BI-2536 0.02μM R03 exp96

Inhibits PLK1 kinase, causes G2/M arrest

PubChem Name: BI-2536
Synonyms: N/A
CAS #: 755038-02-9
PubChem CID: 11364421
IUPAC: 4-[[(7R)-8-cyclopentyl-7-ethyl-5-methyl-6-oxo-7H-pteridin-2-yl]amino]-3-methoxy-N-(1-methylpiperidin-4-yl)benzamide
INCHI Name: InChI=1S/C28H39N7O3/c1-5-22-27(37)34(3)23-17-29-28(32-25(23)35(22)20-8-6-7-9-20)31-21-11-10-18(16-24(21)38-4)26(36)30-19-12-14-33(2)15-13-19/h10-11,16-17,19-20,22H,5-9,12-15H2,1-4H3,(H,30,36)(H,29,31,32)/t22-/m1/s1
INCHI Key: XQVVPGYIWAGRNI-JOCHJYFZSA-N
Molecular Weight: 521.7
Canonical SMILES: CCC1C(=O)N(C2=CN=C(N=C2N1C3CCCC3)NC4=C(C=C(C=C4)C(=O)NC5CCN(CC5)C)OC)C
Isomeric SMILES: CC[C@@H]1C(=O)N(C2=CN=C(N=C2N1C3CCCC3)NC4=C(C=C(C=C4)C(=O)NC5CCN(CC5)C)OC)C
Molecular Formula: C28H39N7O3

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
0/20	Scores	View	View	View	View	View

Screen	Correlation	Plot
BI-6727 0.04μM R03 exp98	0.2	View
GSK461364A 0.1μM R03 exp126	0.175	View
Vinblastine 0.2μM R03 exp108	0.119	View
SNS-032 15μM R08 exp443	0.096	View
Hinokiflavone 12μM R08 exp487	0.086	View
DABN racemic mixture R03 exp93	0.082	View
Mitomycin-C 0.06μM R08 exp503	0.078	View
THZ1 0.06μM R08 exp531	0.078	View
Doxorubicin 0.02μM R08 exp478	0.075	View
R-DABN 8μM R03 exp111	0.071	View
MLN-4924 2μM R00 exp22	0.066	View
Etoposide 0.3μM R05 exp199	0.066	View
Nocodazole 0.1μM R03 exp94	0.064	View
Bleomycin 5μM R08 exp459	0.063	View
MS023 7μM R03 exp135	0.057	View
HMS-I1 10μM R06 exp256	0.055	View
Nocodazole 0.2μM R02 exp64	0.054	View
CI-1040 9.5μM R08 exp472	0.053	View
DABN 20μM R00 exp17	0.053	View
UM131593 0.2μM R03 exp106	0.052	View
Atovaquone 15μM R08 exp451	0.074	View
Ammonium tetrathiomolybdate 10μM R08 exp448	0.071	View
42°C R08 exp539	0.061	View
5-Fluorouracil 20μM R00 exp2	0.051	View
6-Thio-2-deoxyguanosine 2μM R06 exp259	0.051	View

GO Term	Fold Change	Genes
ESCRT complex	70.85	CHMP2A,VPS25,CHMP4B
multivesicular body assembly	61.40	CHMP2A,VPS25,CHMP4B
multivesicular body organization	59.42	CHMP2A,VPS25,CHMP4B
late endosome to vacuole transport	54.18	CHMP2A,VPS25,CHMP4B
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	52.63	CHMP2A,VPS25,CHMP4B
endosome transport via multivesicular body sorting pathway	47.23	CHMP2A,VPS25,CHMP4B
membrane fission	44.93	CHMP2A,VPS25,CHMP4B
proteasome complex	40.94	PSMA5,PSMA1,PSMD3,PSMA7
modification-dependent macromolecule catabolic process	9.23	PSMA5,PSMA1,CHMP2A,TUT4,VPS25,PSMD3,CHMP4B,PSMA7
ubiquitin-dependent protein catabolic process	8.41	PSMA5,PSMA1,CHMP2A,VPS25,PSMD3,CHMP4B,PSMA7
modification-dependent protein catabolic process	8.25	PSMA5,PSMA1,CHMP2A,VPS25,PSMD3,CHMP4B,PSMA7
proteolysis involved in protein catabolic process	7.37	PSMA5,PSMA1,CHMP2A,VPS25,PSMD3,CHMP4B,PSMA7
macromolecule catabolic process	6.66	PSMA5,PSMA1,CSDE1,CHMP2A,TUT4,SMG7,VPS25,PSMD3,CHMP4B,PSMA7

GO Term	Fold Change	Genes
mitotic checkpoint complex	368.42	BUB3,MAD2L1
RNA polymerase III complex	130.03	POLR3B,POLR3H,POLR3C,CRCP
DNA-directed 5-3 RNA polymerase activity	85.02	POLR3B,POLR3H,POLR3C,CRCP
5-3 RNA polymerase activity	78.95	POLR3B,POLR3H,POLR3C,CRCP
RNA polymerase activity	78.95	POLR3B,POLR3H,POLR3C,CRCP
mitotic spindle assembly checkpoint signaling	59.21	BUB3,MAD2L1,MAD1L1
mitotic spindle checkpoint signaling	59.21	BUB3,MAD2L1,MAD1L1
spindle assembly checkpoint signaling	59.21	BUB3,MAD2L1,MAD1L1
transcription by RNA polymerase III	58.17	POLR3B,POLR3H,CRCP,GTF3A
negative regulation of mitotic sister chromatid segregation	53.48	BUB3,MAD2L1,MAD1L1
negative regulation of mitotic sister chromatid separation	53.48	BUB3,MAD2L1,MAD1L1
negative regulation of metaphase/anaphase transition of cell cycle	50.24	BUB3,MAD2L1,MAD1L1
negative regulation of chromosome segregation	50.24	BUB3,MAD2L1,MAD1L1
nuclear DNA-directed RNA polymerase complex	24.03	POLR3B,POLR3H,POLR3C,CRCP
DNA-directed RNA polymerase complex	23.27	POLR3B,POLR3H,POLR3C,CRCP
RNA polymerase complex	22.79	POLR3B,POLR3H,POLR3C,CRCP
nucleotidyltransferase activity	20.32	POLR3B,THG1L,POLR3H,POLR3C,CRCP
catalytic activity, acting on RNA	11.51	POLR3B,THG1L,RNMT,POLR3H,RPP21,POLR3C,CRCP,TRMT61A
catalytic activity, acting on a nucleic acid	7.42	POLR3B,THG1L,RNMT,POLR3H,RPP21,POLR3C,CRCP,TRMT61A