Dexamethasone 0.006 to 0.015μM on day4 R07 exp352

Glucocorticoid receptor agonist, anti-inflammatory

Class / Subclass 1: Metabolism / Hormone Agonist
Class / Subclass 2: Signal Transduction / GPCR Agonist

Compound References

PubChem Name: Dexamethasone
Synonyms: Hexadecadrol; Prednisolone F
CAS #: 50-02-2
PubChem CID: 5743
IUPAC: (8S,9R,10S,11S,13S,14S,16R,17R)-9-fluoro-11,17-dihydroxy-17-(2-hydroxyacetyl)-10,13,16-trimethyl-6,7,8,11,12,14,15,16-octahydrocyclopenta[a]phenanthren-3-one
INCHI Name: InChI=1S/C22H29FO5/c1-12-8-16-15-5-4-13-9-14(25)6-7-19(13,2)21(15,23)17(26)10-20(16,3)22(12,28)18(27)11-24/h6-7,9,12,15-17,24,26,28H,4-5,8,10-11H2,1-3H3/t12-,15+,16+,17+,19+,20+,21+,22+/m1/s1
INCHI Key: UREBDLICKHMUKA-CXSFZGCWSA-N
Molecular Weight: 392.5
Canonical SMILES: CC1CC2C3CCC4=CC(=O)C=CC4(C3(C(CC2(C1(C(=O)CO)O)C)O)F)C
Isomeric SMILES: C[C@@H]1C[C@H]2[C@@H]3CCC4=CC(=O)C=C[C@@]4([C@]3([C@H](C[C@@]2([C@]1(C(=O)CO)O)C)O)F)C
Molecular Formula: C22H29FO5

Compound Supplier

Supplier Name: Sigma-Aldrich
Catalog #: D4902
Lot #: N/A

Compound Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C22H29FO5 393.20718; found 393.2068

Dose Response Curve

Platform ID: Dexamethasone
Min: -4.7517; Max: 71.3612

IC	Concentration (µM)
IC10	N/A
IC20	0.0111
IC30	0.0159
IC40	0.0223
IC50	0.0178
IC60	N/A
IC70	0.2312
IC80	N/A
IC90	N/A

Screen Summary

Round: 07
Dose: 0.006-0.015µM
Days of incubation: 8
Doublings: 5.2
Numbers of reads: 14131811

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
13/45	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
SPEN	-3.64	<0.01
FKBP5	-3.29	<0.01
NRAS	-3.23	<0.01
CREBBP	-3.07	<0.01
NFKBIA	-2.92	<0.01
CYLD	-2.86	<0.01
PPP2CA	-2.67	0.04
ERGIC1	-2.60	0.05
ZNF652	-2.57	0.05
DPH1	-2.54	0.04
DERL2	-2.52	0.04
SHOC2	-2.52	0.05
LEF1	-2.45	0.05
TRIM33	-2.43	0.05
MED16	-2.40	0.13
AFF4	-2.39	0.05
ABHD5	-2.36	0.10
SCAF8	-2.35	0.06
TCF4	-2.29	0.07
CTCF	-2.27	0.13
UBE4B	-2.24	0.10
TMSB4X	-2.22	0.14
DPF2	-2.21	0.10
PHIP	-2.20	0.10
RAP1GDS1	-2.17	0.12
GPR75-ASB3	-2.16	0.19
REC8	-2.14	0.20
LDB1	-2.14	0.13
UFSP2	-2.13	0.13
NSD1	-2.12	0.20
POLE2	2.59	0.14
SNRNP70	2.64	0.01
NF1	2.66	<0.01
TCF3	2.69	0.02
KPTN	2.69	<0.01
PPP3R1	2.70	<0.01
HIST1H4E	2.77	<0.01
KCTD5	2.77	<0.01
CCDC117	2.83	<0.01
UBE2H	2.89	<0.01
GPS2	2.98	<0.01
ARID3A	2.99	<0.01
EIF4B	3.10	0.00
SCAP	3.10	0.00
ZNF292	3.17	0.00
AXIN1	3.22	<0.01
NPRL3	3.34	<0.01
DDX3X	3.39	0.00
C12orf66	3.55	0.00
YPEL5	3.64	0.00
SUV39H1	3.75	0.00
GSK3B	3.82	0.00
NPRL2	3.87	0.00
NANS	4.11	0.00
ACTB	4.17	0.00
STIP1	4.54	0.00
PTGES3	4.73	0.00
TSC1	4.82	0.00
TSC2	7.07	0.00
NR3C1	7.72	0.00

Screen	Correlation	Plot
Dexamethasone 0.006μM R07 exp351	0.228	View
GSK626616 14μM R08 exp485	0.075	View
MK2206 4μM R08 exp504	0.074	View
Rapamycin 2μM plus Cyclosporin-A 3μM R07 exp307	0.064	View
Thapsigargin 0.005μM R07 exp407	0.063	View
I-BRD9 4μM R07 exp365	0.062	View
Rapamycin 2μM R07 exp306	0.061	View
Rapamycin 0.001μM R08 exp429	0.061	View
Rapamycin 1μM R00 exp29	0.06	View
Erlotinib 10μM R05 exp216	0.06	View
Momordin-Ic 10μM R08 exp506	0.054	View
Wortmannin 5μM R00 exp38	0.054	View
TRAIL 50ng/ml R00 exp36	0.052	View
CAY10603 0.55μM R08 exp467	0.052	View

GO Term	Fold Change	Genes
Seh1-associated complex	236.84	NPRL2,NPRL3,DEPDC5
negative regulation of TORC1 signaling	94.35	TSC2,TSC1,NPRL2,NPRL3,KPTN,DEPDC5,ITFG2
Hsp90 protein binding	69.08	NR3C1,TSC2,TSC1,PTGES3,STIP1
cellular response to amino acid starvation	58.79	NPRL2,NPRL3,KPTN,DEPDC5,ITFG2
negative regulation of TOR signaling	58.61	TSC2,TSC1,NPRL2,NPRL3,KPTN,DEPDC5,ITFG2
response to amino acid starvation	55.26	NPRL2,NPRL3,KPTN,DEPDC5,ITFG2
regulation of TORC1 signaling	44.98	TSC2,TSC1,NPRL2,NPRL3,KPTN,DEPDC5,ITFG2
regulation of TOR signaling	28.66	TSC2,TSC1,NPRL2,NPRL3,KPTN,DEPDC5,ITFG2
cellular response to starvation	26.01	TSC2,TSC1,NPRL2,SUV39H1,NPRL3,KPTN,DEPDC5,ITFG2
heat shock protein binding	22.28	NR3C1,TSC2,TSC1,PTGES3,STIP1
response to starvation	21.15	TSC2,TSC1,NPRL2,SUV39H1,NPRL3,KPTN,DEPDC5,ITFG2
cellular response to nutrient levels	19.06	TSC2,TSC1,NPRL2,SUV39H1,NPRL3,KPTN,DEPDC5,ITFG2
cellular response to extracellular stimulus	16.94	TSC2,TSC1,NPRL2,SUV39H1,NPRL3,KPTN,DEPDC5,ITFG2
cellular response to external stimulus	13.56	TSC2,TSC1,NPRL2,SUV39H1,NPRL3,KPTN,DEPDC5,ITFG2
negative regulation of intracellular signal transduction	11.85	TSC2,TSC1,NPRL2,GSK3B,DDX3X,NPRL3,GPS2,KPTN,NF1,DEPDC5,ITFG2
regulation of autophagy	11.12	TSC2,TSC1,NPRL2,GSK3B,NPRL3,SNRNP70,DEPDC5
response to nutrient levels	9.35	TSC2,TSC1,NPRL2,SUV39H1,NPRL3,KPTN,DEPDC5,ITFG2
response to extracellular stimulus	8.81	TSC2,TSC1,NPRL2,SUV39H1,NPRL3,KPTN,DEPDC5,ITFG2
intracellular protein-containing complex	6.95	TSC1,PTGES3,STIP1,ACTB,GSK3B,YPEL5,AXIN1,EIF4B,KCTD5,PPP3R1,POLE2

GO Term	Fold Change	Genes
beta-catenin-TCF complex	153.51	LEF1,TCF4,LDB1
transcription factor binding	8.33	SPEN,CREBBP,NFKBIA,LEF1,MED16,TCF4,CTCF,LDB1

Dexamethasone 0.006 to 0.015μM on day4 R07 exp352

Mechanism of Action

Technical Notes

Compound References

Compound Supplier

Compound Characterization

Dose Response Curve

Screen Summary

Screen Results