R05 exp198	2.66
DABN 20μM R00 exp17	2.73
Latrunculin-B 10μM R07 exp374	2.92
Ethanol 0.01 R05 exp234	2.96
Pyridostatin 4μM R05 exp240	2.98
Bortezomib 0.05μM R00 exp7	3.04
AICAR 240μM R03 exp116	3.15
Etoposide 10μM R00 exp20	3.16
Tanespimycin 14μM R08 exp527	3.23
Aphidicolin 0.4μM R08 exp440	3.25
MLN-4924 2μM R00 exp22	3.33
Dorsomorphin 5μM R07 exp357	3.47
AICAR 240μM R05 exp211	3.61
JQ1 0.8μM R08 exp435	3.65
RO-3307 4.7μM R02 exp80	3.7
MS023 2μM R03 exp134	3.74
GSK-J4 1.5μM R08 exp441	3.79
GSK626616 14μM R08 exp485	3.81
Hippuristanol 0.12μM R08 exp489 no dilution day6	3.84
THZ1 0.06μM R08 exp531	4.18
Doxorubicin 0.02μM R08 exp478	4.19
CR131-b 0.005μM R08 exp474	4.47
Benzoate 20000μM R08 exp456	4.55
SNS-032 15μM R08 exp443	4.67
Olaparib 4μM R08 exp512	4.97
Staurosporine 0.02μM R08 exp524	5.05
Hippuristanol 0.12μM R08 exp488	5.2
Mitomycin-C 0.06μM R08 exp503	5.39
Etoposide 1μM R00 exp19	5.65
Pimelic-diphenylamide-106 5μM R00 exp28	5.81
Etoposide 0.3μM R05 exp199	5.85
Rotenone 20μM R00 exp34	5.96
MS023 7μM R03 exp135	5.97
5-Fluorouracil 20μM R00 exp2	6.23
Actinomycin-D 0.001μM R00 exp3	6.28

Gene	Correlation
BAK1	0.82
CASP9	0.79
VDAC2	0.756
PLAA	0.756
MARCH5	0.744
CYCS	0.742
UBQLN1	0.726
HCCS	0.722
DIABLO	0.691
FAF2	0.653
HARS	0.635
DOHH	0.629
HSPD1	0.619
SERF2	0.602
ENY2	0.584
MFN1	0.576
ATAD1	0.575
BAP1	0.573
TRIAP1	0.572
ROMO1	0.561
CRLS1	0.56
THG1L	0.556
UAP1	0.554
RPTOR	0.547
STAU1	0.539
GABPA	0.536
GFPT1	0.535
CSNK2B	0.534
PPP2R3C	0.533
METAP2	0.517
MTOR	0.517
GMPPB	0.516
SRP14	0.51
PPA2	0.507
RAD23B	0.505
MEAF6	0.504
UBE2K	0.504
PMM2	0.501
TAZ	0.498
KDSR	0.497
DDHD2	0.493
VHL	0.487
ATP5J	0.486
SEC61G	0.481
TAMM41	0.479
PISD	0.476
RNMT	0.474
OPA1	0.473
RTCB	0.473
MLST8	0.471
UBE2G2	0.471
DPAGT1	0.47
NSD1	0.466
CTDNEP1	0.465
ATP2A2	0.463
UBA3	0.462
ATXN10	0.458
NAE1	0.456
ALG1	0.454
PIK3R4	0.454
GTF3A	0.45
ALG2	0.448
CBFB	0.448
FECH	0.447
ABCF1	0.447
RPN1	0.443
PAM16	0.442
TIMM13	0.441
NXT1	0.44
WDR59	0.439
EIF3F	0.439
WDR24	0.438
ATP5I	0.437
ZZZ3	0.436
PDPK1	0.433
ATP5O	0.431
ATP5A1	0.43
NUPL2	0.43
CEPT1	0.429
GTF3C6	0.427
BRWD1	0.427
TP53	0.427
DHPS	0.424
EXOC4	0.423
GTF3C3	0.423
SEC16A	0.419
ALG5	0.418
TRIM28	0.416
PPP5C	0.415
PELO	0.414
ATP5F1	0.413
CARS	0.412
SLC25A26	0.411
SLMO2	0.411
DAD1	0.411
TBCA	0.41
EIF4E	0.409
RBMX2	0.408
SRRD	0.407
ATP5B	0.407
UBE2M	0.406
GNAS	0.403
KCTD5	0.402
FBXO11	0.401

Global Fraction of Cell Lines Where Essential: 0/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 16386
Expression level (log2 read counts): 5.79

Expression Distribution

WD40
NB-ARC
CARD

response to G1 DNA damage checkpoint signaling
response to mitotic cell cycle checkpoint signaling
apoptosome
striated muscle cell apoptotic process
cardiac muscle cell apoptotic process
muscle cell apoptotic process
response to DNA damage checkpoint signaling
response to DNA integrity checkpoint signaling
response to cell cycle checkpoint signaling
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
regulation of apoptotic DNA fragmentation
regulation of DNA catabolic process
cysteine-type endopeptidase activator activity involved in apoptotic process
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
ADP binding
negative regulation of G0 to G1 transition
regulation of execution phase of apoptosis
regulation of G0 to G1 transition
neuron apoptotic process
neuron death
heat shock protein binding
nucleotide binding
activation of cysteine-type endopeptidase activity involved in apoptotic process
neural tube closure
tube closure
primary neural tube formation
neural tube formation
secretory granule lumen
embryonic epithelial tube formation
ficolin-1-rich granule lumen
epithelial tube formation
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
morphogenesis of embryonic epithelium
tube formation
intrinsic apoptotic signaling pathway
positive regulation of cysteine-type endopeptidase activity
cellular response to transforming growth factor beta stimulus
neural tube development
response to transforming growth factor beta
positive regulation of endopeptidase activity