Nocodazole 0.2μM R00 exp24

Inhibits microtubule polymerization and mitotic spindle formation, induces spindle assembly checkpoint (SAC)

Class / Subclass 1: Cell Cycle / Microtubule Poison
Class / Subclass 2: Organelle Function / Cytoskeletal Inhibitor

Compound References

PubChem Name: Nocodazole
Synonyms: Oncodazole; R17934
CAS #: 31430-18-9
PubChem CID: 4122
IUPAC: methyl N-[6-(thiophene-2-carbonyl)-1H-benzimidazol-2-yl]carbamate
INCHI Name: InChI=1S/C14H11N3O3S/c1-20-14(19)17-13-15-9-5-4-8(7-10(9)16-13)12(18)11-3-2-6-21-11/h2-7H,1H3,(H2,15,16,17,19)
INCHI Key: KYRVNWMVYQXFEU-UHFFFAOYSA-N
Molecular Weight: 301.32
Canonical SMILES: COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=CS3
Isomeric SMILES: N/A
Molecular Formula: C14H11N3O3S

Compound Supplier

Supplier Name: Sigma-Aldrich
Catalog #: M1404
Lot #: N/A

Compound Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C14H11N3O3S 302.05939; found 302.05966

Dose Response Curve

Platform ID: Nocodazole
Min: -9.8754; Max: 86.3481

IC	Concentration (µM)
IC10	N/A
IC20	0.0313
IC30	0.0403
IC40	0.0496
IC50	0.0601
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 00
Dose: 200nM
Days of incubation: 8
Doublings: 0.2
Numbers of reads: 11500839

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
0/139	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
POLA1	-3.81	0.10
GOLGA6L1	-3.34	0.10
ATP6V0C	-3.08	0.29
ATP6V1A	-2.97	0.05
INCENP	-2.91	0.05
ATP6V0B	-2.87	0.06
VPS28	-2.86	0.06
SYNCRIP	-2.74	0.09
TINF2	-2.58	0.10
TSC2	-2.58	0.06
XAB2	-2.55	0.25
DDX3X	-2.55	0.06
ATP6V0A2	-2.52	0.10
PSMB4	-2.42	0.16
ATP6V1D	-2.41	0.10
COPA	-2.37	0.33
BAG2	-2.37	0.10
SEPHS2	-2.34	0.10
TRAPPC11	-2.34	0.10
RPS11	-2.29	0.38
PCGF1	-2.29	0.10
LRRC16A	-2.27	0.10
VPS51	-2.26	0.16
NRTN	-2.26	0.10
EMC7	-2.25	0.16
SOX4	-2.21	0.25
NCAPD3	-2.21	0.12
AATF	-2.19	0.33
MTCH2	-2.19	0.13
AURKB	-2.18	0.40
HOXA3	3.28	<0.01
PLEKHA8	3.29	<0.01
IGF2BP3	3.30	<0.01
HOXA13	3.30	<0.01
CPVL	3.39	<0.01
WIPI2	3.41	<0.01
USP42	3.47	<0.01
CCZ1B	3.48	<0.01
TRIL	3.50	<0.01
EVX1	3.53	<0.01
CYTH3	3.58	<0.01
RNF216	3.61	<0.01
IGFBP1	3.63	<0.01
AMZ1	3.79	<0.01
CCZ1	3.83	<0.01
IQCE	3.84	<0.01
ZWILCH	4.08	<0.01
SDK1	4.25	<0.01
ADCYAP1R1	4.25	<0.01
MAD1L1	5.10	<0.01
MAD2L1BP	5.21	<0.01
MAD2L1	5.62	<0.01
BUB1B	5.81	<0.01
RBM14-RBM4	5.82	<0.01
CDC20	6.47	<0.01
RBM14	6.58	<0.01
BUB3	6.90	<0.01
TRIP13	7.81	<0.01
KNTC1	8.29	<0.01
ZW10	8.37	<0.01

Screen	Correlation	Plot
Rotenone 2μM R00 exp33	0.387	View
SB743921 0.001μM R05 exp244	0.311	View
Vinblastine 0.015μM R05 exp208	0.245	View
R-DABN 8μM R03 exp111	0.245	View
Nocodazole 0.2μM R02 exp64	0.212	View
Vinblastine 0.2μM R03 exp108	0.06	View
Pimelic-diphenylamide-106 5μM R00 exp28	0.053	View
Nocodazole 0.1μM R03 exp94	0.311	View
DABN racemic mixture R03 exp93	0.229	View
HMS-I1 10μM R06 exp256	0.206	View
DABN 20μM R00 exp17	0.111	View
BI-6727 0.04μM R03 exp98	0.085	View
Etoposide 1μM R00 exp19	0.063	View
Bortezomib 0.05μM R00 exp7	0.055	View

GO Term	Fold Change	Genes
Golgi lumen acidification	263.21	ATP6V0C,ATP6V1A,ATP6V0B,ATP6V1D
vacuolar proton-transporting V-type ATPase, V0 domain	197.40	ATP6V0C,ATP6V0B,ATP6V0A2
ATPase-coupled ion transmembrane transporter activity	128.74	ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D
proton-transporting ATPase activity, rotational mechanism	128.74	ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D
ATPase activity, coupled to transmembrane movement of ions, rotational mechanism	128.74	ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D
vacuolar proton-transporting V-type ATPase complex	123.38	ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D
lysosomal lumen acidification	112.80	ATP6V0C,ATP6V1A,ATP6V0B,ATP6V1D
proton-transporting V-type ATPase complex	109.67	ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D
regulation of lysosomal lumen pH	84.60	ATP6V0C,ATP6V1A,ATP6V0B,ATP6V1D
vacuolar acidification	71.78	ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D
proton-transporting two-sector ATPase complex	65.80	ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D
intracellular pH reduction	58.06	ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D
ATPase-coupled monoatomic cation transmembrane transporter activity	58.06	ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D
regulation of intracellular pH	34.04	ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D
endosome organization	33.65	ATP6V0C,ATP6V1A,ATP6V0B,VPS28,ATP6V1D
regulation of cellular pH	32.19	ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D
ATPase-coupled transmembrane transporter activity	31.17	ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D
regulation of pH	29.03	ATP6V0C,ATP6V1A,ATP6V0B,ATP6V0A2,ATP6V1D
regulation of macroautophagy	23.07	ATP6V0C,ATP6V1A,ATP6V0B,TSC2,ATP6V0A2,ATP6V1D

GO Term	Fold Change	Genes
mitotic spindle checkpoint signaling	177.66	ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH
spindle assembly checkpoint signaling	177.66	ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH
mitotic spindle assembly checkpoint signaling	177.66	ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH
negative regulation of mitotic sister chromatid separation	160.47	ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH
negative regulation of mitotic sister chromatid segregation	160.47	ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH
spindle checkpoint signaling	152.48	ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD1L1,ZWILCH
negative regulation of chromosome separation	150.75	ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH
negative regulation of metaphase/anaphase transition of cell cycle	150.75	ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH
negative regulation of chromosome segregation	150.75	ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH
negative regulation of mitotic metaphase/anaphase transition	142.64	ZW10,KNTC1,TRIP13,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH
negative regulation of sister chromatid segregation	142.64	ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD1L1,ZWILCH
negative regulation of nuclear division	108.14	ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD1L1,ZWILCH
regulation of mitotic sister chromatid segregation	93.86	ZW10,KNTC1,TRIP13,BUB3,BUB1B,MAD2L1,MAD2L1BP,MAD1L1,ZWILCH
regulation of mitotic sister chromatid separation	85.77	ZW10,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD2L1BP,MAD1L1,ZWILCH
regulation of chromosome separation	75.72	ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD2L1BP,MAD1L1,ZWILCH
regulation of mitotic metaphase/anaphase transition	62.10	ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD2L1BP,MAD1L1,ZWILCH
regulation of metaphase/anaphase transition of cell cycle	60.08	ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD2L1BP,MAD1L1,ZWILCH
regulation of mitotic nuclear division	51.97	ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD2L1BP,MAD1L1,ZWILCH,HOXA13
regulation of nuclear division	42.22	ZW10,KNTC1,TRIP13,BUB3,CDC20,BUB1B,MAD2L1,MAD2L1BP,MAD1L1,ZWILCH,HOXA13

Nocodazole 0.2μM R00 exp24

Mechanism of Action

Technical Notes

Compound References

Compound Supplier

Compound Characterization

Dose Response Curve

Screen Summary

Screen Results