Thapsigargin 0.005μM R07 exp407

Mechanism of Action

Inhibits sarco/endoplasmic reticulum Ca2+ ATPase (SERCA), non-competitive inhibitor, raises intracellular calcium concentration

  • Class / Subclass 1: Organelle Function / Membrane Transport Inhibitor
  • Class / Subclass 2: Signal Transduction / Ion Channel Inhibitor

Technical Notes

Compound References

  • PubChem Name: Thapsigargin
  • Synonyms: N/A
  • CAS #: 67526-95-8
  • PubChem CID: 446378
  • IUPAC: [(3S,3aR,4S,6S,6aR,7S,8S,9bS)-6-acetyloxy-4-butanoyloxy-3,3a-dihydroxy-3,6,9-trimethyl-8-[(Z)-2-methylbut-2-enoyl]oxy-2-oxo-4,5,6a,7,8,9b-hexahydroazuleno[4,5-b]furan-7-yl] octanoate
  • INCHI Name: InChI=1S/C34H50O12/c1-9-12-13-14-15-17-24(37)43-28-26-25(20(5)27(28)44-30(38)19(4)11-3)29-34(41,33(8,40)31(39)45-29)22(42-23(36)16-10-2)18-32(26,7)46-21(6)35/h11,22,26-29,40-41H,9-10,12-18H2,1-8H3/b19-11-/t22-,26+,27-,28-,29-,32-,33+,34+/m0/s1
  • INCHI Key: IXFPJGBNCFXKPI-FSIHEZPISA-N
  • Molecular Weight: 650.8
  • Canonical SMILES: CCCCCCCC(=O)OC1C2C(=C(C1OC(=O)C(=CC)C)C)C3C(C(CC2(C)OC(=O)C)OC(=O)CCC)(C(C(=O)O3)(C)O)O
  • Isomeric SMILES: CCCCCCCC(=O)O[C@H]1[C@H]2C(=C([C@@H]1OC(=O)/C(=C\\C)/C)C)[C@H]3[C@]([C@H](C[C@]2(C)OC(=O)C)OC(=O)CCC)([C@](C(=O)O3)(C)O)O
  • Molecular Formula: C34H50O12

Compound Supplier

  • Supplier Name: Cayman Chemical
  • Catalog #: 10522
  • Lot #: 0507161-12

Compound Characterization

  • HRMS (ESI-TOF) m/z: (M+Na)+ Calcd for C34H50O12 673.31945; found 673.32042

Dose Response Curve

  • Platform ID: Thapsigargin
  • Min: 14.5736; Max: 99.8187





IC Concentration (µM)
IC10 N/A
IC20 N/A
IC30 0.0032
IC40 0.0061
IC50 0.0103
IC60 N/A
IC70 N/A
IC80 N/A
IC90 N/A


Screen Summary

  • Round: 07
  • Dose: 5nM
  • Days of incubation: 8
  • Doublings: 3.4
  • Numbers of reads: 22303546

Screen Results

Sensitive/Resistant hits (FDR<0.05)CRANKSScore PlotTop 30 GenesScreen SimilarityTop 30 Sensitive GO termsTop 30 Resistant GO terms
34/54ScoresViewViewViewViewView

  • Last modified: 2026/01/15 21:36
  • by 127.0.0.1