MED25

Gene Information

Official Symbol: MED25
Official Name: mediator complex subunit 25
Aliases and Previous Symbols: N/A
Entrez ID: 81857
UniProt: Q71SY5
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq, Apr 2010].
UniProt Summary: N/A

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Lead acetate 2000μM R08 exp497	-2.64
Cadmium 60μM R08 exp462	-2.43
Hypoxia 5%O2 R04 exp191	-2.01
CCCP 1μM R00 exp11	-1.94
Trichostatin-A 0.06μM R07 exp415	-1.82
Ribavirin 10 to 15μM on day4 R04 exp160	-1.81
UM131593 0.2μM R03 exp106	1.71
SNS-032 15μM R08 exp443	2
Senexin-A 25μM R07 exp400	2.01
BML-284 0.09μM R08 exp460	2.1
CI-1040 9.5μM R08 exp472	2.17
Palbociclib 1μM R07 exp382	2.22

Gene	Correlation
CCDC101	0.517
MLLT1	0.49
DPAGT1	0.465
TLE4	0.464
THOC6	0.461
UBE2L3	0.461
MED16	0.455
ALG5	0.45
SUPT7L	0.445
OTUD5	0.438
IRF2BP2	0.437
TRIM28	0.434
NAE1	0.433
GFPT1	0.431
HARS	0.423
FH	0.421
DNAJC3	0.414
TAF5L	0.414
ATP5J	0.413
LSM10	0.411
ATXN10	0.41
TRMT5	0.409
BTAF1	0.408
GNB1L	0.407
ID3	0.407
UBR5	0.407
CBX3	0.406
MED13L	0.404
TAF6L	0.402
TSEN54	0.402
FBXO11	0.401
BAP1	0.401
VMP1	0.4
DOLK	0.4

Global Fraction of Cell Lines Where Essential: 6/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	0/1
bile duct	0/28
blood	0/28
bone	1/25
breast	0/33
central nervous system	1/56
cervix	0/4
colorectal	0/17
esophagus	0/13
fibroblast	0/1
gastric	0/15
kidney	0/21
liver	0/20
lung	0/75
lymphocyte	0/14
ovary	1/26
pancreas	0/24
peripheral nervous system	0/16
plasma cell	0/15
prostate	0/1
skin	1/24
soft tissue	0/7
thyroid	0/2
upper aerodigestive	0/22
urinary tract	2/29
uterus	0/5

Essentiality in NALM6

Essentiality Rank: 2698
Expression level (log2 read counts): 5.05

Expression Distribution

Med25 VWA
Med25 SD1
Med25
Med25 NR-box

regulation of mediator complex assembly
positive regulation of mediator complex assembly
retinoic acid receptor binding
positive regulation of chromatin binding
retinoid X receptor binding
regulation of chromatin binding
nuclear transcription factor complex
negative regulation of fibroblast proliferation
mediator complex
positive regulation of cell cycle arrest
regulation of fibroblast proliferation
regulation of cell cycle arrest
transcription initiation from RNA polymerase II promoter
positive regulation of binding
DNA-templated transcription, initiation
positive regulation of protein complex assembly
positive regulation of cell cycle process
transcription factor binding
regulation of binding
positive regulation of cell cycle
regulation of protein complex assembly
transcription by RNA polymerase II
positive regulation of cellular component biogenesis
transcription, DNA-templated
nucleic acid-templated transcription
RNA biosynthetic process
negative regulation of cell population proliferation
regulation of cell cycle process
negative regulation of transcription by RNA polymerase II
regulation of cellular component biogenesis
nucleobase-containing compound biosynthetic process
heterocycle biosynthetic process
aromatic compound biosynthetic process
regulation of cell cycle
negative regulation of transcription, DNA-templated
positive regulation of cellular component organization
positive regulation of transcription by RNA polymerase II
negative regulation of nucleic acid-templated transcription
negative regulation of RNA biosynthetic process
organic cyclic compound biosynthetic process