SUPT5H

Gene Information

Official Symbol: SUPT5H
Official Name: SPT5 homolog, DSIF elongation factor subunit
Aliases and Previous Symbols: N/A
Entrez ID: 6829
UniProt: O00267
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV- 1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. {ECO:0000269|PubMed:10075709, ECO:0000269|PubMed:10199401, ECO:0000269|PubMed:10393184, ECO:0000269|PubMed:10421630, ECO:0000269|PubMed:10454543, ECO:0000269|PubMed:10757782, ECO:0000269|PubMed:10912001, ECO:0000269|PubMed:11112772, ECO:0000269|PubMed:11553615, ECO:0000269|PubMed:11809800, ECO:0000269|PubMed:12653964, ECO:0000269|PubMed:12718890, ECO:0000269|PubMed:14701750, ECO:0000269|PubMed:15136722, ECO:0000269|PubMed:15380072, ECO:0000269|PubMed:16214896, ECO:0000269|PubMed:9450929, ECO:0000269|PubMed:9514752, ECO:0000269|PubMed:9857195}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
Pimelic-diphenylamide-106 5μM R00 exp28	-1.89
Etoposide 0.3μM R05 exp199	-1.72
Pimelic-diphenylamide-106 0.5μM R00 exp27	1.71
HMS-I2 5μM R06 exp287	2.18
Pifithrin-alpha 20μM R07 exp390	2.21
ABT-702 5μM plus CoCl2 18μM R07 exp320	2.27

Gene	Correlation
RPL35	0.574
PSMB4	0.571
CHD4	0.569
CBWD3	0.56
TCEB3C	0.559
TCEB3CL	0.546
TBC1D3G	0.539
TBC1D3H	0.538
TBC1D3F	0.537
TICRR	0.536
C14orf93	0.536
NPIPB5	0.535
SNRPA1	0.534
EIF4A3	0.533
TCEB3CL2	0.532
SUPT6H	0.532
CBWD5	0.532
TFIP11	0.53
CBWD6	0.529
NUP160	0.524
C1QTNF9B	0.521
STIL	0.52
COPG1	0.518
VASN	0.516
POLR2J3	0.516
SLC4A1	0.516
KRT78	0.514
COPB1	0.514
IK	0.513
ATP6V1C1	0.513
RRN3	0.509
NAPA	0.508
AQR	0.508
CEP192	0.507
TBC1D3	0.507
TBC1D3C	0.506
CDC6	0.506
POLE	0.505
OSBP	0.504
DTL	0.503
CACTIN	0.502
ISCU	0.5
AATF	0.498
PDE4DIP	0.498
EXOSC9	0.498
ARCN1	0.497
PSMD3	0.495
SF3A1	0.493
DOCK6	0.492
RAD51	0.491
ATP6AP1	0.491
RRP7A	0.49
ZNF655	0.489
PAN3	0.489
POLR2A	0.488
POLR2D	0.487
TUBA8	0.487
CGB2	0.487
TBC1D3E	0.486
TBC1D3K	0.486
TBC1D3L	0.486
TBC1D3I	0.486
PSMB2	0.484
PSMA6	0.483
VPS28	0.483
CCNA2	0.482
MBTD1	0.481
NUPL1	0.48
ACSF2	0.477
TP53TG3B	0.477
AAMP	0.477
RPS19BP1	0.476
TP53TG3	0.476
GOLGA8O	0.476
ANKLE2	0.475
TP53TG3C	0.475
CDC45	0.475
MT1E	0.475
TSG101	0.474
SART1	0.473
COPZ1	0.473
GID4	0.47
TSPY8	0.469
ATP6V0C	0.469
GAR1	0.468
IGFN1	0.467
TIMELESS	0.467
RPS2	0.466
PRIM1	0.466
LRR1	0.464
EXOSC5	0.464
UTP20	0.464
XAB2	0.464
SLC6A17	0.464
POLA1	0.461
RPS5	0.46
CHAF1A	0.46
MYT1L	0.46
INTS5	0.459
TONSL	0.459
SNIP1	0.459
ATP6V1B2	0.457
SEC13	0.457
NUP85	0.456
PLK1	0.456
SNRNP70	0.455
TP53TG3D	0.455
PRPF19	0.455
OR10G3	0.454
FAM160A1	0.454
PSMA7	0.453
SF3A2	0.453
RAD21	0.453
RBM39	0.452
DISP2	0.452
CHMP4B	0.452
CLN6	0.452
VPS54	0.452
ATP6V0B	0.451
PSMC1	0.45
OGFR	0.45
GOLGA6L6	0.449
COPA	0.449
FAM20A	0.449
UTP3	0.448
RRM2	0.447
GOLGA8J	0.446
CDCA3	0.446
TWISTNB	0.445
PWP2	0.445
MYB	0.444
ATP6V1A	0.443
CDC73	0.443
RPS12	0.441
WDR75	0.441
PAF1	0.44
BYSL	0.44
PBXIP1	0.44
TPX2	0.439
PSMB3	0.439
GPRIN2	0.439
XYLT2	0.438
NARFL	0.438
DDTL	0.438
HINFP	0.438
ORC6	0.437
RBBP5	0.437
GOLGA6L3	0.436
SF3B2	0.436
SRRT	0.435
COPE	0.435
NUP205	0.434
PSMB1	0.434
POLR1A	0.434
TYMS	0.433
BRD2	0.433
DDX23	0.433
COPB2	0.432
NUP153	0.432
GOLGA8H	0.431
POLR2F	0.431
FAM231C	0.43
FAM231A	0.43
CRNKL1	0.43
POLR2J	0.43
HAP1	0.429
GOLGA6L4	0.429
CCDC64B	0.429
RPL10A	0.429
RPL34	0.428
CEBPZ	0.427
NOL10	0.427
EGR2	0.427
MED17	0.427
RNPC3	0.426
NOP56	0.426
GOLGA8K	0.425
RPS20	0.424
MDM2	0.424
PPIE	0.424
SF3A3	0.424
KIF3C	0.423
ORC1	0.423
EXOSC6	0.423
LAD1	0.422
RPSA	0.422
EXOSC3	0.422
MSH5	0.421
PCF11	0.421
PSMC5	0.42
DDX56	0.42
MED15	0.42
PLK4	0.419
ZNF106	0.419
ARMC8	0.419
ZFAND5	0.419
RANBP2	0.418
RRP15	0.418
ZNF574	0.418
SAP30BP	0.418
CDH24	0.417
HIST2H2BF	0.417
TRAPPC11	0.417
ANKLE1	0.417
RPL8	0.417
UPF1	0.416
NFYA	0.416
UBAP1	0.416
NUP54	0.416
ALDH1B1	0.416
PEX5	0.415
NSF	0.414
RPP40	0.413
RPS9	0.413
CWC22	0.413
PSMD6	0.412
RPL12	0.412
NDC80	0.412
HIVEP3	0.412
THOC1	0.412
PSMC2	0.411
HIST1H2BE	0.411
MYO18A	0.411
POLR2H	0.411
RPS19	0.411
DNTTIP2	0.41
SULT2B1	0.41
NIPBL	0.41
MADCAM1	0.409
COX7A2L	0.409
RPS3A	0.409
KCTD17	0.409
RPS7	0.409
NOL12	0.409
MFSD12	0.408
NPIPB8	0.408
RILPL2	0.408
ZNF830	0.408
SEMA6B	0.408
MMS22L	0.407
RPL6	0.407
C2orf27B	0.406
POLD1	0.406
KRT2	0.406
SKIV2L2	0.405
SSH2	0.405
MYH2	0.404
UQCRH	0.404
CHMP2A	0.404
TGFBRAP1	0.404
PPFIA4	0.403
HIST2H3C	0.403
HIST2H3A	0.403
DIDO1	0.403
EXD1	0.403
TAF15	0.403
MPHOSPH10	0.403
TINF2	0.402
PSMD7	0.402
TAGLN	0.401
PAN2	0.4
PSMD8	0.4

Global Fraction of Cell Lines Where Essential: 698/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	1/1
909776.0	1/1
bile duct	24/28
blood	27/28
bone	23/25
breast	31/33
central nervous system	54/56
cervix	4/4
colorectal	16/17
esophagus	13/13
fibroblast	1/1
gastric	15/15
kidney	21/21
liver	20/20
lung	72/75
lymphocyte	14/14
ovary	25/26
pancreas	22/24
peripheral nervous system	16/16
plasma cell	14/15
prostate	1/1
skin	24/24
soft tissue	7/7
thyroid	2/2
upper aerodigestive	22/22
urinary tract	27/29
uterus	4/5

Essentiality in NALM6

Essentiality Rank: 987
Expression level (log2 read counts): 7.36

Expression Distribution

Spt5-NGN
Spt5 N
KOW
CTD

DSIF complex
negative regulation of mRNA polyadenylation
negative regulation of mRNA 3-end processing
negative regulation of DNA-templated transcription, elongation
regulation of mRNA polyadenylation
regulation of mRNA 3-end processing
negative regulation of mRNA processing
positive regulation of DNA-templated transcription, elongation
7-methylguanosine mRNA capping
7-methylguanosine RNA capping
RNA capping
positive regulation of viral transcription
regulation of DNA-templated transcription, elongation
regulation of viral transcription
positive regulation of macroautophagy
transcription elongation from RNA polymerase II promoter
negative regulation of mRNA metabolic process
DNA-templated transcription, elongation
positive regulation of viral process
positive regulation of autophagy
regulation of mRNA processing
mRNA binding
regulation of macroautophagy
regulation of viral process
regulation of symbiosis, encompassing mutualism through parasitism
regulation of mRNA metabolic process
regulation of autophagy
enzyme binding
positive regulation of cellular catabolic process
chromatin binding
positive regulation of catabolic process
mRNA processing
transcription by RNA polymerase II
protein heterodimerization activity
positive regulation of multi-organism process
transcription, DNA-templated
nucleic acid-templated transcription
RNA biosynthetic process
mRNA metabolic process
regulation of multi-organism process