RO-3306 3 to 4μM on day4 R04 exp165 [The Human Chemical-Genetic Interaction Map Project]

Inhibits CDK1, ATP-competitive, causes G2/M arrest

Class / Subclass 1: Cell Cycle / Kinase Inhibitor

Compound References

PubChem Name: (5Z)-5-(Quinolin-6-ylmethylidene)-2-[(thiophen-2-ylmethyl)amino]-1,3-thiazol-4(5H)-one
Synonyms: N/A
CAS #: 872573-93-8
PubChem CID: 135400873
IUPAC: (5Z)-5-(quinolin-6-ylmethylidene)-2-(thiophen-2-ylmethylimino)-1,3-thiazolidin-4-one
INCHI Name: InChI=1S/C18H13N3OS2/c22-17-16(24-18(21-17)20-11-14-4-2-8-23-14)10-12-5-6-15-13(9-12)3-1-7-19-15/h1-10H,11H2,(H,20,21,22)/b16-10-
INCHI Key: XOLMRFUGOINFDQ-YBEGLDIGSA-N
Molecular Weight: 351.4
Canonical SMILES: C1=CC2=C(C=CC(=C2)C=C3C(=O)NC(=NCC4=CC=CS4)S3)N=C1
Isomeric SMILES: C1=CC2=C(C=CC(=C2)/C=C\\3/C(=O)NC(=NCC4=CC=CS4)S3)N=C1
Molecular Formula: C18H13N3OS2

Compound Supplier

Supplier Name: Tocris Bioscience
Catalog #: 4181
Lot #: N/A

Compound Characterization

HRMS (ESI-TOF) m/z: (M+H)+ Calcd for C18H13N3OS2 352.05728; found 352.05802

Dose Response Curve

Platform ID: RO-3306
Min: -19.9269; Max: 63.7506

IC	Concentration (µM)
IC10	N/A
IC20	5.6881
IC30	7.8448
IC40	10.2102
IC50	13.0035
IC60	N/A
IC70	N/A
IC80	N/A
IC90	N/A

Screen Summary

Round: 04
Dose: 3-4µM
Days of incubation: 8
Doublings: 7.1
Numbers of reads: 18779480

Sensitive/Resistant hits (FDR<0.05)	CRANKS	Score Plot	Top 30 Genes	Screen Similarity	Top 30 Sensitive GO terms	Top 30 Resistant GO terms
0/0	Scores	View	View	View	View	View

Gene	CRANKS Score	FDR
QRICH1	-2.80	0.20
KDSR	-2.59	0.20
C17orf85	-2.44	0.22
CEP57	-2.43	0.22
ZNF519	-2.42	0.20
MTSS1L	-2.34	0.22
CCNB1	-2.26	0.36
LAMTOR4	-2.26	0.22
FKBP14	-2.17	0.32
NPRL2	-2.07	0.46
ALKBH1	-2.07	0.59
TNFRSF10D	-2.03	0.53
ATP2B1	-2.03	0.59
RSL24D1	-2.02	0.59
KDM6B	-1.98	0.59
RBMS1	-1.96	0.59
TNFRSF1A	-1.95	0.59
DRD5	-1.91	0.59
CENPN	-1.90	0.59
RMI1	-1.89	0.59
RTF1	-1.87	0.59
PGGT1B	-1.86	0.59
TAF5L	-1.86	0.59
HSPA1L	-1.85	0.59
CCDC101	-1.85	0.59
DCTN5	-1.85	0.59
LPP	-1.85	0.59
CADPS	-1.83	0.59
C1orf131	-1.83	0.63
MYADM	-1.82	0.59
HK1	1.98	0.48
FAM231A	2.00	0.50
FAM231C	2.00	0.50
CDKN1A	2.00	0.48
ANKRD7	2.03	0.44
RFC2	2.06	0.60
MMS22L	2.07	0.50
PAFAH1B1	2.10	0.71
C1orf198	2.10	0.34
CCDC135	2.12	0.33
MANSC4	2.12	0.33
CRNKL1	2.13	0.50
GPR183	2.14	0.33
PPP1R13L	2.14	0.33
TBC1D3I	2.15	0.68
TBC1D3E	2.16	0.68
TBC1D3K	2.16	0.68
TBC1D3L	2.16	0.68
HAP1	2.17	0.43
HSF1	2.20	0.33
CDC45	2.26	0.52
RSL1D1	2.32	0.33
KAT8	2.32	0.33
KARS	2.38	0.50
SMC4	2.45	0.33
TRAIP	2.49	0.33
FAM189A2	2.51	0.33
RPS6	2.54	0.50
RPL31	2.55	0.33
SMG5	2.59	0.60

Last modified: 2026/01/15 21:36
by 127.0.0.1