BRD4

Gene Information

Official Symbol: BRD4
Official Name: bromodomain containing 4
Aliases and Previous Symbols: N/A
Entrez ID: 23476
UniProt: O60885
Interactions: BioGRID
PubMed articles: Open PubMed
OMIM: Open OMIM

Function Summary

Entrez Summary: N/A
UniProt Summary: Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P- TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P- TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters. {ECO:0000269|PubMed:22509028}.

Pfam Domains GO Terms

CRISPR Data

Compound Hit Most Correlated Genes in Chemogenomics Tissues where Essential in the Avana Dataset (DepMap 20Q1)

Screen	Score
INK128 0.2μM R02 exp70	-1.81
Cycloheximide 0.2μM R00 exp15	1.72
Dihydrosphingosine 8μM R08 exp476	1.72
Methotrexate 0.01μM R08 exp501	1.76
Mdivi-1 15μM R05 exp217	1.79
CB-839 10μM R05 exp224	1.81
Cycloheximide 0.02μM R00 exp14	1.82
GSK591 2.6μM R03 exp128	1.86
Hypoxia 5%O2 R04 exp191	2
ABT-702 5μM R07 exp318	2.04
Pyridostatin 4μM R05 exp240	2.11
Parthenolide 2.5μM R05 exp238	2.23

Global Fraction of Cell Lines Where Essential: 519/726

Tissue	Fraction Of Cell Lines Where Essential
1290807.0	0/1
909776.0	1/1
bile duct	24/28
blood	21/28
bone	18/25
breast	20/33
central nervous system	38/56
cervix	2/4
colorectal	12/17
esophagus	10/13
fibroblast	1/1
gastric	11/15
kidney	11/21
liver	15/20
lung	53/75
lymphocyte	12/14
ovary	19/26
pancreas	17/24
peripheral nervous system	10/16
plasma cell	14/15
prostate	1/1
skin	20/24
soft tissue	3/7
thyroid	2/2
upper aerodigestive	14/22
urinary tract	21/29
uterus	4/5

Essentiality in NALM6

Essentiality Rank: 744
Expression level (log2 read counts): 7.07

Expression Distribution

Gene	Correlation
TBC1D3H	0.452
TBC1D3G	0.43
PRPF8	0.423
SFPQ	0.415
POLR2J3	0.408
KIF3C	0.405
PRIM1	0.402

positive regulation of histone H3-K36 trimethylation
P-TEFb complex binding
regulation of phosphorylation of RNA polymerase II C-terminal domain
transcription regulator recruiting activity
positive regulation of histone H3-K36 methylation
regulation of histone H3-K36 trimethylation
RNA polymerase II C-terminal domain binding
regulation of histone H3-K36 methylation
RNA polymerase II CTD heptapeptide repeat kinase activity
positive regulation of transcription elongation from RNA polymerase II promoter
lysine-acetylated histone binding
positive regulation of G2/M transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
condensed nuclear chromosome
positive regulation of cell cycle G2/M phase transition
regulation of transcription elongation from RNA polymerase II promoter
positive regulation of DNA-templated transcription, elongation
positive regulation of histone methylation
regulation of DNA-templated transcription, elongation
regulation of histone methylation
p53 binding
positive regulation of mitotic cell cycle phase transition
positive regulation of histone modification
positive regulation of cell cycle phase transition
positive regulation of chromatin organization
G1/S transition of mitotic cell cycle
cell cycle G1/S phase transition
regulation of histone modification
positive regulation of mitotic cell cycle
positive regulation of chromosome organization
positive regulation of I-kappaB kinase/NF-kappaB signaling
regulation of chromatin organization
regulation of G2/M transition of mitotic cell cycle
regulation of cell cycle G2/M phase transition
regulation of I-kappaB kinase/NF-kappaB signaling
mitotic cell cycle phase transition
cell cycle phase transition
positive regulation of cell cycle process
regulation of inflammatory response
enzyme binding