AXIN1

Gene Information

Official Symbol: AXIN1
Official Name: axin 1
Aliases and Previous Symbols: N/A
Entrez ID: 8312
UniProt: O15169
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016].
UniProt Summary: Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (PubMed:12192039, PubMed:27098453). Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway (PubMed:12192039). In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B (PubMed:12192039). Likely to function as a tumor suppressor. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (PubMed:16601693). Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development (PubMed:17210684). Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation (PubMed:17210684). {ECO:0000269|PubMed:12192039, ECO:0000269|PubMed:16601693, ECO:0000269|PubMed:17210684, ECO:0000269|PubMed:27098453}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
LY2090314 0.003μM R08 exp500 no dilution day6	-2.66
LY2090314 0.003μM R08 exp499	-2.5
6-Thio-2-deoxyguanosine 2μM R06 exp259	-1.93
All-trans-Retinoic-Acid 8μM R07 exp333	1.71
Dimethyloxaloylglycine 100μM R05 exp229	1.73
Geldanamycin 0.015 to 0.025μM on day4 R07 exp316	1.75
HMS-I1 1μM R01 exp46	1.81
Rapamycin 2μM plus FK-506 5μM R07 exp308	1.81
A-769662 20μM R07 exp331	1.87
Bafilomycin-A1 0.009μM R08 exp454	1.87
Betulinic acid 10 to 15μM on day4 R05 exp222	1.9
Calcium Ionophore 0.4μM R07 exp342	1.98
Demecolcine 0.03μM R05 exp228	2.01
2-Methoxyestradiol 0.55 to 1μM on day4 R07 exp312	2.02
Thapsigargin 0.005μM R07 exp407	2.04
Aminopterin 0.005μM R07 exp335	2.14
CyclicAMP 200μM R08 exp475	2.21
UMK57 1μM R01 exp59	2.31
2-Methoxyestradiol 0.55 to 0.75μM on day4 R07 exp311	2.33
All-trans-Retinoic-Acid 40μM R07 exp334	2.47
TRAIL 50ng/ml R00 exp36	2.5
Dexamethasone 0.006μM R07 exp351	2.89
Lead acetate 2000μM R08 exp498 no dilution day6	2.99
Lead acetate 2000μM R08 exp497	3
TRAIL 5ng/ml R00 exp35	3.09
Dexamethasone 0.006 to 0.015μM on day4 R07 exp352	3.22
I-BRD9 4μM R07 exp365	3.35

Global Fraction of Cell Lines Where Essential: 0/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	0/26
breast	0/33
central nervous system	0/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/16
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/16
ovary	0/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	0/24
soft tissue	0/9
thyroid	0/2
upper aerodigestive	0/22
urinary tract	0/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 1686
Expression level (log2 read counts): 5.17

Expression Distribution

Gene	Correlation
CTNNBIP1	0.422
SRRM2	0.421
SAMD4B	0.418

RGS
Axin b-cat bind
DIX

beta-catenin destruction complex assembly
armadillo repeat domain binding
I-SMAD binding
signaling adaptor activity
beta-catenin destruction complex
beta-catenin destruction complex disassembly
positive regulation of peptidyl-threonine phosphorylation
positive regulation of ubiquitin-protein transferase activity
cell periphery
regulation of intracellular estrogen receptor signaling pathway
regulation of peptidyl-threonine phosphorylation
molecular adaptor activity
regulation of ubiquitin-protein transferase activity
SMAD binding
lateral plasma membrane
regulation of intracellular steroid hormone receptor signaling pathway
beta-catenin binding
canonical Wnt signaling pathway
positive regulation of ubiquitin-dependent protein catabolic process
positive regulation of peptidyl-serine phosphorylation
positive regulation of protein ubiquitination
positive regulation of proteolysis involved in cellular protein catabolic process
positive regulation of protein modification by small protein conjugation or removal
regulation of peptidyl-serine phosphorylation
positive regulation of JNK cascade
positive regulation of cellular protein catabolic process
positive regulation of canonical Wnt signaling pathway
regulation of ubiquitin-dependent protein catabolic process
positive regulation of stress-activated MAPK cascade
positive regulation of stress-activated protein kinase signaling cascade
negative regulation of canonical Wnt signaling pathway
positive regulation of Wnt signaling pathway
regulation of JNK cascade
regulation of protein ubiquitination
negative regulation of Wnt signaling pathway
regulation of proteolysis involved in cellular protein catabolic process
positive regulation of protein catabolic process
regulation of protein modification by small protein conjugation or removal
regulation of stress-activated MAPK cascade
regulation of stress-activated protein kinase signaling cascade