UBE2L3

Gene Information

Official Symbol: UBE2L3
Official Name: ubiquitin conjugating enzyme E2 L3
Aliases and Previous Symbols: N/A
Entrez ID: 7332
UniProt: P68036
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down- regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis. {ECO:0000269|PubMed:10888878, ECO:0000269|PubMed:15367689, ECO:0000269|PubMed:17003263, ECO:0000269|PubMed:18946090, ECO:0000269|PubMed:19340006, ECO:0000269|PubMed:20061386, ECO:0000269|PubMed:21532592}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Etoposide 0.1μM R05 exp198	-2.16
Dimethyl-Sulfoxide 1pc R03 exp120	-2.1
Methotrexate 0.01μM R08 exp501	-2.08
Azithromycin 100μM R08 exp452	-2.02
Ibrutinib 10μM R08 exp442	-1.86
Pyronaridine 1μM R06 exp295	-1.73
5-Fluorouracil 20μM R00 exp2	2.06
UMK57 1μM R01 exp59	2.64
UM131593 0.2μM R03 exp106	2.65

Gene	Correlation
CCDC101	0.534
OTUD5	0.533
NAE1	0.493
UBR5	0.489
TLE4	0.474
VCPIP1	0.466
THOC6	0.466
MED25	0.461
ARIH1	0.457
AARS	0.448
HHEX	0.446
ID3	0.444
NXT1	0.44
MLLT1	0.439
IRF2BP2	0.433
XPO6	0.433
UBA3	0.432
MEAF6	0.429
TRMT5	0.427
DDX5	0.426
MAPK14	0.425
FLI1	0.423
CBX3	0.419
TADA1	0.416
RNMT	0.415
BAP1	0.412
TADA3	0.412
CARS	0.409
DOHH	0.408
NSD1	0.407
RTCB	0.407
TTI2	0.404
BRPF1	0.404
MED23	0.404
GLMN	0.404
XPOT	0.402

Global Fraction of Cell Lines Where Essential: 157/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	7/28
blood	1/28
bone	12/25
breast	9/33
central nervous system	12/56
cervix	0/4
colorectal	4/17
esophagus	3/13
fibroblast	0/1
gastric	2/15
kidney	7/21
liver	4/20
lung	16/75
lymphocyte	1/14
ovary	3/26
pancreas	7/24
peripheral nervous system	4/16
plasma cell	2/15
prostate	0/1
skin	1/24
soft tissue	3/7
thyroid	0/2
upper aerodigestive	4/22
urinary tract	8/29
uterus	3/5

Essentiality in NALM6

Essentiality Rank: 2058
Expression level (log2 read counts): 6.46

Expression Distribution

ubiquitin-protein transferase activator activity
protein K11-linked ubiquitination
positive regulation of ubiquitin-protein transferase activity
positive regulation of protein targeting to mitochondrion
ubiquitin conjugating enzyme activity
regulation of protein targeting to mitochondrion
regulation of ubiquitin-protein transferase activity
cellular response to glucocorticoid stimulus
positive regulation of establishment of protein localization to mitochondrion
cellular response to corticosteroid stimulus
regulation of establishment of protein localization to mitochondrion
regulation of protein targeting
ubiquitin ligase complex
positive regulation of mitochondrion organization
positive regulation of protein ubiquitination
positive regulation of protein modification by small protein conjugation or removal
response to glucocorticoid
positive regulation of intracellular protein transport
response to corticosteroid
regulation of mitochondrion organization
cellular response to steroid hormone stimulus
regulation of protein ubiquitination
positive regulation of intracellular transport
regulation of protein modification by small protein conjugation or removal
regulation of intracellular protein transport
ubiquitin-protein transferase activity
cell cycle phase transition
transcription coactivator activity
ubiquitin protein ligase binding
protein polyubiquitination
positive regulation of cellular protein localization
response to steroid hormone
enzyme binding
regulation of intracellular transport
positive regulation of protein transport
positive regulation of establishment of protein localization
cellular response to lipid
ubiquitin-dependent protein catabolic process
modification-dependent protein catabolic process
regulation of cellular protein localization