CDC45

Gene Information

Official Symbol: CDC45
Official Name: cell division cycle 45
Aliases and Previous Symbols: N/A
Entrez ID: 8318
UniProt: O75419
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013].
UniProt Summary: Required for initiation of chromosomal DNA replication.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
BI-D1870 3.15μM R02 exp66	-2.43
Palbociclib 1μM R07 exp382	-2.09
Thimerosal 0.85μM R08 exp529	-2.06
Torin1 0.08μM R02 exp82	-1.96
Rotenone 2μM R00 exp33	-1.85
Monensin 0.3μM R08 exp507	-1.7
L-BMAA 500 to 750μM on day4 R04 exp185	1.7
Nifuroxazide 1μM R01 exp51	1.73
UM0011500 5μM R05 exp245	1.74
NFN1 0.1μM R01 exp49	1.76
Cycloheximide 0.02μM R00 exp14	1.78
Mubritinib 0.2μM R01 exp48	1.78
All-trans-Retinoic-Acid 40μM R07 exp334	1.79
Cidofovir 10μM R07 exp345	1.82
NN-Diethyl-meta-toluamide 500μM R08 exp438	1.83
BAY-598 4μM R05 exp220	1.88
HMS-I2 10μM R06 exp288	1.89
Celastrol 0.12μM R05 exp225	1.9
Zebularine 20μM R07 exp423	1.91
Rapamycin 2μM plus FK-506 5μM R07 exp308	1.95
Alpha-Amanitin 0.5μM R00 exp5	1.97
MK-1775 0.32μM R02 exp75	1.99
Apcin 25μM plus proTAME 2μM R04 exp177	2.01
SGC0946 7μM R03 exp151	2.04
Hypoxia 5%O2 R04 exp191	2.06
3-Bromopyruvate 7μM R04 exp175	2.09
A-395 10μM R05 exp218	2.12
Cadmium 60μM R08 exp462	2.12
Dynasore 7μM R08 exp436	2.18
Menadione 5μM R06 exp292	2.18
Senexin-A 25μM R07 exp400	2.18
GSK461364A 0.005μM R03 exp125	2.2
RO-3306 3 to 4μM on day4 R04 exp165	2.26
Vincristine 0.0005μM R04 exp190	2.27
Dihydrosphingosine 8μM R08 exp476	2.34
FTY720 3μM R08 exp483	2.36
Rapamycin 2μM plus Cyclosporin-A 3μM R07 exp307	2.42
Rifampicin 1μM R00 exp31	2.42
CHIR-124 0.04μM R06 exp272	2.58
Metformin 100 to 150μM on day4 R04 exp170	2.85

Gene	Correlation
PSMB4	0.571
PSMA6	0.559
TWISTNB	0.543
CBWD5	0.543
CCNA2	0.541
TSPY8	0.528
CBWD3	0.526
POLR2J3	0.522
UPF1	0.52
TBC1D3H	0.518
TFIP11	0.516
PSMD1	0.514
UTP20	0.514
ACSF2	0.511
SLC4A1	0.506
C1QTNF9B	0.503
TCEB3CL2	0.5
ZNF830	0.494
RRP7A	0.492
CHMP4B	0.492
TCEB3C	0.489
SYT9	0.487
CBWD6	0.487
RAD21	0.487
TBC1D3F	0.486
NUPL1	0.486
WDR43	0.482
TCEB3CL	0.482
IK	0.482
RPS12	0.479
TBC1D3G	0.479
POLE	0.477
NUP160	0.477
SUPT5H	0.475
RRP15	0.474
WDR75	0.473
HAP1	0.471
LRR1	0.471
PSMB3	0.471
SSH2	0.47
PSMA1	0.47
CCDC116	0.469
PSMD3	0.468
KCTD17	0.467
DOCK6	0.466
AATF	0.465
PWP2	0.464
VASN	0.462
TBC1D3C	0.462
PSMC2	0.462
POLA2	0.462
MT1E	0.462
CHCHD3	0.461
RPS2	0.461
PSMB2	0.461
RRN3	0.458
EXOSC5	0.457
MED17	0.457
ZFAND5	0.456
COPE	0.454
DDTL	0.454
SEC13	0.454
RAD51	0.453
OR10G3	0.453
ATP6V1C1	0.452
SUPT6H	0.451
OGFR	0.451
TRAIP	0.45
MYO1H	0.448
PSMA7	0.447
ISCU	0.445
KRT78	0.445
FAM25B	0.445
RPS19BP1	0.444
NBPF10	0.443
COL2A1	0.442
RPL35	0.442
NBPF16	0.441
TICRR	0.439
HIST2H2BF	0.439
ATP6V0C	0.439
ARCN1	0.437
STIL	0.436
CACTIN	0.436
AKIRIN2	0.436
CDC6	0.436
RFXANK	0.436
TBC1D3	0.435
POLR2D	0.435
COPG1	0.433
EXOSC9	0.432
CCDC94	0.432
RPS5	0.432
PSMC6	0.431
RANBP2	0.431
XAB2	0.43
ORC6	0.429
CHD4	0.429
NPIPB8	0.428
POLA1	0.428
TTC31	0.428
GOLGA6A	0.427
PSMD11	0.427
NPIPB5	0.427
RPS3A	0.426
SLC38A7	0.426
DISP2	0.426
OSBP	0.426
SART1	0.423
RBBP5	0.423
EIF4A3	0.422
COPZ1	0.422
PLK4	0.422
MYB	0.421
TIMELESS	0.421
FAM160A1	0.421
CMYA5	0.42
NFATC4	0.42
CEP192	0.42
RPL9	0.42
TTC1	0.42
RPS28	0.419
NUP43	0.419
NUP153	0.418
C2orf27B	0.417
WDR87	0.417
GOLGA8O	0.417
GOLGA6L3	0.417
TBC1D3I	0.416
ATP6V0B	0.416
TRAPPC8	0.416
TBC1D3E	0.416
HGC6.3	0.416
PLA2G4F	0.416
ATP6V1A	0.416
MAT2A	0.415
TBC1D3K	0.415
RIOK2	0.415
FAM231A	0.415
TBC1D3L	0.415
FAM231C	0.415
AQR	0.415
SEL1L	0.412
LAD1	0.411
SMG1	0.411
RANGAP1	0.411
DHX38	0.41
RPS14	0.409
TOP1MT	0.409
RILPL2	0.409
SLC6A17	0.409
NUP107	0.408
SNIP1	0.408
HIST1H2BE	0.408
ATP13A1	0.407
GID4	0.406
CHAF1A	0.406
UBL5	0.406
SNRPA1	0.406
UTP3	0.406
RPL30	0.406
MMS22L	0.405
NOL12	0.405
BRF2	0.405
PLK1	0.405
DDT	0.405
RNPC3	0.405
POP1	0.404
VPS54	0.404
TONSL	0.404
ORC1	0.404
ANKLE1	0.404
DTL	0.403
NARFL	0.403
RPL34	0.403
PCF11	0.402
ANKLE2	0.402
PSMC1	0.402
ACBD4	0.401
AHCTF1	0.401
CGB2	0.401
PSMD7	0.401
GOLGA6B	0.401
PSMC4	0.4
IGFN1	0.4
NSF	0.4

Global Fraction of Cell Lines Where Essential: 738/739

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	28/28
blood	28/28
bone	26/26
breast	33/33
central nervous system	56/56
cervix	4/4
colorectal	17/17
esophagus	13/13
fibroblast	1/1
gastric	16/16
kidney	21/21
liver	20/20
lung	75/75
lymphocyte	16/16
ovary	26/26
pancreas	24/24
peripheral nervous system	16/16
plasma cell	15/15
prostate	1/1
skin	24/24
soft tissue	8/9
thyroid	2/2
upper aerodigestive	22/22
urinary tract	29/29
uterus	5/5

Essentiality in NALM6

Essentiality Rank: 110
Expression level (log2 read counts): 6.62

Expression Distribution

mitotic DNA replication preinitiation complex assembly
DNA replication preinitiation complex
regulation of chromatin silencing at telomere
DNA replication preinitiation complex assembly
nuclear pre-replicative complex
replication fork protection complex
mitotic DNA replication
double-strand break repair via break-induced replication
DNA replication checkpoint
3-5 DNA helicase activity
DNA replication origin binding
regulation of transcription involved in G1/S transition of mitotic cell cycle
DNA replication initiation
positive regulation of G1/S transition of mitotic cell cycle
regulation of chromatin silencing
nuclear DNA replication
cell cycle DNA replication
positive regulation of cell cycle G1/S phase transition
positive regulation of mitotic cell cycle phase transition
positive regulation of cell cycle phase transition
double-strand break repair via homologous recombination
recombinational repair
single-stranded DNA binding
DNA duplex unwinding
G1/S transition of mitotic cell cycle
DNA geometric change
cell cycle G1/S phase transition
DNA-dependent DNA replication
regulation of gene silencing
DNA integrity checkpoint
regulation of G1/S transition of mitotic cell cycle
positive regulation of mitotic cell cycle
regulation of cell cycle G1/S phase transition
double-strand break repair
regulation of chromatin organization
cell cycle checkpoint
protein-DNA complex assembly
DNA replication
DNA recombination
protein-DNA complex subunit organization