TRAIL 5ng/ml R00 exp35

TNF-related apoptosis-inducing ligand, TNF superfamily member, induces extrinsic apoptosis

Class / Subclass 1: Signal Transduction / Other Receptor Agonist

Compound References

PubChem Name: N/A
Synonyms: N/A
CAS #: N/A
PubChem CID: N/A
IUPAC: N/A
INCHI Name: N/A
INCHI Key: N/A
Molecular Weight: 19.6kDa
Canonical SMILES: N/A
Isomeric SMILES: N/A
Molecular Formula: MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE

Compound Supplier

Supplier Name: Peprotech
Catalog #: 310-04
Lot #: N/A

Compound Characterization

Characterization data not available.

Dose Response Curve

Platform ID: Trail
Min: -9.0552; Max: 7.2598

IC	Concentration (µM)
IC10	N/A
IC20	N/A
IC30	N/A
IC40	N/A
IC50	N/A
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 00
Dose: 5ng/mL
Days of incubation: 8
Doublings: 2.9
Numbers of reads: 13057838

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
56/130	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
CNOT2	-4.63	<0.01
SZT2	-4.31	<0.01
ZBTB10	-4.23	<0.01
ARID4B	-3.86	<0.01
XIAP	-3.64	<0.01
C12orf66	-3.61	<0.01
PDS5B	-3.60	<0.01
C12orf44	-3.33	<0.01
CNOT8	-3.29	<0.01
TMEM30A	-3.28	<0.01
BCL11A	-3.28	<0.01
WDR20	-3.27	<0.01
ATG9A	-3.25	<0.01
WIPI2	-3.17	<0.01
OTUB1	-3.14	<0.01
PCBP2	-3.03	<0.01
RNF19A	-3.01	<0.01
ANKRD52	-3.00	<0.01
SESN2	-2.99	<0.01
TRAF2	-2.98	<0.01
MAPKAP1	-2.97	<0.01
RNF19B	-2.96	<0.01
DEPDC5	-2.85	<0.01
NPRL3	-2.82	0.01
ITFG2	-2.81	<0.01
ATG14	-2.79	<0.01
STUB1	-2.68	<0.01
SNRNP40	-2.64	<0.01
TSC2	-2.62	<0.01
MEF2D	-2.61	0.05
GCN1L1	4.27	<0.01
BTAF1	4.43	<0.01
IKZF1	4.47	<0.01
SEC31A	4.49	<0.01
EIF4A1	4.50	<0.01
CLK3	4.56	<0.01
CDK6	4.60	<0.01
TP53	4.63	<0.01
ZC3HAV1	4.65	<0.01
SPOP	4.65	<0.01
FNIP1	4.72	<0.01
FLCN	4.83	<0.01
CHD7	4.89	<0.01
DDX3X	4.91	<0.01
HNRNPL	5.27	<0.01
ZNF217	5.37	<0.01
MTMR4	5.59	<0.01
UBE3A	5.78	<0.01
CREB1	6.03	<0.01
CCNC	6.50	<0.01
KHDRBS1	6.50	<0.01
CXorf38	7.25	<0.01
IKZF2	7.29	<0.01
FBXO11	7.72	<0.01
TNFRSF10B	7.74	<0.01
ZBTB7A	7.85	<0.01
ELAVL1	8.00	<0.01
CASP3	8.14	<0.01
FADD	8.49	<0.01
CASP8	8.49	<0.01

Screen	Correlation	Plot
Tubacin 1.6μM R07 exp416	0.062	View
Thapsigargin 0.005μM R07 exp407	0.054	View
Wortmannin 5μM R00 exp38	0.051	View
TRAIL 50ng/ml R00 exp36	0.408	View
MLN-4924 0.2μM R00 exp21	0.087	View
3-Bromopyruvate 7μM R04 exp175	0.075	View
LY2090314 0.003μM R08 exp499	0.06	View
GSK626616 14μM R08 exp485	0.06	View
AICAR 240μM R05 exp211	0.06	View
Quercetin 20μM R08 exp517	0.058	View
Bortezomib 0.005μM R00 exp6	0.056	View
Aminopterin 0.005μM R07 exp335	0.056	View
Actinomycin-D 0.001μM R00 exp3	0.052	View
LY2090314 0.003μM R08 exp500 no dilution day6	0.052	View

GO Term	Fold Change	Genes
negative regulation of TORC1 signaling	97.06	SZT2,SESN2,DEPDC5,NPRL3,ITFG2,TSC2
cellular response to amino acid starvation	70.56	SZT2,SESN2,DEPDC5,NPRL3,ITFG2
response to amino acid starvation	66.32	SZT2,SESN2,DEPDC5,NPRL3,ITFG2
negative regulation of TOR signaling	60.29	SZT2,SESN2,DEPDC5,NPRL3,ITFG2,TSC2
cellular response to glucose starvation	55.27	SZT2,SESN2,ITFG2,ATG14
regulation of TORC1 signaling	53.98	SZT2,OTUB1,SESN2,DEPDC5,NPRL3,ITFG2,TSC2
cellular response to starvation	31.21	SZT2,WIPI2,SESN2,DEPDC5,NPRL3,ITFG2,ATG14,TSC2
regulation of TOR signaling	29.48	SZT2,SESN2,DEPDC5,NPRL3,ITFG2,TSC2
cellular response to nutrient levels	25.73	SZT2,WIPI2,SESN2,MAPKAP1,DEPDC5,NPRL3,ITFG2,ATG14,TSC2
response to starvation	25.39	SZT2,WIPI2,SESN2,DEPDC5,NPRL3,ITFG2,ATG14,TSC2
cellular response to extracellular stimulus	22.87	SZT2,WIPI2,SESN2,MAPKAP1,DEPDC5,NPRL3,ITFG2,ATG14,TSC2
cellular response to external stimulus	18.31	SZT2,WIPI2,SESN2,MAPKAP1,DEPDC5,NPRL3,ITFG2,ATG14,TSC2
vacuolar membrane	12.81	SZT2,TMEM30A,ATG9A,SESN2,DEPDC5,NPRL3,ITFG2,ATG14,TSC2
response to nutrient levels	12.62	SZT2,WIPI2,SESN2,MAPKAP1,DEPDC5,NPRL3,ITFG2,ATG14,TSC2
response to extracellular stimulus	11.89	SZT2,WIPI2,SESN2,MAPKAP1,DEPDC5,NPRL3,ITFG2,ATG14,TSC2
positive regulation of catabolic process	11.61	CNOT2,CNOT8,RNF19A,SESN2,RNF19B,DEPDC5,NPRL3,STUB1,TSC2
lytic vacuole membrane	10.95	SZT2,TMEM30A,SESN2,DEPDC5,NPRL3,ITFG2,TSC2
lysosomal membrane	10.95	SZT2,TMEM30A,SESN2,DEPDC5,NPRL3,ITFG2,TSC2
vacuole	8.82	SZT2,TMEM30A,ATG9A,WIPI2,SESN2,RNF19B,DEPDC5,NPRL3,ITFG2,ATG14,TSC2

GO Term	Fold Change	Genes
death effector domain binding	368.47	CASP8,FADD
TRAIL-activated apoptotic signaling pathway	276.35	CASP8,FADD,TNFRSF10B
death-inducing signaling complex	207.26	CASP8,FADD,CASP3
death receptor binding	82.91	CASP8,FADD,CASP3
extrinsic apoptotic signaling pathway via death domain receptors	52.64	CASP8,FADD,TNFRSF10B,DDX3X
regulation of TORC1 signaling	25.71	UBE3A,FLCN,FNIP1,LAMTOR2
intrinsic apoptotic signaling pathway	17.27	CASP3,TNFRSF10B,DDX3X,FLCN,TP53
lymphocyte differentiation	13.62	FADD,ZBTB7A,CHD7,FNIP1,TP53,CDK6,IKZF1
apoptotic signaling pathway	12.56	CASP8,FADD,CASP3,TNFRSF10B,DDX3X,FLCN,TP53
leukocyte differentiation	11.93	CASP8,FADD,ZBTB7A,CREB1,CHD7,FNIP1,TP53,CDK6,IKZF1
cellular response to external stimulus	11.87	CASP8,FADD,TNFRSF10B,FLCN,FNIP1,TP53,GCN1
mononuclear cell differentiation	11.83	FADD,ZBTB7A,CHD7,FNIP1,TP53,CDK6,IKZF1
mRNA binding	10.63	ELAVL1,KHDRBS1,HNRNPL,DDX3X,TP53,EIF4A1
cellular response to environmental stimulus	10.56	CASP8,FADD,CASP3,TNFRSF10B,DDX3X,TP53
lymphocyte activation	9.55	CASP8,FADD,ZBTB7A,CHD7,FNIP1,TP53,CDK6,IKZF1
hemopoiesis	9.11	CASP8,FADD,CASP3,ZBTB7A,CREB1,CHD7,FLCN,FNIP1,TP53,CDK6,IKZF1
positive regulation of apoptotic process	9.08	CASP8,FADD,CASP3,TNFRSF10B,DDX3X,FLCN,FNIP1,TP53
positive regulation of programmed cell death	8.70	CASP8,FADD,CASP3,TNFRSF10B,DDX3X,FLCN,FNIP1,TP53
leukocyte activation	7.58	CASP8,FADD,ZBTB7A,CHD7,FNIP1,TP53,CDK6,IKZF1

TRAIL 5ng/ml R00 exp35

Mechanism of Action

Technical Notes

Compound References

Compound Supplier

Compound Characterization

Dose Response Curve

Screen Summary

Screen Results