5-Azacytidine 2μM R07 exp330

Mechanism of Action

Analog of cytidine ribonucleoside, DNA methyltransferase inhibitor

  • Class / Subclass 1: Metabolism / Antimetabolite
  • Class / Subclass 2: Gene Regulation / Epigenetic Inhibitor
  • Class / Subclass 3: DNA Damage, Repair and Replication / Replication Inhibitor

Technical Notes

Compound References

  • PubChem Name: Azacitidine
  • Synonyms: Ladakamycin; 5-AzaC; Azacitidine
  • CAS #: 320-67-2
  • PubChem CID: 9444
  • IUPAC: 4-amino-1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-1,3,5-triazin-2-one
  • INCHI Name: InChI=1S/C8H12N4O5/c9-7-10-2-12(8(16)11-7)6-5(15)4(14)3(1-13)17-6/h2-6,13-15H,1H2,(H2,9,11,16)/t3-,4-,5-,6-/m1/s1
  • INCHI Key: NMUSYJAQQFHJEW-KVTDHHQDSA-N
  • Molecular Weight: 244.2
  • Canonical SMILES: C1=NC(=NC(=O)N1C2C(C(C(O2)CO)O)O)N
  • Isomeric SMILES: C1=NC(=NC(=O)N1[C@H]2[C@@H]([C@@H]([C@H](O2)CO)O)O)N
  • Molecular Formula: C8H12N4O5

Compound Supplier

  • Supplier Name: Toronto Research Chemicals
  • Catalog #: A796000
  • Lot #: 2-LXM-142-1

Compound Characterization

  • HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C8H12N4O5 245.08805; found 245.08809

Dose Response Curve

  • Platform ID: 5-Azacytidine
  • Min: -0.0848; Max: 97.5455





IC Concentration (µM)
IC10 N/A
IC20 0.8797
IC30 1.6236
IC40 2.6917
IC50 4.2961
IC60 N/A
IC70 N/A
IC80 N/A
IC90 N/A


Screen Summary

  • Round: 07
  • Dose: 2µM
  • Days of incubation: 8
  • Doublings: 4.1
  • Numbers of reads: 20253580

Screen Results

Sensitive/Resistant hits (FDR<0.05)CRANKSScore PlotTop 30 GenesScreen SimilarityTop 30 Sensitive GO termsTop 30 Resistant GO terms
2/7ScoresViewViewViewViewView

  • Last modified: 2026/01/15 21:36
  • by 127.0.0.1